Purification of Cadmium-Ion Binding Metallothionein-3 by Proteinase Digestion on Affinity Chromatographic Column

Chinese Journal of Chromatography

Volumn 24, Issue 3, 2006, Pages 279-283

  ZHENG, Weijuan ^a   YANG, Feng ^a   WU, Fang ^a   LU, Chun ^a   HUA, Zichun ^a  

^a NANJING UNIVERSITY   (China)

Author keywords

affinity chromatography; Factor Xa digestion on column; fusion expression; human metallothionein 3 (hMT 3); purification

Indexed keywords

CADMIUM; CADMIUM DERIVATIVE; CADMIUM SULFIDE; GLUTATHIONE TRANSFERASE; METALLOTHIONEIN; PEPTIDE; PEPTIDE HYDROLASE; SULFIDE;

AFFINITY CHROMATOGRAPHY; ARTICLE; GENETICS; HUMAN; METABOLISM; METHODOLOGY; POLYACRYLAMIDE GEL ELECTROPHORESIS;

CADMIUM; CADMIUM COMPOUNDS; CHROMATOGRAPHY, AFFINITY; ELECTROPHORESIS, POLYACRYLAMIDE GEL; GLUTATHIONE TRANSFERASE; HUMANS; METALLOTHIONEIN; PEPTIDE HYDROLASES; PEPTIDES; SULFIDES;

EID: 33745833078     PISSN: 18722059     EISSN: None     Source Type: Journal    
DOI: 10.1016/S1872-2059(06)60013-8     Document Type: Article

References (11)

1. Uchida Y., Takio K., Titani K., Ihara Y., and Tomonaga M. Neuron 7 2 (1991) 337
2. Palmiter R., Findley S., Whitmore T., and Durnam D. Proc Natl Acad Sci USA 89 14 (1992) 6333
3. Ji L., and Mo T. Bioinorganic Chemistry-An Introduction (1992), Zhongshan University Press, Guangzhou, China 311
4. Vallee B. Methods in Enzymology 205 (1991) 3
5. Wang S., Chang C., Chen C., Tam M., Shih Y., and Lin L. Gene 203 (1997) 189
6. Bogumil R., Faller P., Pountney D., and Vasak M. Eur J Biochem 238 3 (1996) 698
7. Shi Y., Du L., Zheng W., and Tang W. Biometals 15 (2002) 421
8. Zheng W., Wu F., Zhuang H., Lu C., Yang F., Ma W., and Hua Z. Preparative Biochemistry and Biotechnology 34 (2004) 265
9. Luo Z., and Hua Z. Biochemistry and Molecular Biology Interna-tional 46 3 (1998) 471
10. Smith D., and Johnson K. Gene 67 (1988) 31
11. Chen C., Zhuang H., Zheng W., and Hua Z. Chinese Journal of Chromatography 22 4 (2004) 456