Engineering lepidopteran insect cells for sialoglycoprotein production by genetic transformation with mammalian β1,4-galactosyltransferase and α2,6-sialyltransferase genes

Glycobiology

Volumn 11, Issue 1, 2001, Pages 1-9

  Hollister, J R ^a   Jarvis, D L ^a  

^a UNIVERSITY OF WYOMING   (United States)

Author keywords

Baculovirus expression system; Cell transformation; Genetic engineering; Insect cells; N glycosylation

Indexed keywords

BETA N ACETYLGLUCOSAMINYLGLYCOPEPTIDE BETA 1,4 GALACTOSYLTRANSFERASE; GLYCAN; GLYCOPROTEIN; GLYCOSYLTRANSFERASE; SIALOGLYCOPROTEIN; SIALYLTRANSFERASE;

ANIMAL CELL; ARTICLE; BACULOVIRUS; BIOSYNTHESIS; CELL LINE; CONTROLLED STUDY; ENZYME DEFICIENCY; GENE EXPRESSION; GENE EXPRESSION SYSTEM; GENETIC ENGINEERING; GENETIC TRANSFORMATION; HOST; INSECT; LEPIDOPTERA; MAMMAL CELL; NONHUMAN; PRIORITY JOURNAL; PROTEIN SYNTHESIS; SIALYLATION; VIRUS INFECTION;

ANIMALIA; INSECTA; LEPIDOPTERA; MAMMALIA; UNIDENTIFIED BACULOVIRUS;

EID: 0035093068     PISSN: 09596658     EISSN: None     Source Type: Journal    
DOI: 10.1093/glycob/11.1.1     Document Type: Article

References (42)

1. Processing of asparagine-linked oligosaccharides in insect cells. N-acetylglucosaminyltransferase I and II activities in cultured lepidopteran cells
2. Insect cells contain an usual, membrane-bound β-N-acetylglucosaminidase probably involved in the processing of protein N-glycans
3. A rapid, sensitive method for detection of alkaline phosphatase conjugated anti-antibody on western blot
4. Steps in the biosynthesis of mosquito cell membrane glycoproteins and the effects of tunicamycin
5. Modulation of circulatory residence of recombinant acetylcholinesterase through biochemical or genetic manipulation of sialylation levels
6. Oligosaccharide processing in the expression of human plasminogen cDNA by lepidopteran insect (Spodoptera frugiperda) cells
7. The role of carbohydrate chains of Galβ-1, 4-GlcNAcα2, 6-sialyltransferase for enzyme activity
8. SV40-transformed cells support the replication of early SV40 mutants
9. Monoclonal antibodies to baculovirus structural proteins: Determination of specificities by Western blot analysis
10. Genomic structure of murine β-1, 4-galactosyltransferase
11. Stable expression of mammalian β1, 4-galactosyltransferase extends the N-glycosylation pathway in insect cells
12. Constraints on the transport and glycosylation of recombinant IFN-gamma in Chinese hamster ovary and insect cells
13. N-linked glycosylation and sialylation of the acid-labile subunit. Role in complex formation with insulin-like growth factor (IGF)-binding protein-3 and the IGFs
14. Baculovirus expression vectors
15. Biochemical analysis of the N-glycosylation pathway in baculovirus-infected lepidopteran insect cells
16. Modifying the insect cell N-glycosylation pathway with immediate early baculovirus expression vectors
17. Use of early baculovirus promoters for continuous expression and efficient processing of foreign gene products in stably transformed lepidopteran cells
18. Immediate early baculovirus vectors for foreign gene expression in transformed or infected insect cells
19. Mutational analysis of the N-linked glycans on Autographa california nucleopolyhedrovirus gp64
20. Glycosylation of recombinant proteins: Problems and prospects
21. Isolation and characterization of an alpha 1, 2-mannosidase cDNA from the lepidopteran insect cell line Sf9
22. N-Glycan processing by a lepidopteran insect alpha1, 2-mannosidase
23. Assembly of asparagine-linked oligosaccharides
24. Insect cell hosts for baculovirus expression vectors contain endogenous exoglycosidase activity
25. Two naturally occurring α 2,6-sialyltransferase forms with a single amino acid change in the catalytic domain differ in their catalytic activity and proteolytic processing
26. Use of inhibitors to characterize intermediates in the processing of N-glycans synthesized by insect cells: A metabolic study with Sf9 cell line
27. Protein glycosylation in insects
28. The role of the protein glycosylation state in the control of cellular transport of the amyloid beta precursor protein
29. Role of asparagine-linked oligosaccharides in protein folding, membrane targeting and thyrotropin and autoantibody binding of the human thyrotropin receptor
30. A general approach to desalting oligosaccharides released from glycoproteins
31. Bovine galactosyltransferase: Identification of a clone by direct immunological screening of a cDNA expression library
32. Analysis of baculovirus genome with restriction endonucleases
33. Sialic acid contents of sindbis virus from vertebrate and mosquito cells. Equivalence of biological and immunological viral properties
34. A manual of methods for baculovirus vectors and insect cell culture procedures
35. Analysis of glycoprotein oligosaccharides using high-pH anion exchange chromatography
36. Glycosylation in lepidopteran insect cells: Identification of a β1,4-N-acetylgalactosaminyltransferase involved in the synthesis of complex-type oligosaccharide chains
37. The establishment of two insect cell lines from the insect Spodoptera frugiperda (Lepidoptera:Noctuidae)
38. Elongation of the N-glycans of fowl plaque virus hemagglutinin expressed in Spodoptera frugiperda (Sf9) cells by coexpression of human β1, 2-N-acetylglucosaminyltransferase I
39. Galactosyl and N-acetylgalactosaminyl homeostasis: A function for mammalian asialoglycoprotein receptors
40. Primary structure of β-galactoside α2, 6-sialyltransferase. Conversion of membrane-bound enzyme to soluble forms by cleavage of the NH2-terminal signal anchor
41. Stable replication of plasmids derived from Epstein-Barr virus in various mammalian cells