Functional analysis of MCNA, a gene encoding a catalytic subunit of calcineurin, in the rice blast fungus Magnaporthe oryzae

Journal of Microbiology and Biotechnology

Volumn 19, Issue 1, 2009, Pages 11-16

  Choi, Jinhee ^a   Kim, Yangseon ^a   Lee, Yong Hwan ^a  

^a Seoul National University   (South Korea)

Author keywords

Appressorium; Calcineurin; Calmodulin; Magnaporthe oryzae; Protein phosphatase; Sense antisense

Indexed keywords

CALCIUM; CALMODULIN; COMPLEMENTARY RNA; FUNGAL PROTEIN; MAGNAPORTHE ORYZAE CALCINEURIN A PROTEIN; UNCLASSIFIED DRUG; CALCINEURIN; CYCLOSPORIN; FUNGAL DNA;

APPRESSORIUM; ARTICLE; ENZYME ACTIVITY; FUNCTIONAL GENOMICS; FUNGAL MORPHOLOGY; FUNGAL STRAIN; FUNGAL VIRULENCE; GENE EXPRESSION; MAGNAPORTHE; MAGNAPORTHE ORYZAE; MYCELIAL GROWTH; NONHUMAN; RICE BLAST; SACCHAROMYCES CEREVISIAE; DRUG ANTAGONISM; DRUG EFFECT; FUNGAL GENE; FUNGUS SPORE; GENETICS; MICROBIOLOGY; PATHOGENICITY; PH; PHYSIOLOGY; RICE; RNA INTERFERENCE; SIGNAL TRANSDUCTION;

FUNGI; MAGNAPORTHE GRISEA; MAGNAPORTHE ORYZAE; SACCHAROMYCES CEREVISIAE;

CALCINEURIN; CYCLOSPORINE; DNA, FUNGAL; GENE EXPRESSION; GENES, FUNGAL; HYDROGEN-ION CONCENTRATION; MAGNAPORTHE; ORYZA SATIVA; RNA INTERFERENCE; RNA, ANTISENSE; SIGNAL TRANSDUCTION; SPORES, FUNGAL;

EID: 60649118958     PISSN: 10177825     EISSN: 17388872     Source Type: Journal    
DOI: 10.4014/jmb.0804.268     Document Type: Article

References (41)

1. Adachi, K. and J. E. Hamer. 1998. Divergent cAMP signaling pathways regulate growth and pathogenesis in the rice blast fungus Magnaporthe grisea. Plant Cell 10: 1361-1374.
2. Bader, T., B. Bodendorfer, K. Schroppel, and J. Morschhauser. 2003. Calcineurin is essential for virulence in Candida albicans. Infect. Immun. 71: 5344-5354.
3. Bae, C. Y., S. Kim, W. B. Choi, and Y. H. Lee. 2007. Involvement of extracellular matrix and integrin-like proteins on conidial adhesion and appressorium differentiation in Magnaporthe oryzae. J. Microbiol. Biotechnol. 17: 1198-1203.
4. Bencina, M., M. Legisa, and N. D. Read. 2005. Cross-talk between cAMP and calcium signalling in Aspergillus niger. Mol. Microbiol. 56: 268-281.
5. Bok, J. W., T. Sone, L. B. Silverman-Gavrila, R. R. Lew, F. J. Bowring, D. E. Catcheside, and A. J. Griffiths. 2001. Structure and function analysis of the calcium-related gene spray in Neurospora crassa. Fungal Genet. Biol. 32: 145-158.
6. Choi, G. H., B. Chen, and D. L. Nuss. 1995. Virus-mediated or transgenic suppression of a G-protein alpha subunit and attenuation of fungal virulence. Proc. Natl. Acad. Sci. USA 92: 305-309.
7. Davis, K. R. and F. M. Ausubel. 1989. Characterization of elicitor-induced defense responses in suspension-cultured cells of Arabidopsis. Mol. Plant Microbe Interact. 2: 363-368.
8. Dickman, M. B. and O. Yarden. 1999. Serine/threonine protein kinases and phosphatases in filamentous fungi. Fungal Genet. Biol. 26: 99-117.
9. Fire, A., S. Xu, M. K. Montgomery, S. A. Kostas, S. E. Driver, and C. C. Mello. 1998. Potent and specific genetic interference by double-stranded RNA in Caenorhabditis elegans. Nature 391: 806-811.
10. Gilbert, R. D., A. M. Johnson, and R. A. Dean. 1996. Chemical signals responsible for appressorium formation in the rice blast fungus Magnaporthe grisea. Physiol. Molec. Plant Pathol. 48: 335-346.
11. Hamer, J. E. and N. J. Talbot. 1998. Infection-related development in the rice blast fungus Magnaporthe grisea. Curr. Opin. Microbiol. 1: 693-697.
12. Harel, A., S. Bercovich, and O. Yarden. 2006. Calcineurin is required for sclerotial development and pathogenicity of Sclerotinia sclerotiorum in an oxalic acid-independent manner. Mol. Plant Microbe Interact. 19: 682-693.
13. Juvvadi, P. R., M. Arioka, H. Nakajima, and K. Kitamoto. 2001. Cloning and sequence analysis of cnaA gene encoding the catalytic subunit of calcineurin from Aspergillus oryzae. FEMS Microbiol. Lett. 204: 169-174.
14. Juvvadi, P. R., Y. Kuroki, M. Arioka, H. Nakajima, and K. Kitamoto. 2003. Functional analysis of the calcineurin-encoding gene cnaA from Aspergillus oryzae: Evidence for its putative role in stress adaptation. Arch. Microbiol. 179: 416-422.
15. Kadotani, N., H. Nakayashiki, Y. Tosa, and S. Mayama. 2003. RNA silencing in the phytopathogenic fungus Magnaporthe oryzae. Mol. Plant Microbe Interact. 16: 769-776.
16. Kafadar, K. A. and M. S. Cyert. 2004. Integration of stress responses: Modulation of calcineurin signaling in Saccharomyces cerevisiae by protein kinase A. Eukaryot. Cell 3: 1147-1153.
17. Kim, S., I. P. Ahn, H. S. Rho, and Y. H. Lee. 2005. MHP1, a Magnaporthe grisea hydrophobin gene, is required for fungal development and plant colonization. Mol. Microbiol. 57: 1224-1237.
18. Lee, S. C. and Y. H. Lee. 1998. Calcium/calmodulin-dependent signaling for appressorium formation in the plant pathogenic fungus Magnaporthe grisea. Mol. Cells 8: 698-704.
19. Lee, Y.-H. and R. A. Dean. 1993. cAMP regulates infection structure formation in the plant pathogenic fungus Magnaporthe grisea. Plant Cell 5: 693-700.
20. Lee, Y.-H. and R. A. Dean. 1994. Hydrophobicity of contact surface induces appressorium formation in Magnaporthe grisea. FEMS Microbiol. Lett. 115: 71-75.
21. Leung, H., U. Lehtinen, R. Karjalainen, D. Skinner, P. Tooley, S. Leong, and A. Ellingboe. 1990. Transformation of the rice blast fungus Magnaporthe grisea to hygromycin B resistance. Curr. Genet. 17: 409-411.
22. Liu, S. and R. A. Dean. 1997. G protein alpha subunit genes control growth, development, and pathogenicity of Magnaporthe grisea. Mol. Plant Microbe Interact. 10: 1075-1086.
23. Liu, Z. M. and P. E. Kolattukudy. 1999. Early expression of the calmodulin gene, which precedes appressorium formation in Magnaporthe grisea, is inhibited by self-inhibitors and requires surface attachment. J. Bacteriol. 181: 3571-3577.
24. Mendoza, I., F. J. Quintero, R. A. Bressan, P. M. Hasegawa, and J. M. Pardo. 1996. Activated calcineurin confers high tolerance to ion stress and alters the budding pattern and cell morphology of yeast cells. J. Biol. Chem. 271: 23061-23067.
25. Mendoza, I., F. Rubio, A. Rodriguez-Navarro, and J. M. Pardo. 1994. The protein phosphatase calcineurin is essential for NaCl tolerance of Saccharomyces cerevisiae. J. Biol. Chem. 269: 8792-8796.
26. Moore, T. M., P. M. Chetham, J. J. Kelly, and T. Stevens. 1998. Signal transduction and regulation of lung endothelial cell permeability. Interaction between calcium and cAMP. Am. J. Physiol. 275: L203-L222.
27. Nakamura, T., Y. Liu, D. Hirata, H. Namba, S. Harada, T. Hirokawa, and T. Miyakawa. 1993. Protein phosphatase type 2B (calcineurin)-mediated, FK506-sensitive regulation of intracellular ions in yeast is an important determinant for adaptation to high salt stress conditions. EMBO J. 12: 4063-4071.
28. Odom, A., S. Muir, E. Lim, D. L. Toffaletti, J. Perfect, and J. Heitman. 1997. Calcineurin is required for virulence of Cryptococcus neoformans. EMBO J. 16: 2576-2589.
29. Ou, S. H. 1985. Rice Diseases. Commonwealth Mycological Institute, Kew, U.K.
30. Pallen, C. J. and J. H. Wang. 1983. Calmodulin-stimulated dephosphorylation of para-nitrophenyl phosphate and free phosphotyrosine by calcineurin. J. Biol. Chem. 258: 8550-8553.
31. 2+ gradient. Mol. Gen. Genet. 256: 104-114.
32. Rasmussen, C., C. Garen, S. Brining, R. L. Kincaid, R. L. Means, and A. R. Means. 1994. The calmodulin-dependent protein phosphatase catalytic subunit (calcineurin A) is an essential gene in Aspergillus nidulans. EMBO J. 13: 3917-3924.
33. Rusnak, F. and P. Mertz. 2000. Calcineurin: Form and function. Physiol. Rev. 80: 1483-1521.
34. Sambrook, J., E. F. Fritsch, and T. Maniatis. 1989. Molecular Cloning: A Laboratory Manual, Cold Spring Harbor Laboratory Press, NY, Cold Spring Harbor, NY.
35. Steinbach, W. J., R. A. Cramer, Jr., B. Z. Perfect, Y. G. Asfaw, T. C. Sauer, L. K. Najvar, et al. 2006. Calcineurin controls growth, morphology, and pathogenicity in Aspergillus fumigatus. Eukaryot. Cell 5: 1091-1103.
36. Talbot, N. J., D. J. Ebbole, and J. E. Hamer. 1993. Identification and characterization of MPG1, a gene involved in pathogenicity from the rice blast fungus Magnaporthe grisea. Plant Cell 5: 1575-1590.
37. Viaud, M., A. Brunet-Simon, Y. Brygoo, J. M. Pradier, and C. Levis. 2003. Cyclophilin A and calcineurin functions investigated by gene inactivation, cyclosporin A inhibition and cDNA arrays approaches in the phytopathogenic fungus Botrytis cinerea. Mol. Microbiol. 50: 1451-1465.
38. Viaud, M. C., P. V. Balhadere, and N. J. Talbot. 2002. A Magnaporthe grisea cyclophilin acts as a virulence determinant during plant infection. Plant Cell 14: 917-930.
39. Wesley, S. V., C. A. Helliwell, N. A. Smith, M. B. Wang, D. T. Rouse, Q. Liu, et al. 2001. Construct design for efficient, effective and high-throughput gene silencing in plants. Plant J. 27: 581-590.
40. Xu, J. R. and J. E. Hamer. 1996. MAP kinase and cAMP signaling regulate infection structure formation and pathogenic growth in the rice blast fungus Magnaporthe grisea. Genes Dev. 10: 2696-2706.
41. Yi, M. and Y. H. Lee. 2008. Identification of genes encoding heat shock protein 40 family and the functional characterization of two Hsp40s, MHF16, and MHF21, in Magnaporthe oryzae. Plant Pathol. J. 24: 131-142.