Development of a RT-qPCR method for the quantification of Fibrobacter succinogenes S85 glycoside hydrolase transcripts in the rumen content of gnotobiotic and conventional sheep

Journal of Microbiological Methods

Volumn 77, Issue 1, 2009, Pages 8-16

  Béra Maillet, Christel ^a   Mosoni, Pascale ^a   Kwasiborski, Anthony ^a   Suau, Florent ^a   Ribot, Yves ^a   Forano, Evelyne ^a  

^a CEMAGREF   (France)

Author keywords

Fibrobacter succinogenes; Glycoside hydrolase; Gnotobiotic lamb; RNA isolation; RT qPCR; Rumen

Indexed keywords

CELLODEXTRINASE CEDA; DEXTRIN; ELONGATION FACTOR; ELONGATION FACTOR TUF; ENDOGLUCANASE CEL3; ENDOGLUCANASE CELF; ENDOGLUCANASE ENDA; ENDOGLUCANASE ENDB; GLUCAN GLUCOSIDASE; GLUCAN SYNTHASE; GLYCOSIDASE; LICHENASE MLG; RIBOSOME RNA; RNA; UNCLASSIFIED DRUG; XYLAN ENDO 1,3 BETA XYLOSIDASE; XYLANASE XYNC; XYLANASE XYND;

ANIMAL EXPERIMENT; ARTICLE; BACTERIAL STRAIN; CONTROLLED STUDY; CONVENTIONAL SHEEP; FIBROBACTER SUCCINOGENES; GENE EXPRESSION; GENE MUTATION; GENETIC TRANSCRIPTION; GNOTOBIOTICS; LAMB; MICROFLORA; MICROORGANISM; NONHUMAN; PRIORITY JOURNAL; QUANTITATIVE ASSAY; REVERSE TRANSCRIPTION POLYMERASE CHAIN REACTION; RNA EXTRACTION; RNA ISOLATION; RUMINANT STOMACH; SHEEP;

ANIMALS; BACTERIAL PROTEINS; FIBROBACTER; GERM-FREE LIFE; GLYCOSIDE HYDROLASES; REVERSE TRANSCRIPTASE POLYMERASE CHAIN REACTION; RUMEN; SHEEP; TRANSCRIPTION, GENETIC;

ANIMALIA; BACTERIA (MICROORGANISMS); FIBROBACTER SUCCINOGENES; OVIS ARIES;

EID: 62349131091     PISSN: 01677012     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.mimet.2008.11.009     Document Type: Article

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