메뉴 건너뛰기




Volumn 103, Issue 6, 2007, Pages 2676-2685

Quantification by real-time PCR of cellulolytic bacteria in the rumen of sheep after supplementation of a forage diet with readily fermentable carbohydrates: Effect of a yeast additive

Author keywords

Cellulolytic bacteria; Diet; Real time PCR; Rumen; Yeast

Indexed keywords

ADDITIVES; BACTERIA; CARBOHYDRATES; POLYMERASE CHAIN REACTION;

EID: 36549009595     PISSN: 13645072     EISSN: 13652672     Source Type: Journal    
DOI: 10.1111/j.1365-2672.2007.03517.x     Document Type: Article
Times cited : (145)

References (48)
  • 2
    • 1842509949 scopus 로고    scopus 로고
    • Strain-specific genomic regions of Ruminococcus flavefaciens FD-1 as revealed by combinatorial random-phase genome sequencing and suppressive subtractive hybridization
    • Antonopoulos, D.A., Nelson, K.E., Morrison, M. White, B.A. (2004) Strain-specific genomic regions of Ruminococcus flavefaciens FD-1 as revealed by combinatorial random-phase genome sequencing and suppressive subtractive hybridization. Environ Microbiol 6, 335 346.
    • (2004) Environ Microbiol , vol.6 , pp. 335-346
    • Antonopoulos, D.A.1    Nelson, K.E.2    Morrison, M.3    White, B.A.4
  • 3
    • 34249024952 scopus 로고    scopus 로고
    • Daily rumen pH pattern of loose-housed dairy cattle as affected by feeding pattern and live yeast supplementation
    • Bach, A., Iglesias, C. Devant, M. (2006) Daily rumen pH pattern of loose-housed dairy cattle as affected by feeding pattern and live yeast supplementation. Anim Feed Sci Technol 136, 146 153.
    • (2006) Anim Feed Sci Technol , vol.136 , pp. 146-153
    • Bach, A.1    Iglesias, C.2    Devant, M.3
  • 4
    • 13844312429 scopus 로고    scopus 로고
    • Fiber-degrading systems of different strains of the genus Fibrobacter
    • Béra-Maillet, C., Ribot, Y. Forano, E. (2004) Fiber-degrading systems of different strains of the genus Fibrobacter. Appl Environ Microbiol 70, 2172 2179.
    • (2004) Appl Environ Microbiol , vol.70 , pp. 2172-2179
    • Béra-Maillet, C.1    Ribot, Y.2    Forano, E.3
  • 6
    • 85010245816 scopus 로고
    • Cultural methods and some characteristics of some of the more numerous groups of bacteria in the bovine rumen
    • Bryant, M.P. Burkey, L.A. (1953) Cultural methods and some characteristics of some of the more numerous groups of bacteria in the bovine rumen. J Dairy Science 36, 205 217.
    • (1953) J Dairy Science , vol.36 , pp. 205-217
    • Bryant, M.P.1    Burkey, L.A.2
  • 7
    • 0035026397 scopus 로고    scopus 로고
    • Establishment of cellulolytic bacteria and development of fermentative activities in the rumen of gnotobiotically-reared lambs receiving the microbial additive Saccharomyces cerevisiae Cncm I-1077
    • Chaucheyras-Durand, F. Fonty, G. (2001) Establishment of cellulolytic bacteria and development of fermentative activities in the rumen of gnotobiotically-reared lambs receiving the microbial additive Saccharomyces cerevisiae Cncm I-1077. Reprod Nutr Dev 41, 57 68.
    • (2001) Reprod Nutr Dev , vol.41 , pp. 57-68
    • Chaucheyras-Durand, F.1    Fonty, G.2
  • 8
    • 0036231875 scopus 로고    scopus 로고
    • Influence of a probiotic yeast (Saccharomyces cerevisiae CNCM I-1077) on microbial colonization and fermentation in the rumen of newborn lambs
    • Chaucheyras-Durand, F. Fonty, G. (2002) Influence of a probiotic yeast (Saccharomyces cerevisiae CNCM I-1077) on microbial colonization and fermentation in the rumen of newborn lambs. Microb Ecol Health Dis 14, 30 36.
    • (2002) Microb Ecol Health Dis , vol.14 , pp. 30-36
    • Chaucheyras-Durand, F.1    Fonty, G.2
  • 9
    • 33947179657 scopus 로고    scopus 로고
    • Effect of the addition of Levucell SC on the rumen microflora of sheep during adaptation to high starch diets
    • Chaucheyras, F., Millet, L., Michalet-Doreau, B., Fonty, G., Bertin, G. Gouet, P. (1997) Effect of the addition of Levucell SC on the rumen microflora of sheep during adaptation to high starch diets. Reprod Nutr Dev EE5, 82 83.
    • (1997) Reprod Nutr Dev , vol.5 , pp. 82-83
    • Chaucheyras, F.1    Millet, L.2    Michalet-Doreau, B.3    Fonty, G.4    Bertin, G.5    Gouet, P.6
  • 10
    • 0020967449 scopus 로고
    • A simple and versatile micro-computer program for the determination of "most Probable Number"
    • Clarke, K.R. Owens, N.J.P. (1983) A simple and versatile micro-computer program for the determination of "Most Probable Number". J Microbiol Methods 1, 133 137.
    • (1983) J Microbiol Methods , vol.1 , pp. 133-137
    • Clarke, K.R.1    Owens, N.J.P.2
  • 11
    • 0024440472 scopus 로고
    • Most-probable-number procedures for enumerating ruminal bacteria, including the simultaneous estimation of total and cellulolytic numbers in one medium
    • Dehority, B.A., Tirabasso, P.A. Grifo, A.P. Jr. (1989) Most-probable-number procedures for enumerating ruminal bacteria, including the simultaneous estimation of total and cellulolytic numbers in one medium. Appl Environ Microbiol 55, 2789 2792.
    • (1989) Appl Environ Microbiol , vol.55 , pp. 2789-2792
    • Dehority, B.A.1    Tirabasso, P.A.2    Grifo Jr., A.P.3
  • 12
    • 33751016342 scopus 로고    scopus 로고
    • Development of a real-time PCR assay for monitoring anaerobic fungal and cellulolytic bacterial populations within the rumen
    • Denman, S.E. McSweeney, C.S. (2006) Development of a real-time PCR assay for monitoring anaerobic fungal and cellulolytic bacterial populations within the rumen. FEMS Microbiol Ecol 58, 572 582.
    • (2006) FEMS Microbiol Ecol , vol.58 , pp. 572-582
    • Denman, S.E.1    McSweeney, C.S.2
  • 13
    • 0011915231 scopus 로고
    • Effect of a yeast culture on the growth characteristics of representative ruminal bacteria
    • Girard, I.D. Dawson, K.A. (1995) Effect of a yeast culture on the growth characteristics of representative ruminal bacteria. J Anim Sci 73, 264.
    • (1995) J Anim Sci , vol.73 , pp. 264
    • Girard, I.D.1    Dawson, K.A.2
  • 14
    • 0000879885 scopus 로고
    • The cellulolytic activity of pure strains of bacteria from the rumen of cattle
    • Halliwell, G. Bryant, M.P. (1963) The cellulolytic activity of pure strains of bacteria from the rumen of cattle. J Gen Microbiol 32, 441 448.
    • (1963) J Gen Microbiol , vol.32 , pp. 441-448
    • Halliwell, G.1    Bryant, M.P.2
  • 15
    • 0020827852 scopus 로고
    • Effect of soluble carbohydrates on digestion of cellulose by pure cultures of rumen bacteria
    • Hiltner, P. Dehority, B.A. (1983) Effect of soluble carbohydrates on digestion of cellulose by pure cultures of rumen bacteria. Appl Environ Microbiol 46, 642 648.
    • (1983) Appl Environ Microbiol , vol.46 , pp. 642-648
    • Hiltner, P.1    Dehority, B.A.2
  • 16
    • 0008346972 scopus 로고
    • Methods of manipulating the microbial metabolism in the rumen
    • Jouany, J.P. (1994) Methods of manipulating the microbial metabolism in the rumen. Ann Zootech 43, 49 62.
    • (1994) Ann Zootech , vol.43 , pp. 49-62
    • Jouany, J.P.1
  • 17
    • 0347403721 scopus 로고    scopus 로고
    • Manipulation of the rumen ecosystem to support high-performance beef cattle - Review
    • Jouany, J.P., Michalet-Doreau, B. Doreau, M. (2000) Manipulation of the rumen ecosystem to support high-performance beef cattle - review. Asian-Aus J Anim Sci 13, 96 114.
    • (2000) Asian-Aus J Anim Sci , vol.13 , pp. 96-114
    • Jouany, J.P.1    Michalet-Doreau, B.2    Doreau, M.3
  • 18
    • 0042882236 scopus 로고    scopus 로고
    • Establishing populations of Megasphaera elsdenii YE 34 and Butyrivibrio fibrisolvens YE 44 in the rumen of cattle fed high grain diets
    • Klieve, A.V., Hennessy, D., Ouwerkerk, D., Forster, R.J., Mackie, R.I. Attwood, G.T. (2003) Establishing populations of Megasphaera elsdenii YE 34 and Butyrivibrio fibrisolvens YE 44 in the rumen of cattle fed high grain diets. J Appl Microbiol 95, 621 630.
    • (2003) J Appl Microbiol , vol.95 , pp. 621-630
    • Klieve, A.V.1    Hennessy, D.2    Ouwerkerk, D.3    Forster, R.J.4    MacKie, R.I.5    Attwood, G.T.6
  • 19
    • 4344684794 scopus 로고    scopus 로고
    • Recent advances in gut microbiology and their possible contribution to animal health and production - A review
    • Kobayashi, Y., Koike, S., Taguchi, H., Itabashi, H., Kam, D.K. Ha, J.K. (2004) Recent advances in gut microbiology and their possible contribution to animal health and production - a review. Asian-Aus J Anim Sci 17, 877 884.
    • (2004) Asian-Aus J Anim Sci , vol.17 , pp. 877-884
    • Kobayashi, Y.1    Koike, S.2    Taguchi, H.3    Itabashi, H.4    Kam, D.K.5    Ha, J.K.6
  • 20
    • 0035856590 scopus 로고    scopus 로고
    • Development and use of competitive PCR assays for the rumen cellulolytic bacteria: Fibrobacter succinogenes, Ruminococcus albus and Ruminococcus flavefaciens
    • Koike, S. Kobayashi, Y. (2001) Development and use of competitive PCR assays for the rumen cellulolytic bacteria: Fibrobacter succinogenes, Ruminococcus albus and Ruminococcus flavefaciens. FEMS Microbiol Lett 204, 361 366.
    • (2001) FEMS Microbiol Lett , vol.204 , pp. 361-366
    • Koike, S.1    Kobayashi, Y.2
  • 21
    • 0038749347 scopus 로고    scopus 로고
    • Kinetics of in sacco fiber-attachment of representative ruminal cellulolytic bacteria monitored by competitive PCR
    • Koike, S., Pan, J., Kobayashi, Y. Tanaka, K. (2003) Kinetics of in sacco fiber-attachment of representative ruminal cellulolytic bacteria monitored by competitive PCR. J Dairy Sci 86, 1429 1435.
    • (2003) J Dairy Sci , vol.86 , pp. 1429-1435
    • Koike, S.1    Pan, J.2    Kobayashi, Y.3    Tanaka, K.4
  • 22
    • 9644289655 scopus 로고    scopus 로고
    • Ruminal distribution of the cellulolytic bacterium Fibrobacter succinogenes in relation to its phylogenetic grouping
    • Koike, S., Pan, J., Suzuki, T., Takano, T., Oshima, C., Kobayashi, Y. Tanaka, K. (2004) Ruminal distribution of the cellulolytic bacterium Fibrobacter succinogenes in relation to its phylogenetic grouping. Anim Sci J 75, 417 422.
    • (2004) Anim Sci J , vol.75 , pp. 417-422
    • Koike, S.1    Pan, J.2    Suzuki, T.3    Takano, T.4    Oshima, C.5    Kobayashi, Y.6    Tanaka, K.7
  • 23
    • 0032805304 scopus 로고    scopus 로고
    • 16S rDNA sequencing of Ruminococcus albus and Ruminococcus flavefaciens: Design of a signature probe and its application in adult sheep
    • Krause, D.O., Dalrymple, B.P., Smith, W.J., Mackie, R.I. McSweeney, C.S. (1999) 16S rDNA sequencing of Ruminococcus albus and Ruminococcus flavefaciens: design of a signature probe and its application in adult sheep. Microbiology 145, 1797 1807.
    • (1999) Microbiology , vol.145 , pp. 1797-1807
    • Krause, D.O.1    Dalrymple, B.P.2    Smith, W.J.3    MacKie, R.I.4    McSweeney, C.S.5
  • 25
    • 4844229645 scopus 로고    scopus 로고
    • Use of community genome arrays (CGAs) to assess the effects of Acacia angustissima on rumen ecology
    • Krause, D.O., Smith, W.J. McSweeney, C.S. (2004) Use of community genome arrays (CGAs) to assess the effects of Acacia angustissima on rumen ecology. Microbiology 150, 2899 2909.
    • (2004) Microbiology , vol.150 , pp. 2899-2909
    • Krause, D.O.1    Smith, W.J.2    McSweeney, C.S.3
  • 26
    • 0018957077 scopus 로고
    • Differential carbohydrate media and anaerobic replica plating techniques in delineating carbohydrate-utilizing subgroups in rumen bacterial populations
    • Leedle, J.A. Hespell, R.B. (1980) Differential carbohydrate media and anaerobic replica plating techniques in delineating carbohydrate-utilizing subgroups in rumen bacterial populations. Appl Environ Microbiol 39, 709 719.
    • (1980) Appl Environ Microbiol , vol.39 , pp. 709-719
    • Leedle, J.A.1    Hespell, R.B.2
  • 27
    • 0042084317 scopus 로고    scopus 로고
    • Factors affecting the fibrolytic activity of the digestive microbial ecosystems in ruminants
    • In. ed. Martin, S.A. pp. India: Research Signpost.
    • Martin, C., Fonty, G. Michalet-Doreau, B. (2002) Factors affecting the fibrolytic activity of the digestive microbial ecosystems in ruminants. In Gastrointestinal Microbiology in Animals ed. Martin, S.A. pp. 1 17. India : Research Signpost.
    • (2002) Gastrointestinal Microbiology in Animals , pp. 1-17
    • Martin, C.1    Fonty, G.2    Michalet-Doreau, B.3
  • 28
    • 0035736528 scopus 로고    scopus 로고
    • Cereal supplementation modified the fibrolytic activity but not the structure of the cellulolytic bacterial community associated with rumen solid digesta
    • Martin, C., Millet, L., Fonty, G. Michalet-Doreau, B. (2001) Cereal supplementation modified the fibrolytic activity but not the structure of the cellulolytic bacterial community associated with rumen solid digesta. Reprod Nutr Dev 41, 413 424.
    • (2001) Reprod Nutr Dev , vol.41 , pp. 413-424
    • Martin, C.1    Millet, L.2    Fonty, G.3    Michalet-Doreau, B.4
  • 29
    • 0036518881 scopus 로고    scopus 로고
    • A comparison of enzymatic and molecular approaches to characterize the cellulolytic microbial ecosystems of the rumen and the cecum
    • Michalet-Doreau, B., Fernandez, I. Fonty, G. (2002) A comparison of enzymatic and molecular approaches to characterize the cellulolytic microbial ecosystems of the rumen and the cecum. J Anim Sci 80, 790 796.
    • (2002) J Anim Sci , vol.80 , pp. 790-796
    • Michalet-Doreau, B.1    Fernandez, I.2    Fonty, G.3
  • 30
    • 67349100788 scopus 로고    scopus 로고
    • Effect of yeast culture, Saccharomyces cerevisiae SC CNCM I-1077, on ruminal fermentation during adaptation to high-concentrate feeding
    • In. pp. 121. Paris, France.
    • Michalet-Doreau, B. Morand, D. (1997) Effect of yeast culture, Saccharomyces cerevisiae SC CNCM I-1077, on ruminal fermentation during adaptation to high-concentrate feeding. In Proceedings from the 4th «Rencontres autour des Recherches sur les Ruminants» pp. 121. Paris, France.
    • (1997) Proceedings from the 4th «rencontres Autour des Recherches Sur Les Ruminants»
    • Michalet-Doreau, B.1    Morand, D.2
  • 31
    • 36549006492 scopus 로고    scopus 로고
    • Effect of the microbial additive Levucell SC on microbial activity in the rumen during the stepwise adaptation of sheep to high concentrate diet
    • Michalet-Doreau, B., Morand, D. Martin, C. (1997) Effect of the microbial additive Levucell SC on microbial activity in the rumen during the stepwise adaptation of sheep to high concentrate diet. Reprod Nutr Dev E EE5, 82 83.
    • (1997) Reprod Nutr Dev e , vol.5 , pp. 82-83
    • Michalet-Doreau, B.1    Morand, D.2    Martin, C.3
  • 32
    • 0026979966 scopus 로고
    • Effects of extracellular pH on the intracellular pH and membrane potential of cellulolytic ruminal bacteria, Ruminococcus albus, Ruminococcus flavefaciens, and Fibrobacter succinogenes
    • Miyazaki, K., Hino, T. Itabashi, H. (1992) Effects of extracellular pH on the intracellular pH and membrane potential of cellulolytic ruminal bacteria, Ruminococcus albus, Ruminococcus flavefaciens, and Fibrobacter succinogenes. J gen appl microbiol 38, 567 573.
    • (1992) J Gen Appl Microbiol , vol.38 , pp. 567-573
    • Miyazaki, K.1    Hino, T.2    Itabashi, H.3
  • 33
    • 0036240224 scopus 로고    scopus 로고
    • Enumeration of Megasphaera elsdenii in rumen contents by real-time Taq nuclease assay
    • Ouwerkerk, D., Klieve, A.V. Forster, R.J. (2002) Enumeration of Megasphaera elsdenii in rumen contents by real-time Taq nuclease assay. J Appl Microbiol 92, 753 758.
    • (2002) J Appl Microbiol , vol.92 , pp. 753-758
    • Ouwerkerk, D.1    Klieve, A.V.2    Forster, R.J.3
  • 34
    • 32944463559 scopus 로고    scopus 로고
    • Real-time PCR detection of the effects of protozoa on rumen bacteria in cattle
    • Ozutsumi, Y., Tajima, K., Takenaka, A. Itabashi, H. (2006) Real-time PCR detection of the effects of protozoa on rumen bacteria in cattle. Curr Microbiol 52, 158 162.
    • (2006) Curr Microbiol , vol.52 , pp. 158-162
    • Ozutsumi, Y.1    Tajima, K.2    Takenaka, A.3    Itabashi, H.4
  • 35
    • 18944387901 scopus 로고    scopus 로고
    • Novel molecular features of the fibrolytic intestinal bacterium Fibrobacter intestinalis not shared with Fibrobacter succinogenes as determined by suppressive subtractive hybridization
    • Qi, M., Nelson, K.E., Daugherty, S.C., Nelson, W.C., Hance, I.R., Morrison, M. Forsberg, C.W. (2005) Novel molecular features of the fibrolytic intestinal bacterium Fibrobacter intestinalis not shared with Fibrobacter succinogenes as determined by suppressive subtractive hybridization. J Bacteriol 187, 3739 3751.
    • (2005) J Bacteriol , vol.187 , pp. 3739-3751
    • Qi, M.1    Nelson, K.E.2    Daugherty, S.C.3    Nelson, W.C.4    Hance, I.R.5    Morrison, M.6    Forsberg, C.W.7
  • 37
    • 0030203318 scopus 로고    scopus 로고
    • Why are ruminal cellulolytic bacteria unable to digest cellulose at low PH?
    • Russell, J.B. Wilson, D.B. (1996) Why are ruminal cellulolytic bacteria unable to digest cellulose at low PH? J Dairy Sci 79, 1503 1509.
    • (1996) J Dairy Sci , vol.79 , pp. 1503-1509
    • Russell, J.B.1    Wilson, D.B.2
  • 38
    • 33644870885 scopus 로고    scopus 로고
    • Development and validation of a real-time PCR method to quantify rumen protozoa and examination of variability between entodinium populations in sheep offered a hay-based diet
    • Skillman, L.C., Toovey, A.F., Williams, A.J. Wright, A.D. (2006) Development and validation of a real-time PCR method to quantify rumen protozoa and examination of variability between entodinium populations in sheep offered a hay-based diet. Appl Environ Microbiol 72, 200 206.
    • (2006) Appl Environ Microbiol , vol.72 , pp. 200-206
    • Skillman, L.C.1    Toovey, A.F.2    Williams, A.J.3    Wright, A.D.4
  • 39
    • 0024005650 scopus 로고
    • Use of phylogenetically based hybridization probes for studies of ruminal microbial ecology
    • Stahl, D.A., Flesher, B., Mansfield, H.R. Montgomery, L. (1988) Use of phylogenetically based hybridization probes for studies of ruminal microbial ecology. Appl Environ Microbiol 54, 1079 1084.
    • (1988) Appl Environ Microbiol , vol.54 , pp. 1079-1084
    • Stahl, D.A.1    Flesher, B.2    Mansfield, H.R.3    Montgomery, L.4
  • 40
    • 34247620908 scopus 로고    scopus 로고
    • Dominance of Prevotella and low abundance of classical ruminal bacterial species in the bovine rumen revealed by relative quantification real-time PCR
    • Stevenson, D.M. Weimer, P.J. (2007) Dominance of Prevotella and low abundance of classical ruminal bacterial species in the bovine rumen revealed by relative quantification real-time PCR. Appl Microbiol Biotechnol 75, 165 174.
    • (2007) Appl Microbiol Biotechnol , vol.75 , pp. 165-174
    • Stevenson, D.M.1    Weimer, P.J.2
  • 41
    • 0017345684 scopus 로고
    • Factors affecting the cellulolytic activity of rumen contents
    • Stewart, C.S. (1977) Factors affecting the cellulolytic activity of rumen contents. Appl Environ Microbiol 33, 497 502.
    • (1977) Appl Environ Microbiol , vol.33 , pp. 497-502
    • Stewart, C.S.1
  • 42
    • 0003185856 scopus 로고
    • The rumen bacteria
    • In. ed. Hobson, P.N. pp. London: Elsevier Applied Science.
    • Stewart, C.S. Bryant, M.P. (1988) The rumen bacteria. In The Rumen Microbial Ecosystem ed. Hobson, P.N. pp. 21 26. London : Elsevier Applied Science.
    • (1988) The Rumen Microbial Ecosystem , pp. 21-26
    • Stewart, C.S.1    Bryant, M.P.2
  • 43
    • 10044284420 scopus 로고    scopus 로고
    • Development of an assay to quantify rumen ciliate protozoal biomass in cows using real-time PCR
    • Sylvester, J.T., Karnati, S.K., Yu, Z., Morrison, M. Firkins, J.L. (2004) Development of an assay to quantify rumen ciliate protozoal biomass in cows using real-time PCR. J Nutr 134, 3378 3384.
    • (2004) J Nutr , vol.134 , pp. 3378-3384
    • Sylvester, J.T.1    Karnati, S.K.2    Yu, Z.3    Morrison, M.4    Firkins, J.L.5
  • 44
    • 0035374841 scopus 로고    scopus 로고
    • Diet-dependent shifts in the bacterial population of the rumen revealed with real-time PCR
    • Tajima, K., Aminov, R.I., Nagamine, T., Matsui, H., Nakamura, M. Benno, Y. (2001) Diet-dependent shifts in the bacterial population of the rumen revealed with real-time PCR. Appl Environ Microbiol 67, 2766 2774.
    • (2001) Appl Environ Microbiol , vol.67 , pp. 2766-2774
    • Tajima, K.1    Aminov, R.I.2    Nagamine, T.3    Matsui, H.4    Nakamura, M.5    Benno, Y.6
  • 45
    • 0020032328 scopus 로고
    • Use of Congo red-polysaccharide interactions in enumeration and characterization of cellulolytic bacteria from the bovine rumen
    • Teather, R.M. Wood, P.J. (1982) Use of Congo red-polysaccharide interactions in enumeration and characterization of cellulolytic bacteria from the bovine rumen. Appl Environ Microbiol 43, 777 780.
    • (1982) Appl Environ Microbiol , vol.43 , pp. 777-780
    • Teather, R.M.1    Wood, P.J.2
  • 46
    • 0032617899 scopus 로고    scopus 로고
    • Effect of diet on populations of three species of ruminal cellulolytic bacteria in lactating dairy cows
    • Weimer, P.J., Waghorn, G.C., Odt, C.L. Mertens, D.R. (1999) Effect of diet on populations of three species of ruminal cellulolytic bacteria in lactating dairy cows. J Dairy Sci 82, 122 134.
    • (1999) J Dairy Sci , vol.82 , pp. 122-134
    • Weimer, P.J.1    Waghorn, G.C.2    Odt, C.L.3    Mertens, D.R.4
  • 47
    • 0026198531 scopus 로고
    • Effects of the inclusion of yeast culture (Saccharomyces plus growth medium) in the diet of dairy cows on milk yield and forage degradation and fermentation patterns in the rumen of steers
    • Williams, P.E.V., Tait, C.A.G., Innes, C.A.G. Newbold, C.J. (1991) Effects of the inclusion of yeast culture (Saccharomyces plus growth medium) in the diet of dairy cows on milk yield and forage degradation and fermentation patterns in the rumen of steers. J Anim Sci 69, 3016 3026.
    • (1991) J Anim Sci , vol.69 , pp. 3016-3026
    • Williams, P.E.V.1    Tait, C.A.G.2    Innes, C.A.G.3    Newbold, C.J.4
  • 48
    • 2442510059 scopus 로고    scopus 로고
    • Improved extraction of PCR-quality community DNA from digesta and fecal samples
    • Yu, Z. Morrison, M. (2004) Improved extraction of PCR-quality community DNA from digesta and fecal samples. Biotechniques 36, 808 812.
    • (2004) Biotechniques , vol.36 , pp. 808-812
    • Yu, Z.1    Morrison, M.2


* 이 정보는 Elsevier사의 SCOPUS DB에서 KISTI가 분석하여 추출한 것입니다.