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Volumn 302, Issue 5653, 2003, Pages 2094-2097

Biomolecular Interactions at Phospholipid-Decorated Surfaces of Liquid Crystals

Author keywords

[No Author keywords available]

Indexed keywords

CRYSTAL ORIENTATION; ENZYMES; LIQUID CRYSTALS; PHASE COMPOSITION; PROTEINS; STEREOCHEMISTRY;

EID: 0346362487     PISSN: 00368075     EISSN: None     Source Type: Journal    
DOI: 10.1126/science.1091749     Document Type: Article
Times cited : (607)

References (34)
  • 12
    • 0346430565 scopus 로고    scopus 로고
    • note
    • Previous studies have reported the use of LCs to image proteins bound to solid surfaces (13).
  • 14
    • 0345799472 scopus 로고    scopus 로고
    • note
    • Materials and methods are available as supporting material on Science Online,
  • 15
    • 0347691470 scopus 로고    scopus 로고
    • note
    • The temporal characteristics (∼0.5 to 2 hours) of the process of transfer of L-DLPC revealed by the LC are similar to previous studies of the fusion of vesicles with hydrophobic surfaces (17, 32).
  • 18
    • 0345799473 scopus 로고    scopus 로고
    • note
    • Whereas L-DLPC within vesicles is fluid and mobile at room temperature and thus readily transfers to form monolayers at hydrophobic interfaces, D-DPPC does not. By using fluorescence microscopy, we confirmed that the area[density of D-DPPC transferred to the aqueous-LC interface from vesicles was only ∼70% of that observed for L-DLPC. However, by using mixed micelles formed from DTAB and D-DPPC, we demonstrated that it was possible to prepare LC interfaces laden with D-DPPC with area[densities similar to the L-DLPC interfaces prepared from vesicles (33). D-DPPC transferred under these conditions also caused a perpendicular orientation of the LC at the aqueous-LC interface, thus establishing a general procedure for assembling lipids at the surface of LCs.
  • 20
    • 0347060772 scopus 로고    scopus 로고
    • note
    • 2/s (21), consistent with measurements of the diffusivities of lipids at a variety of interfaces, including biological membranes (17). Fluorescence imaging also revealed compartmentalization of the lipid between grid squares, which we have exploited to form patterned arrays of lipids at these interfaces (stability > 1 week).
  • 21
    • 0347060773 scopus 로고    scopus 로고
    • note
    • 0.5], where x is position, t is time, erf is the error function, and the diffusivity, D, was the only fitting parameter.
  • 22
    • 0347691471 scopus 로고    scopus 로고
    • note
    • 2 is found in the venom of a variety of animals (including snakes) and facilitates the passage of toxins across cell membranes.
  • 29
    • 0345799470 scopus 로고    scopus 로고
    • note
    • 2/s].
  • 34
    • 0345799471 scopus 로고    scopus 로고
    • note
    • Supported by NSF, NIH, and the U.S. Office of Naval Research.


* 이 정보는 Elsevier사의 SCOPUS DB에서 KISTI가 분석하여 추출한 것입니다.