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ITC experiments cannot be performed at higher concentrations because of the high viscosity of the solutions, and FTIR experiments cannot be performed at lower concentrations because of insufficient sensitivity
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ITC experiments cannot be performed at higher concentrations because of the high viscosity of the solutions, and FTIR experiments cannot be performed at lower concentrations because of insufficient sensitivity.
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Self-association of ureas in general and bis-urea EHUT in particular have been shown to be highly cooperative: K2 <
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2 < -ΔH.
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In a previous publication, the experimental transition between filaments and free monomers was somewhat arbitrarily chosen as the midpoint of the ITC heat-flow jump.6 However, if we define the critical concentration (c*) as the point where the free monomer concentration is equal to the total filament concentration, then calculations show that c * is closer to twice the midpoint of the heat-flow jump Figures S4 and S5 in the Supporting Information
-
* is closer to twice the midpoint of the heat-flow jump (Figures S4 and S5 in the Supporting Information).
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