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Volumn 12, Issue 2, 2009, Pages 220-230

The biosynthetic potential of myxobacteria and their impact in drug discovery

Author keywords

Biosynthetic potential; Myxobacteria; Myxococcus; Natural products; Non ribosomal peptide; Polyketide; Sorangium

Indexed keywords

ADENOSINE TRIPHOSPHATASE; ALTHIOMYCIN; ARGYRIN A; CAROTENOID; CERAMIDE; CEREBROSIDE; CHLOROTONIL A; CHONDRAMIDE; CORALLOPYRONIN; CRUENTAREN A; DISORAZOL; EPOTHILONE DERIVATIVE; LEUPYRRIN; MIURAENAMIDE A; MYXOPYRONIN; PEDEIN A; PEPTIDE SYNTHASE; PHOXALONE; POLYKETIDE SYNTHASE; PYRROLNITRIN; RIPOSTATIN; RNA POLYMERASE; SAFRAMYCIN; SORANGIADENOSINE; SORAPHINOL C; STEROID; THUGGACIN; TUBULYSIN; TUBULYSIN D; UNCLASSIFIED DRUG; UNINDEXED DRUG;

EID: 62149106396     PISSN: 13676733     EISSN: None     Source Type: Journal    
DOI: None     Document Type: Review
Times cited : (93)

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    • The unique DKxanthene secondary metabolite family from the myxobacterium Myxococcus xanthus is required for developmental sporulation
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    • Schley C, Altmeyer MO, Swart R, Müller R, Huber CG: Proteome analysis of Myxococcus xanthus by off-line two-dimensional chromatographic separation using monolithic poly-(styrene-divinylbenzene) columns combined with ion-trap tandem mass spectrometry. J Proteome Res (2006) 5(10):2760-2768. • The proteome of the model strain Myxococcus xanthus DK1622 is characterized, with a focus on the identification of high-molecular-weight polyketide synthases and non-ribosomal peptide synthetases. Intriguingly, this study shows that most of these megasynthetases are expressed under the tested experimental conditions.
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    • Wenzel SC, Müller R: Recent developments towards the heterologous expression of complex bacterial natural product biosynthetic pathways. Curr Opin Biotechnol (2005) 16(6):594-606. • Discusses different approaches for the heterologous production of natural products with particular emphasis on polyketides and non-ribosomally biosynthesized peptide compounds.
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    • Fu J, Wenzel SC, Perlova O, Gross F, Tang Z, Yin Y, Stewart AF, Müller R, Zhang Y: Efficient transfer of two large secondary metabolite pathways into heterologous hosts by transposition. Nucleic Acids Res (2008) 36(17):e113. •• A transposon-based approach for the delivery of large natural product biosynthetic pathways was established, as exemplified by the transfer of two myxobacterial biosynthetic gene clusters into different host organisms. In this study, the largest transposition yet reported for any system was achieved.
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    • Yin J, Straight PD, Hrvatin S, Dorrestein PC, Bumpus SB, Jao C, Kelleher NL, Kolter R, Walsh CT: Genome-wide high-throughput mining of natural-product biosynthetic gene clusters by phage display. Chem Biol (2007) 14(3):303-312. • Describes a phage-display strategy for the rapid and unambiguous identification of genes encoding polyketide synthases and non-ribosomal peptide synthetases. This method may provide an efficient tool for the cloning of secondary metabolite gene clusters from individual bacterial genomes and multigenome environmental DNA.


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