Molecular characterization of Bt cauliflower with multiplex PCR and validation of endogenous reference gene in Brassicaceae family

Current Science

Volumn 95, Issue 12, 2008, Pages 1729-1731

  Randhawa, Gurinder Jit ^a   Chhabra, Rashmi ^a   Singh, Monika ^a  

^a NATIONAL BUREAU OF PLANT GENETIC RESOURCES   (India)

Author keywords

Brassica oleracea var. botrytis; Endogenous reference gene; Multiplex PCR; S locus receptor kinase gene

Indexed keywords

BOTRYTIS; BRASSICA OLERACEA VAR. BOTRYTIS; BRASSICA OLERACEA VAR. CAPITATA; BRASSICACEAE;

EID: 60349101998     PISSN: 00113891     EISSN: None     Source Type: Journal    
DOI: None     Document Type: Article

References (13)

1. Clive, J., Global review of commercialized transgenic crops. ISAAA Briefs. No. 13: Preview, ISAAA, Ithaca, NY, 2007.
2. Department of Biotechnology webpage: http://www.dbtindia.nic.in
3. ChangJie, X., Yang, L. and Chen, K., Development of a rapid, reliable and simple multiplex PCR assay for early detection of transgenic plant materials. Acta Physiol. Plant., 2005, 27, 283-288.
4. Forte, V. T., Pinto, A. D., Martino, C., Tantillo, G. M., Grasso, G. and Schena, F. P., A general multiplex-PCR assay for the general detection of genetically modified soya and maize. Food Control, 2005, 16, 535-539.
5. Singh, C. K., Ojha, A., Bhatanagar, R. K. and Kachru, D. N., Detection and characterization of recombinant DNA expressing vip3A-type insecticidal gene in GMOs-standard single, multiplex and construct-specific PCR assays. Anal. Bioanal. Chem., 2007, 390, 377-387.
6. Yang, L. et al., Qualitative and quantitative PCR methods for event-specific detection of genetically modified cotton Mon1445 and Mon531. Transgenic Res., 2005, 14, 817-831.
7. Wang, W. and Fang, T. J., Development of multiplex and quantitative PCR assay to detect genetically modified roundup ready soybean in foods. J. Food Drug Anal., 2005, 13, 132-138.
8. Wall, E., Lawrence, T., Green, M. and Rott, M., Detection and identification of transgenic virus resistant papaya and squash by multiplex PCR. Eur. Food Res. Technol., 2004, 219, 90-96.
9. Yang, L., Chen, J., Huang, C., Liu, Y., Jia, S., Pan, L. and Zhang, D., Validation of a cotton-specific gene, Sad1, used as an endogenous reference gene in qualitative and real-time quantitative PCR detection of transgenic cottons. Plant Cell Rep., 2005, 24, 237-245.
10. Estruch, J. J., Carozzi, N. B., Desai, N., Duck, N. B., Warren, G. W. and Koziel, M. G., Transgenic plant: An emerging approach to pest control. Nature Biotechnol., 1997, 15, 137-141.
11. Saghai-Maroof, M. A., Soliman, K. M., Jorgensen, R. A. and Allard, R. W., Ribosomal DNA spacer length polymorphism in barley, Mendelian inheritance, chromosomal location and population dynamics. Proc. Natl. Acad. Sci. USA, 1984, 81, 8014-8019.
12. Adang, M. J., Staver, M. J., Rocheleau, T. A., Leighton, J., Barker, R. F. and Thompson, D. V., Characterized full-length and truncated plasmid clones of the crystal protein of Bacillus thuringiensis subsp. kurstaki HD-73 and their toxicity to Manduca Sexta. Gene, 1985, 36, 289-300.
13. Pietsch, K., Waiblinger, U., Brodmann, P. and Wurz, A., Screeningverfahren zur identifizietung gentechnisch veranderter lebensmittel. Dtsch. Lebensm.-Rundsch., 1999, 2, 35-38.