Visualization of unstained protein bands on poly-vinylidene fluoride membranes rehydrated in Tween® 20

BioTechniques

Volumn 21, Issue 2, 1996, Pages 232-233

  Smejkal, Gary B ^a   Snajdar, Rudolf M ^a   Hoff, Henry F ^a  

^a LERNER RESEARCH INSTITUTE   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

ALBUMIN; ALPHA 2 MACROGLOBULIN; ERYTHROCYTE BAND 4.1 PROTEIN; LOW DENSITY LIPOPROTEIN; MEMBRANE PROTEIN; POLYSORBATE 20; POLYVINYLIDENE FLUORIDE;

ARTICLE; DNA PROBE; POLYACRYLAMIDE GEL ELECTROPHORESIS; PROTEIN ANALYSIS; PROTEIN BINDING; REHYDRATION;

EID: 0029758773     PISSN: 07366205     EISSN: None     Source Type: Journal    
DOI: 10.2144/96212bm13     Document Type: Article

References (7)

1. Kyhse-Andersen, J. 1984. Electroblotting of multiple gels: a simple apparatus without buffer tank for rapid transfer of proteins from polyacrylamide to nitrocellulose. J. Biochem. Biophys. Methods 10:203-209.
2. LeGendre, N. 1990. Immobilon-P transfer membrane: applications and utility in protein biochemical analysis. BioTechniques 9:788-805.
3. Mansfield, M.A. 1994. Using polyvinylidene fluoride membranes, p. 33-52. In B.S. Dunbar (Ed.), Protein Blotting: A Practical Approach. IRL Press, Oxford.
4. Reig, J.A. and D.C. Klein. 1988. Submicrogram quantities of unstained proteins are visualized on polyvinylidene difluoride membranes by transillumination. Appl. Theor. Electrophor. 1:59-60.
5. Smejkal, G.B. and H.F. Hoff. 1993. Co-localization of molecular mass marker proteins on Western blots. BioTechniques 75:796-798.
6. Smejkal, G.B. and H.F. Hoff. 1994. Filipin staining of lipoproteins in polyacrylamide gels: sensitivity and photobleaching of the fluorophore and its use in a double staining method. Electrophoresis 15:922-925.
7. Van Dam, A.P. 1994. Transfer and blocking conditions in immunoblotting, p. 79-80. In B.S. Dunbar (Ed.), Protein Blotting: A Practical Approach. IRL Press, Oxford.