An integrated strategy for the process development of a recombinant antibody-cytokine fusion protein expressed in BHK cells

Applied Microbiology and Biotechnology

Volumn 52, Issue 3, 1999, Pages 345-353

  Burger, C ^a   Carrondo, M J T ^b   Cruz, H ^b   Cuffe, M ^c   Dias, E ^b   Griffiths, J B ^d   Hayes, K ^d   Hauser, H ^e   Looby, D ^d   Mielke, C ^e   Moreira, J L ^b   Rieke, E ^a   Savage, A V ^c   Stacey, G N ^f   Welge, T ^a  

^a MERCK KGAA   (Germany)

^b INSTITUTO DE BIOLOGIA EXPERIMENTAL E TECNOLÓGICA   (Portugal)

^c NATIONAL UNIVERSITY OF IRELAND   (Ireland)

^d PUBLIC HEALTH ENGLAND   (United Kingdom)

^e HELMHOLTZ CENTRE FOR INFECTION RESEARCH   (Germany)

^f NATIONAL INSTITUTE FOR BIOLOGICAL STANDARDS AND CONTROL   (United Kingdom)

Author keywords

[No Author keywords available]

Indexed keywords

ANTINEOPLASTIC AGENT; HYBRID PROTEIN; RECOMBINANT ANTIBODY;

ANIMAL CELL; ANTINEOPLASTIC ACTIVITY; ARTICLE; BIOTECHNOLOGY; CELL CULTURE; CELL SECRETION; CELL STRAIN BHK; DRUG MECHANISM; DRUG SYNTHESIS; NONHUMAN;

ANIMALS; ANTINEOPLASTIC AGENTS; BIOREACTORS; CARBOHYDRATE SEQUENCE; CELL LINE; CHROMATOGRAPHY, HIGH PRESSURE LIQUID; CRICETINAE; CULTURE MEDIA, SERUM-FREE; DRUG SCREENING ASSAYS, ANTITUMOR; GENES; GENES, SYNTHETIC; GENETIC VECTORS; GLYCOSYLATION; IMMUNOGLOBULIN G; KIDNEY; MESOCRICETUS; MOLECULAR SEQUENCE DATA; OLIGOSACCHARIDES; PROTEIN PROCESSING, POST-TRANSLATIONAL; RECEPTOR, EPIDERMAL GROWTH FACTOR; RECOMBINANT FUSION PROTEINS; SELECTION (GENETICS); SPECTROMETRY, MASS, MATRIX-ASSISTED LASER DESORPTION-IONIZATION; TUMOR CELLS, CULTURED; TUMOR NECROSIS FACTOR-ALPHA;

ANIMALIA;

EID: 0032884613     PISSN: 01757598     EISSN: None     Source Type: Journal    
DOI: 10.1007/s002530051530     Document Type: Article

References (30)

1. Artlett P, Morelle C, Ausmeier M, Fitzek M, Hauser H (1988) Vectors for efficient expression in mammalian fibroblastoid, myeloid and lymphoid cells via transfection or infection. Gene 68: 213-219
2. Bebbington CR, Renner G, Thompson S, King D, Abrams D, Yarranton GT (1992) High level expression of a recombinant antibody from myeloma cells using a glutamine synthetase gene as an amplifiable selectable marker. Bio Technology 10: 169-175
3. Birch JR, Boraston H, Metcalfe H, Brown ME, Bebbington CR, Field RP (1994) Selecting and designing cell lines for improved physiological characteristics. Cytotechnology 15: 11-16
4. Church GM, Gilbert W (1984) Genomic sequencing. Proc Natl Acad Sci USA 81: 1991-1995
5. Cruz HJ, Moreira JL, Stacey GN, Dias EM, Hayes K, Looby D, Griffiths JB, Carrondo MJT (1998) Adaptation of BHK cells producing a recombinant protein to serum free media and protein free medium. Cytotechnology 26: 59-64
6. Cummings DA (1991) Glycosylation of recombinant therapeutics: control and functional implications. Glycobioloay 1: 115-135
7. Dirks W, Wirth M, Hauser H (1993) Dicistronic transcription units for gene expression in mammalian cells. Gene 128: 247-249
8. Gawlitzek M, Valley U, Nimtz M, Wagner R, Conradt HS (1995) Characterisation of changes in the glycosylation pattern of recombinant proteins from BHK-21 cells due to different culture conditions. J Biotechnol 42: 117-131
9. Geyer R, Geyer H (1993) Isolation and fractionation ot glycoprotein glycans in small amounts. In: Hounsell EF (ed) Glycoprotein analysis in biomedicine. Humana Press, Totowa, NJ, pp 131-142
10. Hakomori S-I (1964) A rapid permethylation of glycolipid and olygosaccharide catalysed by methylsulfinyl carbonion in demethylsulphoxide. J Biochem (Tokyo) 55: 205-208
11. Hoegen I von, Hofman U, Jaegle C-J, Strimatter W, Stadtmuller J, Matzkus (1993) Immunoconjugates. EP/24.12.93/EP 93120865
12. Huberty MC, Vath JE, Yu W, Martin SA (1993) Site specific carbohydrate identification m recombinant proteins using MALDI-TOF-MS. Anal Biochem 65: 2791-2800
13. Hunt SMN, Pak SCO, Bridges MW, Gray PP, Sleigh MJ (1997) Chinese hamster ovary cells produce sufficient recombinant insulin-like growth factor I to support the growth in serum-free medium. Cytotechnology 24: 55-64
14. Ito Y, Zhens J, Imanishi Y, Yonesawa K, Kasuga M (1996) Protein-free cell culture on artificial substrates with covalently immobilised insulin. Proc Natl Acad Sci USA 93: 3598-3601
15. Karreman S, Hauser H, Karreman C (1996) On the use of FLP recognition targets (FRTs) in the LTR of retroviruses for the construction of high producer cell lines. Nucleic Acids Res 24: 1616-1624
16. Kettleborough CA, Saldanha J, Heath VJ, Morrison CJ, Bendig MM (1991) Humanisation of a mouse monoclonal antibody by CDR-grafting: the importance of framework residues on loop conformation. Protein Eng 4: 773-783
17. Kratje RB, Reimann A, Hammer J, Wagner R (1994) Cultivation of recombinant baby hamster kidney cells in a fluidised bed bioreactor system with porous borosilicate glass. Biotechnol Prog 10: 410-420
18. Laemlli UK (1970) Cleavage of structural proteins during assembly of the head of bacteriophage T4. Nature 227: 680-685
19. Looby D, Griffiths JB (1988) Fixed bed porous glass sphere ('porosphere') bioreactors for animal cells. Cytotechnology 1: 339-346
20. Luchette C, Deer F, Gurnett K, Rosenthal J, Johnson E, Polito A, Cunningham M (1997) Isolation and characterisation of a CHO cell line expressing rhFSH in low protein and protein tree media. In: Carrondo MJT, Griffiths JB, Moreira JL (eds) Animal cell technology: from vaccines to genetic medicine. Kluwer, Dordrecht, pp 669-674
21. Moreira JL, Alves P, Aunins JG, Carrondo MJT (1995) Hydrodynamic effects on BHK cells grown as suspended natural aggregates. Biotechnol Bioeng 46: 351-360
22. Nimtz M, Martin W, Wray V, Kloppel KD, Augustin J, Conradt HS (1993) Structures of sialylated oligosaccharides of human erythropoietin expressed in recombinant BHK-21 cells. Eur J Biochem 213: 39-56
23. Patel TP, Parekh RB (1994) Release of oligosaccharides from alycoproteins by hydrazinolysis. In: Fukuda M, Kobata A (eds) Guide to techniques in glycobiology. Academic Press, San Diego, pp 57-66
24. Racher AJ, Griffiths JB (1993) Investigation of parameters affecting a fixed bed bioreactor process for recombinant cell lines. Cytotechnology 13: 125-131
25. Rodeck U, Herlyn M, Herlyn D, Molthoff C, Atkinson B, Varello M, Steplewski Z, Koprowski H (1987) Tumour growth modulation by a monoclonal antibody to the epidermal growth factor receptor: immunologically mediated and effector cell-independent effects. Cancer Res 47: 3692-3696
26. Savage AV (1997) Glycosylation: a post-translational modification. In: Hauser H, Wagner R (eds) Mammalian cell biotechnology in protein production. de Gruyter, Berlin, pp 233-276
27. Sugarman BJ, Aagarwal BB, Hass PE, Figari IS, Palladino MA Jr, Shepard HM (1985) Recombinant human tumor necrosis factor-alpha: effects on proliferation of normal and transformed cells in vitro. Science 230: 943-945
28. Tarentino AL, Plummer TH (1994) Enzymatic deglycosylation of asparagine-linked glycans: purification, properties and specificity of oligosaccharide-cleaving enzymes from Flavobacterium menigosepticum. In: Fukuda M. Kobata A (eds) Guide to techniques in glycobiology. Academic Press, San Diego, pp 44-57
29. 3 IGF-1 with insulin in the support of cell growth and recombinant protein expression in CHO cells. In: Spier RE, Griffiths JB, Berthold W (eds) Animal cell technology. Butterworth-Hienemann, London, pp 91-95
30. Weitzhandler M, Hardy MR, Co MS, Avdalovic N (1994) HPAEC-PAD carbohydrate analysis of different IgG preparations. J Pharm Sci 83: 1670-1675