In situ 2D fluorometry and chemometric monitoring of mammalian cell cultures

Biotechnology and Bioengineering

Volumn 102, Issue 4, 2009, Pages 1098-1106

  Teixeira, Ana P ^a,b   Portugal, Carla A M ^b   Carinhas, Nuno ^a   Dias, João M L ^b   Crespo, João P ^a   Alves, Paula M ^a   Carrondo, M J T ^a   Oliveira, Rui ^b  

^a INSTITUTO DE BIOLOGIA EXPERIMENTAL E TECNOLÓGICA   (Portugal)

^b UNIVERSIDADE NOVA DE LISBOA   (Portugal)

Author keywords

2d fluorometry; Bioprocess monitoring; Chemometric methods; Mammalian cell cultures

Indexed keywords

2D FLUOROMETRY; ACCURATE ESTIMATIONS; BIOPROCESS; BIOPROCESS MONITORING; CALIBRATION METHODOLOGIES; CELL LINES; CHEMOMETRIC; CHEMOMETRIC METHODS; CHEMOMETRIC MODELING; CONCENTRATION OF; CULTURE TIME; FED BATCHES; FLUORESCENCE SIGNALS; FLUOROPHORS; HAMSTER KIDNEYS; IN-SITU; MAMMALIAN CELL CULTURES; NAD(P)H; ON-LINE MONITORING; OPERATION CONDITIONS; PARTIAL LEAST-SQUARES REGRESSIONS; PLS MODELS; PREDICTIVE CAPACITIES; PRINCIPAL COMPONENTS ANALYSIS; RECOMBINANT PROTEINS; SPECTRAL DATUM; TARGET STATE; TRAINING SETS; VALIDATION STRATEGIES; VIABLE CELLS; WAVELENGTH RANGES;

AMINES; AMINO ACIDS; ANIMAL CELL CULTURE; CELLS; CHROMATOGRAPHY; CONCENTRATION (PROCESS); CURVE FITTING; DYNAMICS; FLUORESCENCE; FLUOROMETERS; GLYCOPROTEINS; MAMMALS; ORGANIC ACIDS; PRINCIPAL COMPONENT ANALYSIS; PROTEINS;

CELL CULTURE;

AMINO ACID; GLYCOPROTEIN; HYBRID PROTEIN; IMMUNOGLOBULIN G1; INTERLEUKIN 2; RECOMBINANT PROTEIN; REDUCED NICOTINAMIDE ADENINE DINUCLEOTIDE PHOSPHATE; VITAMIN; IMMUNOGLOBULIN G;

ACCURACY; ANIMAL CELL; ARTICLE; BACTERIUM CULTURE; CALIBRATION; CELL DENSITY; CELL VIABILITY; CHEMOMETRICS; FED BATCH CULTURE; FLUORESCENCE; FLUOROMETRY; MAMMAL CELL; MONITORING; NONHUMAN; PARTIAL LEAST SQUARES REGRESSION; PRINCIPAL COMPONENT ANALYSIS; PROTEIN EXPRESSION; RELIABILITY; TWO DIMENSIONAL FLUOROMETRY; VALIDATION PROCESS; ANIMAL; BIOREACTOR; BIOSYNTHESIS; CELL LINE; CELL SURVIVAL; CULTURE TECHNIQUE; EVALUATION; GENETICS; HAMSTER; METHODOLOGY; REPRODUCIBILITY;

CRICETINAE; MAMMALIA;

ANIMALS; BIOREACTORS; CELL CULTURE TECHNIQUES; CELL LINE; CELL SURVIVAL; CRICETINAE; FLUOROMETRY; IMMUNOGLOBULIN G; INTERLEUKIN-2; RECOMBINANT FUSION PROTEINS; REPRODUCIBILITY OF RESULTS;

EID: 60549109858     PISSN: 00063592     EISSN: 10970290     Source Type: Journal    
DOI: 10.1002/bit.22125     Document Type: Article

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