Effects of systemic administration of ciliary neurotrophic factor on Bax and Bcl-2 proteins in the lumbar spinal cord of neonatal rats after sciatic nerve transection

Brazilian Journal of Medical and Biological Research

Volumn 41, Issue 11, 2008, Pages 1024-1028

  Rezende, A C S ^a   Vieira, A S ^a   Rogerio F ^a   Rezende, L F ^a   Boschero, A C ^a   Negro, A ^b   Langone, F ^a  

^a UNIVERSITY OF CAMPINAS   (Brazil)

^b UNIVERSITY OF PADOVA   (Italy)

Author keywords

Axotomy; Bax; Ciliary neurotrophic factor; Immunoprecipitation; Motoneuron; Neonatal rats

Indexed keywords

CILIARY NEUROTROPHIC FACTOR; MESSENGER RNA; PROTEIN BAX; PROTEIN BCL 2;

ANIMAL CELL; ANIMAL EXPERIMENT; ANIMAL MODEL; ANIMAL TISSUE; APOPTOSIS; ARTICLE; CELL COUNT; CELL SURVIVAL; CONTROLLED STUDY; DRUG EFFICACY; DRUG MECHANISM; DRUG PROTEIN BINDING; HISTOCHEMISTRY; IMMUNOPRECIPITATION; LUMBAR SPINAL CORD; MOLECULAR DYNAMICS; NEUROPROTECTION; NEWBORN; NONHUMAN; NUCLEOTIDE SEQUENCE; OUTCOME ASSESSMENT; PROTEIN EXPRESSION; RAT; REVERSE TRANSCRIPTION POLYMERASE CHAIN REACTION; SCIATIC NERVE; SPINAL CORD MOTONEURON; SPINAL CORD TRANSSECTION; SYSTEMIC THERAPY; TREATMENT DURATION; WESTERN BLOTTING; WISTAR RAT;

RATTUS; RATTUS NORVEGICUS;

EID: 59849129417     PISSN: 0100879X     EISSN: 1414431X     Source Type: Journal    
DOI: 10.1590/S0100-879X2008005000052     Document Type: Article

References (16)

1. Lawson SJ, Lowrie MB. The role of apoptosis and excitotoxicity in the death of spinal motoneurons and interneurons after neonatal nerve injury. Neuroscience 1998; 87: 337-348.
2. Ikeda K, Iwasaki Y, Shiojima T, Kinoshita M. Neuroprotective effect of various cytokines on developing spinal motoneurons following axotomy. J Neurol Sci 1996; 135: 109-113.
3. Sendtner M, Kreutzberg GW, Thoenen H. Ciliary neurotrophic factor prevents the degeneration of motor neurons after axotomy. Nature 1990; 345: 440-441.
4. Oliveira AL, Risling M, Negro A, Langone F, Cullheim S. Apoptosis of spinal interneurons induced by sciatic nerve axotomy in the neonatal rat is counteracted by nerve growth factor and ciliary neurotrophic factor. J Comp Neurol 2002; 447: 381-393.
5. Maier K, Rau CR, Storch MK, Sattler MB, Demmer I, Weissert R, et al. Ciliary neurotrophic factor protects retinal ganglion cells from secondary cell death during acute autoimmune optic neuritis in rats. Brain Pathol 2004; 14: 378-387.
6. Pan W, Kastin AJ, Maness LM, Brennan JM. Saturable entry of ciliary neurotrophic factor into brain. Neurosci Lett 1999; 263: 69-71.
7. Adams JM, Cory S. The Bcl-2 protein family: arbiters of cell survival. Science 1998; 281: 1322-1326.
8. Kinugasa T, Ozaki S, Hamanaka S, Kudo N. The effects of sciatic nerve axotomy on spinal motoneurons in neonatal Bax-deficient mice. Neurosci Res 2002; 44: 439-446.
9. Rogerio F, de Souza QL, Teixeira SA, Oliveira AL, de Nucci G, Langone F. Neuroprotective action of melatonin on neonatal rat motoneurons after sciatic nerve transection. Brain Res 2002; 926: 33-41.
10. Dlugosz PJ, Billen LP, Annis MG, Zhu W, Zhang Z, Lin J, et al. Bcl-2 changes conformation to inhibit Bax oligomerization. EMBO J 2006; 25: 2287-2296.
11. Dubois-Dauphin M, Frankowski H, Tsujimoto Y, Huarte J, Martinou JC. Neonatal motoneurons overexpressing the bcl-2 protooncogene in transgenic mice are protected from axotomy-induced cell death. Proc Natl Acad Sci U S A 1994; 91: 3309-3313.
12. de Bilbao F, Dubois-Dauphin M. Time course of axotomy-induced apoptotic cell death in facial motoneurons of neonatal wild type and bcl-2 transgenic mice. Neuroscience 1996; 71: 1111-1119.
13. Ojala PM, Yamamoto K, Castanos-Velez E, Biberfeld P, Korsmeyer SJ, Makela TP. The apoptotic v-cyclin-CDK6 complex phosphorylates and inactivates Bcl-2. Nat Cell Biol 2000; 2: 819-825.
14. Kirsch M, Schneider T, Lee MY, Hofmann HD. Lesion-induced changes in the expression of ciliary neurotrophic factor and its receptor in rat optic nerve. Glia 1998; 23: 239-248.
15. Albrecht PJ, Dahl JP, Stoltzfus OK, Levenson R, Levison SW. Ciliary neurotrophic factor activates spinal cord astrocytes, stimulating their production and release of fibroblast growth factor-2, to increase motor neuron survival. Exp Neurol 2002; 173: 46-62.
16. Rios-Munoz W, Soto I, Duprey-Diaz MV, Blagburn J, Blanco RE. Fibroblast growth factor 2 applied to the optic nerve after axotomy increases Bcl-2 and decreases Bax in ganglion cells by activating the extracellular signal-regulated kinase signaling pathway. J Neurochem 2005; 93: 1422-1433.