Export of recombinant proteins in Escherichia coli using ABC transporter with an attached lipase ABC transporter recognition domain (LARD)

Microbial Cell Factories

Volumn 8, Issue , 2009, Pages

  Chung, Chan Woo ^a   You, Jinsun ^a   Kim, Kyeongyeon ^a   Moon, Yuseok ^b   Kim, Hoeon ^c   Ahn, Jung Hoon ^a,d  

^a Korea Science Academy   (South Korea)

^b Pusan National University School of Medicine   (South Korea)

^c Genexel Sein Inc   (South Korea)

^d Korea Advanced Institute of Science and Technology (KAIST)   (South Korea)

Author keywords

[No Author keywords available]

Indexed keywords

ABC TRANSPORTER; BLOOD CLOTTING FACTOR 10A; EPIDERMAL GROWTH FACTOR; GLYCINE; GREEN FLUORESCENT PROTEIN; HYBRID PROTEIN; LIPASE ABC TRANSPORTER; LIPASE ABC TRANSPORTER 0; LIPASE ABC TRANSPORTER 1; RECOMBINANT PROTEIN; TRIACYLGLYCEROL LIPASE; UNCLASSIFIED DRUG;

ARTICLE; BINDING SITE; CARBOXY TERMINAL SEQUENCE; ENZYME ACTIVITY; ESCHERICHIA COLI; NONHUMAN; PECTOBACTERIUM CHRYSANTHEMI; PROTEIN ANALYSIS; PROTEIN BINDING; PROTEIN DOMAIN; PROTEIN EXPRESSION; PROTEIN STRUCTURE; PSEUDOMONAS FLUORESCENS; SEQUENCE ANALYSIS; SIGNAL TRANSDUCTION; THERMOSTABILITY;

ERWINIA CHRYSANTHEMI; ESCHERICHIA COLI; NEGIBACTERIA; PSEUDOMONAS; PSEUDOMONAS AERUGINOSA; PSEUDOMONAS FLUORESCENS;

EID: 60949100697     PISSN: None     EISSN: 14752859     Source Type: Journal    
DOI: 10.1186/1475-2859-8-11     Document Type: Article

References (49)

1. Andersen C Channel-tunnels: Outer membrane components of type I secretion systems and multidrug efflux pumps of Gram-negative bacteria Reviews of physiology, biochemistry and pharmacology 2003, 147:122-165 12783268
2. Angkawidjaja C Kanaya S Family I.3 lipase: Bacterial lipases secreted by the type I secretion system Cell Mol Life Sci 2006, 63(23):2804-2817 17103114
3. Koronakis E Hughes C Milisav I Koronakis V Protein exporter function and in vitro ATPase activity are correlated in ABC-domain mutants of HlyB Mol Microbiol 1995, 16(1):87-96 7651140
4. Delepelaire P PrtD, the integral membrane ATP-binding cassette component of the Erwinia chrysanthemi metalloprotease secretion system, exhibits a secretion signal-regulated ATPase activity J Biol Chem 1994, 269(45):27952-27957 7961727
5. Schulein R Gentschev I Mollenkopf HJ Goebel W A topological model for the haemolysin translocator protein HlyD Mol Gen Genet 1992, 234(1):155-163 1495479
6. Zgurskaya HI Nikaido H Multidrug resistance mechanisms: Drug efflux across two membranes Mol Microbiol 2000, 37(2):219-225 10931319
7. Johnson JM Church GM Alignment and structure prediction of divergent protein families: Periplasmic and outer membrane proteins of bacterial efflux pumps J Mol Biol 1999, 287(3):695-715 10092468
8. Koronakis V Sharff A Koronakis E Luisi B Hughes C Crystal structure of the bacterial membrane protein TolC central to multidrug efflux and protein export Nature 2000, 405(6789):914-919 10879525
9. Kwon HJ Haruki M Morikawa M Omori K Kanaya S Role of repetitive nine-residue sequence motifs in secretion, enzymatic activity, and protein conformation of a family I.3 lipase J Biosci Bioeng 2002, 93(2):157-164 16233181
10. Welch RA RTX toxin structure and function: A story of numerous anomalies and few analogies in toxin biology Curr Top Microbiol Immunol 2001, 257:85-111 11417123
11. Binet R Letoffe S Ghigo JM Delepelaire P Wandersman C Protein secretion by Gram-negative bacterial ABC exporters - a review Gene 1997, 192(1):7-11 9224868
12. Ahn JH Pan JG Rhee JS Identification of the tliDEF ABC transporter specific for lipase in Pseudomonas fluorescens SIK W1 J Bacteriol 1999, 181(6):1847-1852 93584 10074078
13. Ahn JH Pan JG Rhee JS Homologous expression of the lipase and ABC transporter gene cluster, tliDEFA, enhances lipase secretion in Pseudomonas spp Appl Environ Microbiol 2001, 67(12):5506-5511 93336 11722899
14. Yang F Moss LG Phillips GN Jr The molecular structure of green fluorescent protein Nat Biotechnol 1996, 14(10):1246-1251 9631087
15. Ormo M Cubitt AB Kallio K Gross LA Tsien RY Remington SJ Crystal structure of the Aequorea victoria green fluorescent protein Science 1996, 273(5280):1392-1395 8703075
16. Mulec J Podlesek Z Mrak P Kopitar A Ihan A Zgur-Bertok D A cka-gfp transcriptional fusion reveals that the colicin K activity gene is induced in only 3 percent of the population J Bacteriol 2003, 185(2):654-659 145340 12511512
17. Zgurskaya HI Nikaido H Cross-linked complex between oligomeric periplasmic lipoprotein AcrA and the inner-membrane-associated multidrug efflux pump AcrB from Escherichia coli J Bacteriol 2000, 182(15):4264-4267 101937 10894736
18. Carpenter G Cohen S Epidermal growth factor J Biol Chem 1990, 265(14):7709-7712 2186024
19. Frankel AD Pabo CO Cellular uptake of the tat protein from human immunodeficiency virus Cell 1988, 55(6):1189-1193 2849510
20. Green M Loewenstein PM Autonomous functional domains of chemically synthesized human immunodeficiency virus tat trans-activator protein Cell 1988, 55(6):1179-1188 2849509
21. Kim D Jeon C Kim JH Kim MS Yoon CH Choi IS Kim SH Bae YS Cytoplasmic transduction peptide (CTP): New approach for the delivery of biomolecules into cytoplasm in vitro and in vivo Exp Cell Res 2006, 312(8):1277-1288 16466653
22. Guzzo J Duong F Wandersman C Murgier M Lazdunski A The secretion genes of Pseudomonas aeruginosa alkaline protease are functionally related to those of Erwinia chrysanthemi proteases and Escherichia coli alpha-haemolysin Mol Microbiol 1991, 5(2):447-453 1904127
23. Baumann U Wu S Flaherty KM McKay DB Three-dimensional structure of the alkaline protease of Pseudomonas aeruginosa: A two-domain protein with a calcium binding parallel beta roll motif Embo J 1993, 12(9):3357-3364 413609 8253063
24. Baumann U Crystal structure of the 50 kDa metallo protease from Serratia marcescens J Mol Biol 1994, 242(3):244-251 8089845
25. Hege T Baumann U Protease C of Erwinia chrysanthemi: The crystal structure and role of amino acids Y228 and E189 J Mol Biol 2001, 314(2):187-193 11718553
26. Arai R Ueda H Kitayama A Kamiya N Nagamune T Design of the linkers which effectively separate domains of a bifunctional fusion protein Protein Eng 2001, 14(8):529-532 11579220
27. Prescott M Nowakowski S Nagley P Devenish RJ The length of polypeptide linker affects the stability of green fluorescent protein fusion proteins Anal Biochem 1999, 273(2):305-307 10469502
28. Stanislawska-Sachadyn A Sachadyn P Ihle K Sydorczuk C Wiejacha K Kur J The construction of bifunctional fusion proteins consisting of MutS and GFP J Biotechnol 2006, 121(2):134-143 16157410
29. Stanley P Koronakis V Hughes C Acylation of Escherichia coli hemolysin: a unique protein lipidation mechanism underlying toxin function Microbiol Mol Biol Rev 1998, 62(2):309-333 98917 9618444
30. Felmlee T Welch RA Alterations of amino acid repeats in the Escherichia coli hemolysin affect cytolytic activity and secretion Proc Natl Acad Sci USA 1988, 85(14):5269-5273 281731 2839840
31. Ludwig A Jarchau T Benz R Goebel W The repeat domain of Escherichia coli haemolysin (HlyA) is responsible for its Ca2+-dependent binding to erythrocytes Mol Gen Genet 1988, 214(3):553-561 3063951
32. Ghigo JM Wandersman C A carboxyl-terminal four-amino acid motif is required for secretion of the metalloprotease PrtG through the Erwinia chrysanthemi protease secretion pathway J Biol Chem 1994, 269(12):8979-8985 8132636
33. Chervaux C Holland IB Random and directed mutagenesis to elucidate the functional importance of helix II and F-989 in the C-terminal secretion signal of Escherichia coli hemolysin J Bacteriol 1996, 178(4):1232-1236 177793 8576066
34. Holland IB Benabdelhak H Young J De Lima Pimenta A Schmitt L Blight MA Bacterial ABC transporters involved in protein translocation ABC proteins from bacterial to man London: Elsevier Science Holland IB, Cole SP, Kuchler K, Higgins CF 2003:209-241
35. Zhang F Yin Y Arrowsmith CH Ling V Secretion and circular dichroism analysis of the C-terminal signal peptides of HlyA and LktA Biochemistry 1995, 34(13):4193-4201 7703231
36. Izadi-Pruneyre N Wolff N Redeker V Wandersman C Delepierre M Lecroisey A NMR studies of the C-terminal secretion signal of the haem-binding protein, HasA Eur J Biochem 1999, 261(2):562-568 10215870
37. Wolff N Ghigo JM Delepelaire P Wandersman C Delepierre M C-terminal secretion signal of an Erwinia chrysanthemi protease secreted by a signal peptide-independent pathway: Proton NMR and CD conformational studies in membrane-mimetic environments Biochemistry 1994, 33(22):6792-6801 8204613
38. Rose T Sebo P Bellalou J Ladant D Interaction of calcium with Bordetella pertussis adenylate cyclase toxin. Characterization of multiple calcium-binding sites and calcium-induced conformational changes J Biol Chem 1995, 270(44):26370-26376 7592850
39. Ostolaza H Soloaga A Goni FM The binding of divalent cations to Escherichia coli alpha-haemolysin Eur J Biochem 1995, 228(1):39-44 7883008
40. Lilie H Haehnel W Rudolph R Baumann U Folding of a synthetic parallel beta-roll protein FEBS Lett 2000, 470(2):173-177 10734229
41. Angkawidjaja C You DJ Matsumura H Kuwahara K Koga Y Takano K Kanaya S Crystal structure of a family I.3 lipase from Pseudomonas sp. MIS38 in a closed conformation FEBS Lett 2007, 581(26):5060-5064 17923123
42. Meier R Drepper T Svensson V Jaeger KE Baumann U A calcium-gated lid and a large beta-roll sandwich are revealed by the crystal structure of extracellular lipase from Serratia marcescens J Biol Chem 2007, 282(43):31477-31483 17728256
43. Omori K Idei A Akatsuka H Serratia ATP-binding cassette protein exporter, Lip, recognizes a protein region upstream of the C terminus for specific secretion J Biol Chem 2001, 276(29):27111-27119 11335719
44. Palacios JL Zaror I Martinez P Uribe F Opazo P Socias T Gidekel M Venegas A Subset of hybrid eukaryotic proteins is exported by the type I secretion system of Erwinia chrysanthemi J Bacteriol 2001, 183(4):1346-1358 95009 11157948
45. Sambrook J Russell DW Molecular cloning: A laboratory manual N.Y.: Cold Spring Habor 3 2001.
46. Schwede T Kopp J Guex N Peitsch MC SWISS-MODEL: An automated protein homology-modeling server Nucleic Acids Res 2003, 31(13):3381-3385 168927 12824332
47. Fujiwara K Yasuno M Kitagawa T Novel preparation method of immunogen for hydrophobic hapten, enzyme immunoassay for daunomycin and adriamycin Journal of immunological methods 1981, 45(2):195-203 7026686
48. Brosius J Holy A Regulation of ribosomal RNA promoters with a synthetic lac operator Proc Natl Acad Sci USA 1984, 81(22):6929-6933 392049 6390428
49. Delepelaire P Wandersman C Protein secretion in gram-negative bacteria. The extracellular metalloprotease B from Erwinia chrysanthemi contains a C-terminal secretion signal analogous to that of Escherichia coli alpha-hemolysin J Biol Chem 1990, 265(28):17118-17125 2211614