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Volumn 131, Issue 3, 2009, Pages 1006-1014

Detecting protein-ligand binding on supported bilayers by local pH modulation

Author keywords

[No Author keywords available]

Indexed keywords

ACIDIC PH VALUES; ANTIBODY MOLECULES; BI-LAYER; BINDING INTERACTION; CHOLERA TOXIN B SUBUNIT; CONFIDENCE LEVELS; DYE MOLECULE; EQUILIBRIUM DISSOCIATION CONSTANT; HIGHLY SENSITIVE; IMAGING MODES; LIGAND BINDING; LIMIT OF DETECTION; PH SENSITIVE; PHOSPHOCHOLINE; PROTEIN BINDS; SIMULTANEOUS MEASUREMENT; SOLID INTERFACES; SUPPORTED PHOSPHOLIPID BILAYERS; TOTAL INTERNAL REFLECTION FLUORESCENCE MICROSCOPY;

EID: 61749094532     PISSN: 00027863     EISSN: None     Source Type: Journal    
DOI: 10.1021/ja804542p     Document Type: Article
Times cited : (48)

References (49)
  • 36
    • 67849122654 scopus 로고    scopus 로고
    • It should be noted that the IgG molecules did not noticeably aggregate as the pH was tuned between 4 and 10.2. In fact, no changes in the activity of antibiotin IgG were observed in the range of pH 4.0-10.2 (Supporting Information, This is to be expected as these proteins do not possess a single isoelectric point (pI) as the population is polyclonal
    • It should be noted that the IgG molecules did not noticeably aggregate as the pH was tuned between 4 and 10.2. In fact, no changes in the activity of antibiotin IgG were observed in the range of pH 4.0-10.2 (Supporting Information). This is to be expected as these proteins do not possess a single isoelectric point (pI) as the population is polyclonal.
  • 37
    • 84869568763 scopus 로고    scopus 로고
    • The apparent shift of ∼0.35 pH units corresponds to the shift in the midpoint of the titration curve for Figure 5b with respect to Figure 3b as determined by a sigmoidal fit to the data.
    • The apparent shift of ∼0.35 pH units corresponds to the shift in the midpoint of the titration curve for Figure 5b with respect to Figure 3b as determined by a sigmoidal fit to the data.
  • 40
    • 0003672412 scopus 로고    scopus 로고
    • 6th ed, John Wiley & Sons, Inc, New York
    • Christian, G. D. Analytical Chemistry, 6th ed.; John Wiley & Sons, Inc.: New York, 2004.
    • (2004) Analytical Chemistry
    • Christian, G.D.1
  • 45


* 이 정보는 Elsevier사의 SCOPUS DB에서 KISTI가 분석하여 추출한 것입니다.