A rapid dual staining procedure for the quantitative discrimination of prion amyloid from tissues reveals how interactions between amyloid and lipids in tissue homogenates may hinder the detection of prions

Journal of Microbiological Methods

Volumn 77, Issue 1, 2009, Pages 90-97

  Herve R ^a   Collin, R ^a   Pinchin, H E ^a   Secker, T ^a   Keevil, C W ^a  

^a UNIVERSITY OF SOUTHAMPTON   (United Kingdom)

Author keywords

Homogenates; Instruments; Prion; Quantification

Indexed keywords

AMYLOID; LIPID; POLYSORBATE 20; PRION PROTEIN; PROTEIN; THIOFLAVINE;

ANIMAL TISSUE; ARTICLE; BRAIN; BRAIN HOMOGENATE; BRAIN INFECTION; CONTROLLED STUDY; DISINFECTION; ELUTION; EPIFLUORESCENCE MICROSCOPY; EPISCOPIC DIFFERENTIAL INTERFERENCE CONTRAST MICROSCOPY; INSTRUMENT DECONTAMINATION; INSTRUMENT STERILIZATION; NONHUMAN; OBSERVATIONAL STUDY; PHASE CONTRAST MICROSCOPY; PRION; PRIORITY JOURNAL; PROTEIN INTERACTION; QUANTITATIVE ANALYSIS; SENSITIVITY ANALYSIS; SIGNAL TRANSDUCTION; STAINING; SYPRO RUBY DUAL STAINING; TISSUE HOMOGENATE; VIRUS DETECTION; WESTERN BLOTTING;

AMYLOID; ANIMALS; BRAIN; LIPIDS; MICE; MICROSCOPY, INTERFERENCE; PRION DISEASES; PRIONS; SENSITIVITY AND SPECIFICITY; STAINING AND LABELING;

EID: 62349099657     PISSN: 01677012     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.mimet.2009.01.017     Document Type: Article

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