Fluorescence detection of proteins in sodium dodecyl sulfate- polyacrylamide gels using environmentally benign, nonfixative, saline solution

Electrophoresis

Volumn 21, Issue 3, 2000, Pages 497-508

  Steinberg, Thomas H ^a   Lauber, Wendy M ^b   Berggren, Kiera ^a   Kemper, Courtenay ^a   Yue, Stephen ^a   Patton, Wayne F ^a  

^a MOLECULAR PROBES INC   (United States)

^b MONSANTO COMPANY   (United States)

Author keywords

Electrophoresis; Environment; Fluorescence; Mass spectrometry; Phosphate buffered saline; Protein stain; Zymography

Indexed keywords

AMINO ACIDS; ELECTROPHORESIS; ESTERS; GELS; MASS SPECTROMETRY; PROTEINS; SILVER COMPOUNDS; SODIUM CHLORIDE; SODIUM DODECYL SULFATE; SULFUR COMPOUNDS;

ENVIRONMENT; ENVIRONMENTALLY BENIGN; FLUORESCENCE DETECTION; LYSINE RESIDUES; PHOSPHATE-BUFFERED SALINES; POLYACRYLAMIDE GELS; PROTEIN STAIN; SALINE SOLUTIONS; SODIUM DODECYL SULPHATE; ZYMOGRAPHY;

FLUORESCENCE;

1 NAPHTHYL ACETATE; ANAZOLENE SODIUM; BETA GLUCURONIDASE; DODECYL SULFATE SODIUM; ESTERASE; FAST BLUE; FLUORESCENT DYE; PEPTIDE FRAGMENT; POLYACRYLAMIDE GEL; SILVER NITRATE; SODIUM CHLORIDE;

ALKYLATION; ARTICLE; ARTIFACT; ENZYME ACTIVITY; ESTERIFICATION; FLUORESCENCE; HYDROPHOBICITY; IMMUNOBLOTTING; MASS SPECTROMETRY; POLYACRYLAMIDE GEL ELECTROPHORESIS; PROTEIN DETERMINATION; PROTEIN MODIFICATION; SILVER STAINING; ULTRAVIOLET RADIATION; ZYMOGRAPHY;

EID: 0034002993     PISSN: 01730835     EISSN: None     Source Type: Journal    
DOI: 10.1002/(SICI)1522-2683(20000201)21:3<497::AID-ELPS497>3.0.CO;2-I     Document Type: Article

References (45)

1. Brush, M., The Scientist 1998, 12, 16-22.
2. Wirth, P., Romano, A., J. Chromatogr. A 1995, 698, 123-143.
3. Haebel, S., Albrecht, T., Sparbier, K., Walden, P., Korner, R., Steup, M., Electrophoresis 1998, 19, 679-686.
4. Rabilloud, T., Electrophoresis 1992, 13, 429-439.
5. Christiansen, J., Houer, G., Electrophoresis 1992, 13, 179-183.
6. Wilm, M., Shevchenko, A., Houthaeve, T., Breit, S., Schweigerer, L., Fotsis, T., Mann, M., Nature 1996, 379, 466-469.
7. Shevchenko, A., Jensen, O., Podtelejnikov, A., Sagliocco, F., Wilm, M., Vorm, O., Mortensen, P., Shevchenko, A., Boucherie, H., Mann, M., Proc. Natl. Acad. Sci. USA 1996, 93, 14440-14445.
8. Scheler, C., Lamer, S., Pan, Z., Li, X., Salnikow, J., Jungblut, P., Electrophoresis 1998, 19, 918-927.
9. Singer, V. L., Steinberg, T. H., Jones, L. J., Malekzadeh, N., Haugland, R. P., J. NIH Res. 1995, 7, 82.
10. Steinberg, T. H., Jones, L. J., Haugland, R. P., Singer, V. L., Anal. Biochem. 1996, 239, 223-227.
11. Steinberg, T. H., Haugland, R. P., Singer, V. L., Anal. Biochem. 1996, 239, 238-245.
12. Steinberg, T. H., White, H. M., Singer, V. L., Anal. Biochem. 1997, 248, 168-172.
13. Laemmli, U. K., Nature 1970, 227, 680-685.
14. Patton, W., Lopez, W., Barry, P., Skea, W., BioTechniques 1992, 12, 580-585.
15. Patton, W., Pluskal, M., Skea, W., Buecker, J., Lopez, M., Zimmerman, R., Belanger, L., Hatch, P., BioTechniques 1990, 8, 518-527.
16. Patton, W., J. Chromatogr. A 1995, 698, 55-87.
17. Tuma, R., Beaudet, M., Jin, X., Jones, L., Cheung, C., Yue, S., Singer, V., Anal. Biochem. 1999, 268, 278-288.
18. Towbin, H., Staehlin, T., Gordon, J., Proc. Natl. Acad. Sci. USA 1979, 76, 4350-4354.
19. Zhou, M., Upson, R., Diwu, Z., Haugland, R., J. Biochem. Biophys. Methods 1996, 33, 197-205.
20. Lauber, W., Carroll, J., Duffin, K., in: Savage, L. (Ed.), Proteomics: Integrating Protein Based Tools and Applications for Drug Discovery, International Business Communications, Southborough, MA 1998, pp. 385-400.
21. Dahlberg, A., Dingman, C., Peacock, A., J. Mol. Biol. 1969, 41, 139-147.
22. Green, M., Pastewka, J., Peacock, A., Anal. Biochem. 1977, 56, 43-51.
23. Campbell, K., MacLennan, D., Jorgensen, A., J. Biol. Chem. 1983, 258, 11267-11273.
24. Wade, M., O'Conner, J., BioTechniques 1992, 12, 794-796.
25. Gee, H., Cowman, M., Anal. Biochem. 1994, 219, 278-287.
26. Neumann, C., Forman, D., Rothlein, J., Environ. Health Perspect. 1998, 106, 217-226.
27. Pamies, R., Sugar, D., Rives, L., Herold, A., Postgrad. Med. 1993, 93, 183-184.
28. Hunter, E., Rogers, E., Schmid, J., Richard, A., Teratology 1996, 54, 57-64.
29. Rotstein, J., Slaga, T., Cancer Lett. 1988, 42, 87-90.
30. Sreeramulu, G., Singh, N., Electrophoresis 1995, 16, 362-365.
31. Nivinskas, H., Cole, K., BioTechniques 1996, 20, 380-385.
32. Neuhoff, V., Stamm, R., Pardowitz, I., Arold, N., Ehrhardt, W., Taube, D., Electrophoresis 1990, 11, 101-117.
33. Samso, M., Daban, J., Hansen, S., Jones, G., Eur. J. Biochem. 1995, 232, 818-824.
34. Shirahama, K., Tsujii, K., Takagi, T., J. Biochem. (Tokyo) 1974, 75, 309-319.
35. Tal, M., Silberstein, A., Nusser, E., J. Biol. Chem. 1985, 260, 9976-9980.
36. Rabilloud, T., Electrophoresis 1990, 11, 785-794.
37. Matulis, D., Lovrien, R., Biophys. J. 1998, 74, 422-429.
38. Matulis, D., Richardson, T., Lovrien, R., J. Mol. Recognit. 1996, 9, 433-443.
39. Conroy, M., Lovrien, R., J. Cryst. Growth 1992, 722, 213-222.
40. Allen, G., Biochem. J. 1974, 137, 575-578.
41. Shinagawa, S., Kameyama, K., Takagi, T., Biochim. Biophys. Acta 1993, 1161, 79-84.
42. Shoji, M., Kato, M., Hashizume, S., J. Chromatogr. A 1995, 698, 145-162.
43. Hunkapillar, M., Lujan, E., Ostrander, F., Hood, L., Methods. Enzymol. 1983, 91, 227-236.
44. Patterson, S., Aebersold, R., Electrophoresis 1995, 16, 1791-1814.
45. Loo, R., Dales, N., Andrews, P., Prof. Sci. 1994, 11, 1975-1983.