The control of anchorage-dependent cell behavior within a hydrogel/microcarrier system in an osteogenic model

Biomaterials

Volumn 30, Issue 12, 2009, Pages 2259-2269

  Wang, Chunming ^a   Gong, Yihong ^a   Zhong, Yuan ^a   Yao, Yongchang ^a   Su, Kai ^a   Wang, Dong An ^a  

^a Nanyang Technological University   (Singapore)

Author keywords

Anchorage dependent cells; Apoptosis; Hydrogel; Integrin; Microcarrier; Osteogenesis

Indexed keywords

ANCHORAGE-DEPENDENT CELLS; APOPTOSIS; INTEGRIN; MICROCARRIER; OSTEOGENESIS;

ADHESION; ANCHORAGES (FOUNDATIONS); BONE; CELL CULTURE; CELL DEATH; GELATION; GELS; GLYCOPROTEINS; OSTEOBLASTS; TISSUE ENGINEERING;

HYDROGELS;

INTEGRIN; LIGAND;

APOPTOSIS; ARTICLE; CELL ADHESION; CELL ANCHORAGE; CELL DEATH; CELL FUNCTION; CELL SPREADING; CELL STRUCTURE; CELL SURVIVAL; CELL SUSPENSION; CONTROLLED STUDY; FETUS; FOCAL ADHESION; HUMAN; HUMAN CELL; HYDROGEL; IN VITRO STUDY; IN VIVO STUDY; MESENCHYMAL STEM CELL; MICROCARRIER CULTURE; OSTEOBLAST; PRIORITY JOURNAL;

APOPTOSIS; BIOLOGICAL MARKERS; CELL ADHESION; CELLS, CULTURED; HUMANS; HYDROGEL; INTEGRINS; MODELS, BIOLOGICAL; OSTEOBLASTS; OSTEOGENESIS; TRANSCRIPTION, GENETIC;

EID: 60849137197     PISSN: 01429612     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.biomaterials.2008.12.072     Document Type: Article

References (44)

1. Griffith L.G., and Naughton G. Tissue engineering: current challenges and expanding opportunities. Science 295 (2002) 1009-1014
2. Green D.W., Leveque I., Walsh D., Howard D., Yang X., Patridge K., et al. Biomineralized polysaccharide capsules for encapsulation, organization and delivery of human cell types and growth factors. Adv Funct Mater 15 (2005) 917-923
3. Drury J., and Mooney D.J. Hydrogels for tissue engineering: scaffold design variables and applications. Biomaterials 24 (2003) 4337-4351
4. Fedorovich N., Alblas J., de Wijn J., Hennink W., Verbout A., and Dhert W. Hydrogels as extracellular matrices for skeletal tissue engineering: state-of-the-art and novel application in organ printing. Tissue Eng 13 (2007) 1905-1925
5. Wang D.-A., Williams C.G., Li Q., Sharma B., and Elisseeff J.H. Synthesis and characterization of a novel degradable phosphate-containing hydrogel. Biomaterials 24 (2003) 3969-3980
6. Luo Y., and Schoichet M.S. A photolabile hydrogel for guided three-dimensional cell growth and migration. Nat Mater 3 (2004) 249-253
7. Benya P.D., and Shaffer J.D. Dedifferentiated chondrocytes reexpress the differentiated collagen phenotype when cultured in agarose gels. Cell 30 (1982) 215-224
8. Ruoslahti E., and Reed J.C. Anchorage dependence, integrins, and apoptosis. Cell 77 (1994) 477-478
9. Grigoriou V., Shapiro I., Cavalcanti-Adam E.A., Composto R.J., Ducheyne P., and Adams C.S. Apoptosis and survival of osteoblast-like cells are regulated by surface attachment. J Biol Chem 280 (2005) 1733-1739
10. Bahrami S., Stratmann U., Wiesmann H.-P., Mokrys K., Bruckner P., and Szuwart T. Periosteally derived osteoblast-like cells differentiate into chondrocytes in suspension culture in agarose. Anat Rec 259 (2000) 124-130
11. Nuttelman C.R., Tripodi M.C., and Anseth K.S. In vitro osteogenic differentiation of human mesenchymal stem cells photoencapsulated in PEG hydrogels. J Biomed Mater Res 68A 4 (2004) 773-782
12. Nuttelman C.R., Tripodi M.C., and Anseth K.S. Synthetic hydrogel niches that promote hMSC viability. Matrix Biol 24 (2005) 208-218
13. Hern D.L., and Hubbell J.A. Incorporation of adhesion peptides into non-adhesive hydrogels useful for tissue resurfacing. J Biomed Mater Res 39 (1998) 266-276
14. Wozniak M.A., Modzelewska K., Kwong L., and Keely P.J. Focal adhesion regulation of cell behavior. Biochim Biophys Acta 1692 (2004) 103-119
15. Wang D.-A., Williams C.G., Yang F., and Elisseeff J.H. Enhancing the tissue-biomaterial interface: tissue-initiated integration of biomaterials. Adv Funct Mater 14 (2004) 1152-1159
16. Malda J., and Frondoza C.G. Microcarriers in the engineering of cartilage and bone. Trends Biotechnol 24 (2006) 299-304
17. Wang C., Gong Y., Lin Y., Shen J., and Wang D.-A. A novel gellan gel-based microcarrier for anchorage-dependent cell delivery. Acta Biomater 4 (2008) 1226-1234
18. Harris S.A., Enger R.J., Riggs B.L., and Spelsberg T.C. Development and characterization of a conditionally immortalized human fetal osteoblastic cell line. J Bone Miner Res 10 (1995) 178-186
19. Li Z., Zhou Z., Saunders M.M., and Donahue H.J. Modulation of connexin43 alters expression of osteoblastic differentiation markers. Am J Physiol Cell Physiol 290 (2006) 1248-1255
20. Peter S.J., Liang C.R., Kim D.J., Widmer M.S., and Mikos A.G. Osteoblastic phenotype of rat marrow stromal cells cultured in the presence of dexamethasone, beta-glycerolphosphate, and l-ascorbic acid. J Cell Biochem 71 (1998) 55-62
21. Wang D.-A., Williams C.G., Yang F., Cher N., Lee H., and Elisseeff J.H. Bioresponsive phosphoester hydrogels for bone tissue engineering. Tissue Eng 11 (2005) 201-213
22. Zhu J.X., Liu M.Y., Kennedy R.H., and Liu S.J. TNF-a-induced impairment of mitochondrial integrity and apoptosis mediated by caspase-8 in adult ventricular myocytes. Cytokine 34 (2006) 96-105
23. Wang C., Hao J., Zhang F., Su K., and Wang D.-A. RNA extraction from polysaccharide-based cell-laden hydrogel scaffold. Anal Biochem 380 (2008) 333-334
24. Kozlova N.I., Morozevich G.E., Chubukina A.N., Shtil A.A., and Berman A.E. Expression of integrins, anchorage dependent apoptosis and invasiveness of multidrug resistant human breast carcinoma cells. EXCLI J 3 (2004) 68-81
25. Sussman L.K., Upalakalin J.N., Roberts M.J., Kocher O., and Benjamin L.F. Blood markers for vasculogenesis increase with tumor progression in patients with breast carcinoma. Cancer Biol Ther 2 (2003) 255-256
26. Dyson J.A., Genever P.G., Dalgarno K.W., and Wood D.J. Development of custom-built bone scaffolds using mesenchymal stem cells and apatite-wollastonite glass-ceramics. Tissue Eng 13 (2007) 2891-2901
27. Wang Y.H., Liu Y., Maye P., and Rowe D.W. Examination of mineralized nodule formation in living osteoblastic cultures using fluorescent dyes. Biotechnol Prog 22 (2006) 1697-1701
28. Lee J.Y., Choo J.E., Park H.J., Park J.B., Lee S.C., Jo I., et al. Injectable gel with synthetic collagen-binding peptide for enhanced osteogenesis in vitro and in vivo. Biochem Biophys Res Commun 357 (2007) 68-74
29. Richards P.J., Williams A.S., Goodfellow R.M., and Williams B.D. Liposomal clodronate eliminates synovial macrophages, reduces inflammation and ameliorates joint destruction in antigen-induced arthritis. Rheumatology (Oxford) 38 (1999) 818-825
30. Anselme K. Osteoblast adhesion on biomaterials. Biomaterials 21 (2000) 667-681
31. Ziegler W.H., Liddington R.C., and Critchley D.R. The structure and regulation of vinculin. Trends Cell Biol 16 (2006) 453-460
32. Huttenlocher A., Sandborg R.R., and Horwitz A.F. Adhesion in cell migration. Curr Opin Cell Biol 7 (1995) 697-706
33. Sodek J., and Cheifetz S. Molecular regulation of osteogenesis. In: Davies J.E. (Ed). Bone engineering (1999), Em Squared, Toronto, Canada [chapter 4]
34. Komori T. Runx2, a multifunctional transcription factor in skeletal development. J Cell Biochem 87 (2002) 1-8
35. Komori T. Regulation of skeletal development by the runx family of transcription factors. J Cell Biochem 95 (2005) 445-453
36. Weber L.M., Hayda K.N., Haskins K., and Anseth K.S. The effects of cell-matrix interactions on encapsulated beta-cell function within hydrogels functionalized with matrix-derived adhesive peptides. Biomaterials 28 (2007) 3004-3011
37. Stupack D.G., Puente X.S., Boutsaboualoy S., Storgard C.M., and Cheresh D.A. Apoptosis of adherent cells by recruitment of caspase-8 to unligated integrins. J Cell Biol 155 (2001) 459-470
38. Small J.V., Rottner K., Kaverina I., and Anderson K.I. Assembling an actin cytoskeleton for cell attachment and movement. Biochim Biophys Acta 1404 (1998) 271-281
39. Frisch S.M., and Ruoslahti E. Integrins and anoikis. Curr Opin Cell Biol 9 (1997) 701-706
40. Re F., Zanetti M., Sironi N., Polentarutti L., Lanfrancone E., Dejana E., et al. Inhibition of anchorage-dependent cell spreading triggers apoptosis in cultured human endothelial calls. J Cell Biol 127 (1994) 537-546
41. Chen C.S., Mrkisch M., Huang S., Whitesides G.M., and Ingber D. Geometric control of cell life and death. Science 276 (1997) 1425-1428
42. Shima M., Seino Y., Tanaka H., Kurose H., Ishida M., Yabuuchi H., et al. Microcarriers facilitate mineralization in MC3T3-E1 cells. Calcif Tissue Int 43 (1988) 19-25
43. Overstreet M., Sohrabi A., Polotsky A., Hungerford D.S., and Frondoza C.G. Collagen microcarrier spinner culture promotes osteoblast proliferation and synthesis of matrix proteins. In Vitro Cell Dev Biol Anim 39 (2003) 228-234
44. Anderson H.C., and Morris D.C. Mineralization. In: Mundy G.R., and Martin T.J. (Eds). Physiology and pharmacology of bone (1993), Springer, Berlin, Germany