Copper(II)-Alizarin Red S complex as an efficient chemiluminescent probe for the detection of human serum proteins after polyacrylamide gel electrophoresis

Journal of Proteome Research

Volumn 7, Issue 12, 2008, Pages 5075-5081

  Wang, Zhenzhen ^a   Liu, Xia ^a   Baeyens, Willy R G ^b   Delanghe, Joris R ^c   Ouyang, Jin ^a  

^a BEIJING NORMAL UNIVERSITY   (China)

^b GHENT UNIVERSITY   (Belgium)

^c GHENT UNIVERSITY HOSPITAL   (Belgium)

Author keywords

CBB staining; Chemiluminescence imaging; Copper(II) Alizarin Red S complex; Human serum proteins; Probe

Indexed keywords

ALIZARIN RED S; CUPRIC ION; HYDROGEN PEROXIDE; LUMINOL; PLASMA PROTEIN; POLYACRYLAMIDE GEL; REAGENT;

ANALYTIC METHOD; ARTICLE; CATALYSIS; CHEMOLUMINESCENCE; CONCENTRATION RESPONSE; HUMAN; METAL BINDING; MOLECULAR PROBE; NORMAL HUMAN; POLYACRYLAMIDE GEL ELECTROPHORESIS; PRIORITY JOURNAL; PROTEIN ANALYSIS; PROTEIN BINDING; REACTION ANALYSIS; SENSITIVITY ANALYSIS;

ANTHRAQUINONES; BIOCHEMISTRY; BLOOD PROTEINS; CATALYSIS; CHEMILUMINESCENT MEASUREMENTS; COPPER; EDETIC ACID; ELECTROPHORESIS, POLYACRYLAMIDE GEL; HUMANS; IONS; MODELS, CHEMICAL; PROTEINS; PROTEOMICS; SENSITIVITY AND SPECIFICITY; ULTRAVIOLET RAYS;

EID: 61349084270     PISSN: 15353893     EISSN: None     Source Type: Journal    
DOI: 10.1021/pr800365n     Document Type: Article

References (35)

1. Candiano, G.; Bruschi, M.; Musante, L.; Santucci, L.; Ghiggeri, G. M.; Carnemolla, B.; Orecchia, P.; Zardi, L.; Righetti, P. G. Blue silver: a very sensitive colloidal coomassie G-250 staining for proteome analysis. Electrophoresis 2004, 25 (9), 1327-1333.
2. Meyer, T. S.; Lamberts, B. L. Use of coomassie brilliant blue R250 for the electrophoresis of microgram quantities of parotid saliva proteins on acrylamide-gel strips. Biochim. Biophys. Acta 1965, 107 (1), 144-145.
3. Switzer, R. C.; Merril, C. R.; Shifrin, S. A highly sensitive silver stain for detecting proteins and peptides in polyacrylamide gels. Anal. Biochem. 1979, 98 (1), 231-237.
4. Winkler, C.; Denker, K.; Wortelkamp, S.; Sickmann, A. Silver- and Coomassie-staining protocols: detection limits and compatibility with ESI MS. Electrophoresis 2005, 28 (12), 2095-2099.
5. Steinberg, T. H.; Jones, L. J.; Haugland, R. P.; Singer, V. L. SYPRO Orange and SYPRO Red Protein gel stains: one-step fluorescent staining of denaturing gels for detection of nanogram levels of protein. Anal. Biochem. 1996, 239 (2), 223-237.
6. Steinberg, T. H.; Haugland, R. P.; Singer, V. L. Applications of SYPRO Orange and SYPRO Red Protein gel stains. Anal. Biochem. 1996, 239 (2), 238-245.
7. Steinberg, T. H.; Lauber, W. M.; Berggren, K.; Kemper, C.; Yue, S.; Patton, W. F. Fluorescence detection of proteins in sodium dodecyl sulfate-polyacrylamide gels using environmentally benign, non-fixative, saline solution. Electrophoresis 2000, 21 (3), 497-508.
8. Steinberg, T. H.; Chernokaiskaya, E.; Berggren, K.; Lopez, M. F.; Diwu, Z.; Haugland, R. P.; Patton, W. F. Ultrasensitive fluorescence protein detection in isoelectric focusing gels using a ruthenium metal chelate stain. Electrophoresis 2000, 21 (3), 486-496.
9. Smejkal, G. B.; Snajdar, R. M.; Hoff, H. F. Visualization of unstained protein bands on polyvinylidene fluoride membranes rehydrated in Tween 20. Biotechniques 1996, 21 (2), 232-233.
10. Smejkal, G. B.; Hoff, H. F. Cholesterol-specific probe for lipoproteins immobilized on nitrocellulose membranes. Biotechniques 1994, 16 (1), 68-70.
11. Smejkal, G. B.; Hoff, H. F. Co-localization of molecular mass marker proteins on Western blots. Biotechniques 1993, 15 (5), 796-798.
12. Zhang, X. H.; Ouyang, J.; Baeyens, W. R. G.; Delanghe, J. R.; Dai, Z. X.; Shen, S. H.; Huang, G. M. Direct chemiluminescent imaging detection of serum proteins in polyacrylamide gels. Anal. Chim. Acta 2003, 497 (1-2), 83-92.
13. Huang, G. M.; Ouyang, J.; Delanghe, J. R.; Baeyens, W. R. G.; Dai, Z. X. Chemiluminescent image detection of haptoglobin phenotyping after polyacrylamide gel electrophoresis. Anal. Chem. 2004, 76 (11), 2997-3004.
14. Shen, S. H.; Zhai, S. D.; Ouyang, J.; Zhang, X. H.; Zhang, H. Y. Direct chemiluminescent imaging method used to detect HbA-0, HbA-1, HbA-2 and HbF in human erythrocyte separated by polyacrylamide gel electrophoresis. Acta Chim. Sinica 2004, 62 (14), 1327-1332.
15. Chen, H. Y.; Zhao, H. P.; Huang, L. Y.; Baeyens, W. R. G.; Delanghe, J. R.; He, D. C.; Ouyang, J. Direct chemiluminescent imaging detection of Cu/Zn-superoxidase dismutase, glutathione peroxidase, carbonic anhydrase-III, and catalase in rat liver cytosol separated by native porous gradient polyacrylamide gel electrophoresis. Electrophoresis 2005, 26 (22), 4260-4269.
16. Tao, Q.; Wang, Z. Z.; Zhao, H. P.; Baeyens, W. R. G.; Delanghe, J. R.; Huang, L. Y.; Ouyang, J.; He, D. C.; Zhang, X. H. Direct chemiluminescent imaging detection of human serum proteins in two-dimensional polyacrylamide gel electrophoresis. Proteomics 2007, 7 (19), 3481-3490.
17. + probe for chemiluminescent imaging detection of proteins after polyacrylamide gel electrophoresis. Proteomics 2007, 7 (15), 2511-2521.
18. Mozdzan, M.; Szemraj, J.; Rysz, J.; Stolarek, R. A.; Nowak, D. Antioxidant activity of spermine and spermidine re-evaluated with oxidizing systems involving iron and copper ions. Int. J. Biochem. Cell Biol. 2006, 38 (1), 69-81.
19. Hopf, L. L.; Nieman, T. A. Effect of iron(II), cobalt(II), copper(II), and manganese(II) on the chemiluminescence of luminol in the absence of hydrogen peroxide. Anal. Chem. 1983, 55, 1080-1083.
20. Gorus, F.; Schram, E. Applications of bio- and chemiluminescence in the clinical laboratory. Clin. Chem. 1979, 25 (4), 512-519.
21. Whitehead, T. P.; Kricka, L. J.; Carter, T. J. N.; Thorpe, G. H. G. Analytical luminescence: its potential in the clinical laboratory. Clin. Chem. 1979, 25 (9), 1531-1546.
22. Uza, T.; Sasaki, S. A new copper(II) complex as an efficient catalyst of luminol chemiluminescence. Org. Lett. 2007, 9 (21), 4383-4386.
23. Jin, L. T.; Choi, J. K. Usefulness of visible dyes for the staining of protein or DNA in electrophoresis. Electrophoresis 2004, 25 (15), 2429-2438.
24. Hu, Q. L.; Wang, C. F.; Li, Q. M.; Zhang, Y. H.; Cao, X. Y.; Liu, S. C. Spectral characteristic of the reaction of copper(II)-Alizarin red S complex with protein and its applications. J. Instrum. Anal. 2005, 24 (6), 74-77.
25. Manabe, T.; Mizuma, H.; Watanabe, K. A nondenaturing protein map of human plasma proteins correlated with a denaturing polypeptide map combining techniques of micro two-dimensional gel electrophoresis. Electrophoresis 1999, 20 (4-5), 830-835.
26. Manabe, T.; Jin, Y.; Tani, O. Assignment of human plasma polypeptides on a nondenaturing 2-D gel using MALDI-MS and PMF and comparisons with the results of intact protein mapping. Electrophoresis 2007, 28 (5), 843-863.
27. Zhong, H.; Li, N.; Zhao, F.; Li, K. Determination of proteins with Alizarin Red S by Rayleigh light scattering technique. Talanta 2004, 62 (1), 37-42.
28. Wu, X. H.; Miao, J. G.; Miao, Y. Q.; Chen, J. R. Study on the Interaction of Hemoglobin and Cu(II)-ARS Complex. Spectrosc. Spec. Anal. 2007, 27 (6), 1168-1171.
29. Seltz, W. R.; Hercules, D. M. Chemiluminescence and Bioluminescence, Cormier, M. J., Hercules, D. M.; Lee, J., Eds.; Plenum Press: New York, 1973; pp 427-449.
30. Lundgren, J. S.; Salins, L. L. E.; Kaneva, I.; Daunert, S. A dynamical investigation of acrylodan-labeled mutant phosphate binding protein. Anal. Chem. 1999, 71 (3), 589-595.
31. Tian, J. N.; Liu, J. Q.; He, W. Y.; Hu, Z. D.; Yao, X. J.; Chen, X. G. Probing the binding of scutellarin to human serum albumin by circular dichroism, fluorescence spectroscopy, FTIR, and molecular modeling method. Biomacromolecules 2004, 5 (5), 1956-1961.
32. Hua, J.; Khin, M. M.; Zheng, P.; Yeoh, K.; Ng, H.; Ho, B. Serum IgG response to differentiated antigens of Helicobacter pylori. World J. Gastroenterol. 1998, 4 (3), 249-251.
33. Korstanje, R.; DiPetrillo, K. Unraveling the genetics of chronic kidney disease using animal models. Am. J. Physiol. Renal Physiol. 2004, 287, 347-352.
34. Williams, J.; Evans, R. W.; Moreton, K. The iron-binding properties of hen ovotransferrin. Biochem. J. 1978, 173, 535-542.
35. Ascenzi, P.; Bocedi, A.; Notari, S.; Menegatti, E.; Fasano, M. Heme impairs allosterically drug binding to human serum albumin Sudlow's site I. Biochem. Biophys. Res. Commun. 2005, 34 (2), 481-486.