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From genetic diversity to metabolic unity: Studies on the biosynthesis of aurafurones and aurafuron-like structures in myxobacteria and streptomycetes
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Frank B, Wenzel SC, Bode HB, Scharfe M, Blöcker H, Müller R: From genetic diversity to metabolic unity: Studies on the biosynthesis of aurafurones and aurafuron-like structures in myxobacteria and streptomycetes. J Mol Biol (2007) 374(1):24-38.
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DKxanthene biosynthesis - understanding the basis for diversity-oriented synthesis in myxobacterial secondary metabolism
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Meiser P, Weissman KJ, Bode HB, Krug D, Dickschat JS, Sandmann A, Müller R: DKxanthene biosynthesis - understanding the basis for diversity-oriented synthesis in myxobacterial secondary metabolism. Chem Biol (2008) 15(8):771-781.
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Production of the highly antifungal isochromanone ajudazols in Chondromyces crocatus Cm c5: Biosynthetic machinery and cytochrome P450 tailoring modifications
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Buntin K, Rachid S, Scharfe M, Blöcker H, Weissman KJ, Müller R: Production of the highly antifungal isochromanone ajudazols in Chondromyces crocatus Cm c5: Biosynthetic machinery and cytochrome P450 tailoring modifications. Angew Chem Int Ed Engl (2008) 47(24):4595-4599.
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Unusual chemistry in the biosynthesis of the antibiotic chondrochlorens
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Rachid S, Scharfe M, Blöcker H, Weissman KJ, Müller R: Unusual chemistry in the biosynthesis of the antibiotic chondrochlorens. Chem Biol (2009) 16(1)70-81.
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Chem Biol
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Critical variations of conjugational DNA transfer into secondary metabolite multiproducing Sorangium cellulosum strains So ce12 and So ce56: Development of a mariner-based transposon mutagenesis system
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Kopp M, Irschik H, Gross F, Perlova O, Sandmann A, Gerth K, Müller R: Critical variations of conjugational DNA transfer into secondary metabolite multiproducing Sorangium cellulosum strains So ce12 and So ce56: Development of a mariner-based transposon mutagenesis system. J Biotechnol (2004) 107(1):29-40.
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48
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Molecular and biochemical studies of chondramide formation - highly cytotoxic natural products from Chondromyces crocatus Cm c5
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Rachid S, Krug D, Kochems I, Kunze B, Scharfe M, Blöcker H, Zabriski M, Müller R: Molecular and biochemical studies of chondramide formation - highly cytotoxic natural products from Chondromyces crocatus Cm c5. Chem Biol (2006) 13(6):667-681.
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Chem Biol
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49
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Discovery of additional members of the tyrosine aminomutase enzme family and mutational analysis of CmdF
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Krug D, Müller R: Discovery of additional members of the tyrosine aminomutase enzme family and mutational analysis of CmdF. ChemBioChem (2009):DOI 10.1002/cbic.200800748.
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ChemBioChem
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Krug, D.1
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50
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SorF, a glycosyltransferase with promiscuous donor substrate specificity in vitro
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Kopp M, Rupprath C, Irschik H, Bechthold A, Elling L, Müller R: SorF, a glycosyltransferase with promiscuous donor substrate specificity in vitro. ChemBioChem (2007) 8(7):813-819.
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ChemBioChem
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Kopp, M.1
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51
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Myxochelin biosynthesis: Direct evidence for two- and four-electron reduction of a carrier protein-bound thioester
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Li Y, Weissman KJ, Müller R: Myxochelin biosynthesis: Direct evidence for two- and four-electron reduction of a carrier protein-bound thioester. J Am Chem Soc (2008) 130:7554-7555.
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J Am Chem Soc
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Goldman BS, Nierman WC, Kaiser D, Slater SC, Durkin AS, Eisen J, Ronning CM, Barbazuk WB, Blanchard M, Field C, Halling C et al: Evolution of sensory complexity recorded in a myxobacterial genome. Proc Natl Acad Sci USA (2006) 103(41):15200-15205. • The complete sequence of the model strain Myxococcus xanthus DK1622 is reported, representing the first myxobacterial genome sequence. Theories about the evolutionary origins of the large bacterial chromosome (9.1 Mbp) are discussed.
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Goldman BS, Nierman WC, Kaiser D, Slater SC, Durkin AS, Eisen J, Ronning CM, Barbazuk WB, Blanchard M, Field C, Halling C et al: Evolution of sensory complexity recorded in a myxobacterial genome. Proc Natl Acad Sci USA (2006) 103(41):15200-15205. • The complete sequence of the model strain Myxococcus xanthus DK1622 is reported, representing the first myxobacterial genome sequence. Theories about the evolutionary origins of the large bacterial chromosome (9.1 Mbp) are discussed.
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53
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Complete genome sequence of the myxobacterium Sorangium cellulosum
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The genome sequence of S cellulosum So ce56, containing the largest bacterial chromosome known to date 13.0 Mbp, is presented and compared to the M xanthus genome, ••
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Schneiker S, Perlova O, Kaiser O, Gerth K, Alici A, Altmeyer MO, Bartels D, Bekel T, Beyer S, Bode E, Bode HB et al: Complete genome sequence of the myxobacterium Sorangium cellulosum. Nat Biotechnol (2007) 25(11):1281-1289. •• The genome sequence of S cellulosum So ce56, containing the largest bacterial chromosome known to date (13.0 Mbp), is presented and compared to the M xanthus genome.
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Nat Biotechnol
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The genomes of Myxococcus xanthus and Stigmatella aurantiaca
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Ronning CM, Nierman WC: The genomes of Myxococcus xanthus and Stigmatella aurantiaca. In: Myxobacteria: Multicellularity and Differentiation. Whitworth D (Ed), ASM Press, Washington DC, USA (2007):285-298.
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The mosaic genome of Anaeromyxobacter dehalogenans strain 2CP-C suggests an aerobic common ancestor to the δ-proteobacteria
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Thomas SH, Wagner RD, Arakaki AK, Skolnick J, Kirby JR, Shimkets LJ, Sanford RA, Loffler FE: The mosaic genome of Anaeromyxobacter dehalogenans strain 2CP-C suggests an aerobic common ancestor to the δ-proteobacteria. PLoS ONE (2008) 3(5):e2103.
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Mutasynthesis-derived myxalamids and origin of the isobutyryl-CoA starter unit of myxalamid B
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Bode HB, Meiser P, Klefisch T, Cortina NS, Krug D, Göhring A, Schwär G, Mahmud T, Elnakady YA, Müller R: Mutasynthesis-derived myxalamids and origin of the isobutyryl-CoA starter unit of myxalamid B. ChemBioChem (2007) 8(17):2139-2144.
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The unique DKxanthene secondary metabolite family from the myxobacterium Myxococcus xanthus is required for developmental sporulation
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Meiser P, Bode HB, Müller R: The unique DKxanthene secondary metabolite family from the myxobacterium Myxococcus xanthus is required for developmental sporulation. Proc Natl Acad Sci USA (2006) 103(50):19128-19133.
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Schley C, Altmeyer MO, Swart R, Müller R, Huber CG: Proteome analysis of Myxococcus xanthus by off-line two-dimensional chromatographic separation using monolithic poly-(styrene-divinylbenzene) columns combined with ion-trap tandem mass spectrometry. J Proteome Res (2006) 5(10):2760-2768. • The proteome of the model strain Myxococcus xanthus DK1622 is characterized, with a focus on the identification of high-molecular-weight polyketide synthases and non-ribosomal peptide synthetases. Intriguingly, this study shows that most of these megasynthetases are expressed under the tested experimental conditions.
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Schley C, Altmeyer MO, Swart R, Müller R, Huber CG: Proteome analysis of Myxococcus xanthus by off-line two-dimensional chromatographic separation using monolithic poly-(styrene-divinylbenzene) columns combined with ion-trap tandem mass spectrometry. J Proteome Res (2006) 5(10):2760-2768. • The proteome of the model strain Myxococcus xanthus DK1622 is characterized, with a focus on the identification of high-molecular-weight polyketide synthases and non-ribosomal peptide synthetases. Intriguingly, this study shows that most of these megasynthetases are expressed under the tested experimental conditions.
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Discovering the hidden secondary metabolome of Myxococcus xanthus: A study of intraspecific diversity
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Krug D, Zurek G, Revermann O, Vos M, Velicer GJ, Müller R: Discovering the hidden secondary metabolome of Myxococcus xanthus: A study of intraspecific diversity. Appl Environ Microbiol (2008) 74(10):3058-3068.
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Krug D, Zurek G, Schneider B, Garcia R, Müller R: Efficient mining of myxobacterial metabolite profiles enabled by liquid-chromatography - electrospray ionization - time-of-flight mass spectrometry and compound-based principal component analysis. Anal Chim Acta (2008) 624:97-106. •• Modern mass spectrometry-coupled liquid chromatography techniques are combined with statistical analysis tools to analyze bacterial metabolite profiles. The developed method allows the efficient mining of secondary metabolomes for the presence of potentially novel natural products.
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Krug D, Zurek G, Schneider B, Garcia R, Müller R: Efficient mining of myxobacterial metabolite profiles enabled by liquid-chromatography - electrospray ionization - time-of-flight mass spectrometry and compound-based principal component analysis. Anal Chim Acta (2008) 624:97-106. •• Modern mass spectrometry-coupled liquid chromatography techniques are combined with statistical analysis tools to analyze bacterial metabolite profiles. The developed method allows the efficient mining of secondary metabolomes for the presence of potentially novel natural products.
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Moderately thermophilic myxobacteria: Novel potential for production of natural products
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Gerth K, Müller R: Moderately thermophilic myxobacteria: Novel potential for production of natural products. Environ Microbiol (2005) 7(6):874-880.
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Garcia RO, Reichenbach H, Ring MW, Müller R: Phaselicystaceae, fam. nov., Phaselicystis flava, gen. nov., sp. nov., an arachidonic acid-containing soil myxobacterium. Int J Syst Evol Microbiol (2009): in press.
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Garcia RO, Reichenbach H, Ring MW, Müller R: Phaselicystaceae, fam. nov., Phaselicystis flava, gen. nov., sp. nov., an arachidonic acid-containing soil myxobacterium. Int J Syst Evol Microbiol (2009): in press.
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Recent developments towards the heterologous expression of complex bacterial natural product biosynthetic pathways
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Discusses different approaches for the heterologous production of natural products with particular emphasis on polyketides and non-ribosomally biosynthesized peptide compounds, •
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Wenzel SC, Müller R: Recent developments towards the heterologous expression of complex bacterial natural product biosynthetic pathways. Curr Opin Biotechnol (2005) 16(6):594-606. • Discusses different approaches for the heterologous production of natural products with particular emphasis on polyketides and non-ribosomally biosynthesized peptide compounds.
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Fu J, Wenzel SC, Perlova O, Gross F, Tang Z, Yin Y, Stewart AF, Müller R, Zhang Y: Efficient transfer of two large secondary metabolite pathways into heterologous hosts by transposition. Nucleic Acids Res (2008) 36(17):e113. •• A transposon-based approach for the delivery of large natural product biosynthetic pathways was established, as exemplified by the transfer of two myxobacterial biosynthetic gene clusters into different host organisms. In this study, the largest transposition yet reported for any system was achieved.
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Fu J, Wenzel SC, Perlova O, Gross F, Tang Z, Yin Y, Stewart AF, Müller R, Zhang Y: Efficient transfer of two large secondary metabolite pathways into heterologous hosts by transposition. Nucleic Acids Res (2008) 36(17):e113. •• A transposon-based approach for the delivery of large natural product biosynthetic pathways was established, as exemplified by the transfer of two myxobacterial biosynthetic gene clusters into different host organisms. In this study, the largest transposition yet reported for any system was achieved.
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Yin J, Straight PD, Hrvatin S, Dorrestein PC, Bumpus SB, Jao C, Kelleher NL, Kolter R, Walsh CT: Genome-wide high-throughput mining of natural-product biosynthetic gene clusters by phage display. Chem Biol (2007) 14(3):303-312. • Describes a phage-display strategy for the rapid and unambiguous identification of genes encoding polyketide synthases and non-ribosomal peptide synthetases. This method may provide an efficient tool for the cloning of secondary metabolite gene clusters from individual bacterial genomes and multigenome environmental DNA.
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Yin J, Straight PD, Hrvatin S, Dorrestein PC, Bumpus SB, Jao C, Kelleher NL, Kolter R, Walsh CT: Genome-wide high-throughput mining of natural-product biosynthetic gene clusters by phage display. Chem Biol (2007) 14(3):303-312. • Describes a phage-display strategy for the rapid and unambiguous identification of genes encoding polyketide synthases and non-ribosomal peptide synthetases. This method may provide an efficient tool for the cloning of secondary metabolite gene clusters from individual bacterial genomes and multigenome environmental DNA.
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