Ultrasensitive fluorescence protein detection in isoelectric focusing gels using a ruthenium metal chelate stain

Electrophoresis

Volumn 21, Issue 3, 2000, Pages 486-496

  Steinberg, Thomas H ^a   Chernokalskaya, Elena ^b   Berggren, Kiera ^a   Lopez, Mary F ^b   Diwu, Zhenjun ^a   Haugland, Richard P ^a   Patton, Wayne F ^a  

^a MOLECULAR PROBES INC   (United States)

^b Genomic Solutions   (United States)

Author keywords

Fluorescence; Isoelectric focusing; Noncovalent; Protein stain; Ruthenium

Indexed keywords

CHELATION; ELECTROPHORESIS; FOCUSING; GELS; MASS SPECTROMETRY; METAL COMPLEXES; PEPTIDES; RUTHENIUM COMPOUNDS; TENSILE STRENGTH;

DISTILLED WATER; FLUORESCENCE PROTEINS; ISOELECTRIC FOCUSING; METAL CHELATE; NONCOVALENT; PROTEIN DETECTION; PROTEIN GEL; PROTEIN STAIN; ULTRASENSITIVE; ULTRASENSITIVE FLUORESCENCE;

FLUORESCENCE;

AGAROSE; AMPHOLYTE; ANAZOLENE SODIUM; FLUORESCENT DYE; METAL CHELATE; N,N' METHYLENEBISACRYLAMIDE; PIPERAZINE DERIVATIVE; POLYACRYLAMIDE GEL; RUTHENIUM;

ARTICLE; CROSS LINKING; FLUORESCENCE; GEL ELECTROPHORESIS; ISOELECTRIC FOCUSING; MASS SPECTROMETRY; PROTEIN DETERMINATION; SILVER STAINING; TENSILE STRENGTH; ULTRAVIOLET B RADIATION;

EID: 0033999728     PISSN: 01730835     EISSN: None     Source Type: Journal    
DOI: 10.1002/(SICI)1522-2683(20000201)21:3<486::AID-ELPS486>3.0.CO;2-Q     Document Type: Article

References (36)

1. Righetti, P. G. Isoelectric Focusing: Theory, Methodology and Applications, Elsevier, New York, NY 1983.
2. Vesterberg, O., Electrophoresis 1998, 19, 1521-1524.
3. Kido, A., Kimura, Y. Oya, M., Electrophoresis 1995, 16, 1024-1026.
4. van den Berg, B., Electrophoresis 1998, 19, 1780-1787.
5. Marshall, T., Williams, K., Electrophoresis 1998, 19, 1752-1770.
6. Sondergaard, I., Jensen, K., Krath, B., Electrophoresis 1994, 15, 584-588.
7. Skarpeid, H., Kvaal, K., Hildrum, K., Electrophoresis 1998, 19, 3103-3109.
8. Ogorzalek Loo, R., Loo, J., Andrews, P., In: Link, A. J. (Ed.), Methods in Molecular Biology, Humana Press, Totowa, NJ 1999, pp. 473-485.
9. Ogorzalek Loo, R., Mitchell, C., Stevenson, T., Martin, S., Hines, W., Juhasz, P., Patterson, D., Peltier, J., Loo, J., Andrews P., Electrophoresis 1997, 18, 382-390.
10. Righetti, P. G., Immobilized pH gradients: Theory and methodology, Elsevier, New York, NY 1990.
11. Bjellqvist, B., Ek, K., Righetti, P., Gianazza, E., Görg, A., Postel, W., Westermeier, R., J. Biochem. Biophys. Methods 1982, 6, 317-339.
12. Görg, A., Postel, W., Günther, S., Electrophoresis 1988, 9, 531-546.
13. Righetti, P. G., Bossi, A., J. Chromatogr. B 1997, 699, 77-89.
14. Fawcett, J., Chrambach, A., Electrophoresis 1988, 9, 463-469.
15. Humpherey-Smith, I. Cordwell, S., Blackstock, W., Electrophoresis 1997, 18, 1217-1242.
16. Blomberg, A., Blomberg, L., Norbeck, J., Fey, S., Mose-Larsen, P., Larsen, M., Roepstorff, P., Degand, H., Boutry, M., Posch, A., Görg, A., Electrophoresis 1995, 18, 1935-1945.
17. Lopez M., Patton, W., Electrophoresis 1997, 18, 338-343.
18. Riley, R., Colemann, M. J. Lab. Clin. Med. 1968, 72, 714-720.
19. Hayes, M., Wellner, D., J. Biol. Chem. 1969, 244, 6636-6644.
20. Diezel, W., Kopperschläger, G., Hofmann, E., Anal. Biochem. 1972, 48, 617-620.
21. Allen, R., Masak, K., McAllister, P., Anal Biochem. 1980, 104, 494-498.
22. Roos, R., Lichter, M., J. Neurosci. Methods 1983, 8, 375-380.
23. Neuhoff, V., Arold, N., Taube, D., Ehrhardt, W., Electrophoresis 1988, 9, 255-262.
24. Pastestos, N., Fauth, M., Radola, B. J., Electrophoresis 1988, 9, 488-496.
25. Wirth, P., Romano, A. J. Chromatogr. A 1995, 698, 123-143.
26. Brush, M., The Scientist 1998, 12, 16-22.
27. Bermudez, A., Daban, J., Garcia, J., Mendez, E., BioTechniques 1994, 16, 621-624.
28. Patton, W., Lopez, M., Barry, P, Skea, W., BioTechniques 1992, 12, 580-585.
29. Patton, W., Pluskal, M., Skea, W., Buecker, J., Lopez, M., Zimmermann, R., Belanger, L., Hatch, P., Biotechniques, 1990, 8, 518-527.
30. Parker, K., Garrels, J., Hines, W., Butler, E., McKee, A., Patterson, D., Martin, S., Electrophoresis 1998, 19, 1920-1932.
31. Rabilloud, T., in: Link, A. J. (Ed.), Meth. Mol. Biol., Humana Press, Totowa, New Jersey, 1999, pp. 297-305.
32. Vesterberg, O., Acta Chem. Scand. 1969, 23, 2653-2666.
33. Pogacar, P., Jarecki, R., in: Allen, R., Maurer, H. (Ed.), Electrophoresis and Isoelectric Focusing in Polyacrylamide Gels, Walter de Gryter, Berlin 1974, pp. 153-158.
34. Grubhofer, N., Borja, C., in: Radola, B. J., Graesslin, D. (Eds.), Electrofocusing and Isotacophoresis, Walter de Gruyter, Berlin 1977, pp. 111-120.
35. Soderberg, J. Buckley, D., Hagstrom, G., Bergstrom, J., Prof. Biol. Fluids 1980, 27, 687-691.
36. Soderberg, J., US Patent 4,334,972, 1982.