The sensitivity of approved Ninhydrin and Biuret tests in the assessment of protein contamination on surgical steel as an aid to prevent iatrogenic prion transmission

Journal of Hospital Infection

Volumn 64, Issue 3, 2006, Pages 288-292

  Lipscomb, I P ^a   Pinchin, H E ^a   Collin, R ^a   Harris, K ^a   Keevil, C W ^a  

^a UNIVERSITY OF SOUTHAMPTON   (United Kingdom)

Author keywords

Decontamination; Episcopic differential interference contrast microscopy; Prion disease; Protein tests; Soiling; Surgical instruments; SYPRO Ruby

Indexed keywords

FLUORESCENT DYE; NINHYDRIN; PRION PROTEIN; STAINLESS STEEL;

ANIMAL TISSUE; ARTICLE; BIURET TEST; BRAIN HOMOGENATE; COLORIMETRY; CONTROLLED STUDY; DISEASE TRANSMISSION; EPIFLUORESCENCE MICROSCOPY; FEMALE; INTERMETHOD COMPARISON; MOUSE; NINHYDRIN TEST; NONHUMAN; PHASE CONTRAST MICROSCOPY; PRION DISEASE; PROTEIN ANALYSIS; RISK REDUCTION; SENSITIVITY ANALYSIS; SURGICAL INSTRUMENT;

BIURET REACTION; DISEASE TRANSMISSION, PATIENT-TO-PROFESSIONAL; DISINFECTION; EQUIPMENT CONTAMINATION; EUROPIUM; FLUORESCENT DYES; HUMANS; INFECTIOUS DISEASE TRANSMISSION, PATIENT-TO-PROFESSIONAL; MICROSCOPY, INTERFERENCE; NINHYDRIN; PRION DISEASES; PRIONS; PROTEINS; SENSITIVITY AND SPECIFICITY; STAINLESS STEEL; SURGICAL INSTRUMENTS;

EID: 33749319496     PISSN: 01956701     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.jhin.2006.07.007     Document Type: Article

References (27)

1. NHS Estates. The decontamination of surgical instruments in the NHS in England - update report. 'A step change' (2005), Department of Health, London, UK
2. Department of Health. Decontamination of medical devices. Health Service Circular HSC 2000/032. London, UK: Department of Health; 2000.
3. Department of Health. Variant Creutzfeldt-Jakob disease (vCJD): minimising the risk of transmission. Health Service Circular HSC 1999/178. London, UK: Department of Health; 1999.
4. Department of Health. Controls assurance in infection control: decontamination of medical devices. Health Service Circular HSC 1999/179. London, UK: Department of Health; 1999.
5. The Parliamentary Office of Science and Technology. v-CJD in the future. London, UK: The United Kingdom Parliament; 171, 2002.
6. Department of Health. Hospital Episode Statistics England: financial year 2003-04 (2004), Department of Health, London, UK
7. NHS Estates. NHS decontamination project, background and expectations (2003), Department of Health, London, UK
8. NHS PASA. Paper on individual instrument and surgical tray identification (2004), Department of Health, London, UK
9. Quality task group. Cleaning (part 2) - validation of cleaning efficacy. Zentral Steril 10 (2002) 60-66
10. Quality task group. Verification of the performance of washer-disinfectors used for thermal disinfection. Zentral Steril 11 (2003) 295-296
11. ACDP/SEAC. Transmissible spongiform encephalopathy agents: safe working and the prevention of infection (2003), The Stationery Office, London, UK
12. Biotrace International. Central sterile service department and Pro-Tect: a new tool for validation. Application note. Bridgend, UK: Biotrace International; 2003.
13. Hansen D.B., and Joullie M.M. The development of novel ninhydrin analogues. Chem Soc Rev 34 (2005) 408-417
14. Schulz M.M., Wehner H.D., Reichert W., and Graw M. Ninhydrin-dyed latent fingerprints as a DNA source in a murder case. J Clin Forensic Med 11 (2004) 202-204
15. Worthington T., Adams N., and Elliott T.S. Diathermy pencils - a potential vector for the transmission of prions?. J Hosp Infect 47 (2001) 332-333
16. NHS Estates. Washer disinfectors. Hospital Technical Memorandum (HTM) 2030 (2001), Department of Health, London, UK
17. Betmouni S., and Perry V.H. The acute inflammatory response in CNS following injection of prion brain homogenate or normal brain homogenate. Neuropathol Appl Neurobiol 25 (1999) 20-28
18. Lipscomb I.P., Sihota A., Botham M., Harris K.L., and Keevil C.W. Rapid method for the sensitive detection of protein contamination on surgical instruments. J Hosp Infect 62 (2006) 141-148
19. Department of Health. Hospital episode statistics, England: financial year 2002-03 (2004), Department of Health, London, UK
20. NHS Estates. Comprehensive report: a review of the decontamination of surgical instruments in the NHS in England (2001), Department of Health, London, UK
21. Taylor D.M., Fernie K., McConnell I., and Steele P.J. Observations on thermostable subpopulations of the unconventional agents that cause transmissible degenerative encephalopathies. Vet Microbiol 64 (1998) 33-38
22. Taylor D.M. Inactivation of prions by physical and chemical means. J Hosp Infect 43 Suppl. (1999) S69-S76
23. Brown P., Rau E.H., Johnson B.K., Bacote A.E., Gibbs Jr. C.J., and Gajdusek D.C. New studies on the heat resistance of hamster-adapted scrapie agent: threshold survival after ashing at 600 degrees C suggests an inorganic template of replication. Proc Natl Acad Sci U S A 97 (2000) 3418-3421
24. DesCoteaux J.G., Poulin E.C., Julien M., and Guidoin R. Residual organic debris on processed surgical instruments. AORN J 62 (1995) 23-30
25. ACDP/SEAC. Transmissible spongiform encephalopathy agents: safe working and the prevention of infection (1998), The Stationery Office, London, UK
26. Sparrer H.E., Santoso A., Szoka Jr. F.C., and Weissman J.S. Evidence for the prion hypothesis: induction of the yeast [PSI+] factor by in vitro-converted Sup35 protein. Science 289 (2000) 595-599
27. Angelotti R., Wilson J.L., Litsky W., and Walter W.G. Comparative evaluation of the cotton swab and Rodac methods for the recovery of Bacillus subtilis spore contamination from stainless steel surfaces. Health Lab Sci 1 (1964) 289-296