Ultramicrotomy in the ESEM, a versatile method for materials and life sciences

Journal of Microscopy

Volumn 233, Issue 1, 2009, Pages 140-148

  Zankel, A ^a   Kraus, B ^b   Poelt, P ^a   Schaffer, M ^a   Ingolic, E ^a  

^a GRAZ UNIVERSITY OF TECHNOLOGY   (Austria)

^b GATAN INC   (United States)

Author keywords

ESEM; In situ microtomy; SBFSEM; Three dimensional; Tomography; Ultramicrotomy

Indexed keywords

AUTOMATION; TOMOGRAPHY;

ENVIRONMENTAL SCANNING ELECTRON MICROSCOPES; ESEM; IMAGING TOOLS; IN SITU MICROTOMY; LIFE SCIENCES RESEARCH; LIFE-SCIENCES; MATERIAL SCIENCE; SBFSEM; ULTRA-MICROTOMY; VERSATILE METHODS;

SCANNING ELECTRON MICROSCOPY;

CELLULOSE; RESIN;

ARTICLE; COMPOSITE MATERIAL; DATA ANALYSIS; DEVICE; ENVIRONMENTAL SCANNING ELECTRON MICROSCOPY; IMAGE ANALYSIS; IMAGE QUALITY; IMAGING SYSTEM; MATERIALS TESTING; MICROTOME; OPTICAL RESOLUTION; PRIORITY JOURNAL; SCANNING ELECTRON MICROSCOPY; SERIAL BLOCK FACE SCANNING ELECTRON MICROSCOPY; THREE DIMENSIONAL IMAGING; ULTRAMICROTOMY;

EID: 58449096443     PISSN: 00222720     EISSN: 13652818     Source Type: Journal    
DOI: 10.1111/j.1365-2818.2008.03104.x     Document Type: Article

References (30)

1. Blaschke, E. (2005) Mechanische Eigenschaften von Holz- Kunststoffverbunden. Tagungsband zum Seminar Holz und Kunststoff im Verbund (ed. by the Association of the Austrian Wood Industries pp. 44 53. Kapfenberg, Austria.
2. Denk, W. & Horstmann, H. (2004) Serial block-face scanning electron microscopy to reconstruct three-dimensional tissue nanostructure. PloS Biol. 2 (11 1900 1909.
3. Diaspro, A. & Robello, M. (2000) Two-photon excitation of fluorescence for three-dimensional optical imaging of biological structures. J. Photochem. Photobiol. B, Biol. 55, 1 8.
4. Dickie, R., Bachoo, R.M., Rupnick, M.A., et al 2006) Three-dimensional visualization of microvessel architecture of whole-mount tissue by confocal microscopy. Microvasc. Res. 72, 20 26.
5. Donoser, M., Wiltsche, M. & Bischof, H. (2005) A new automated microtomy concept for 3D paper structure analysis. Proceedings of IAPR Conference on Machine Vision Applications - MVA 2005, pp. 76-79. Tsukuba Science City, Japan.
6. Dunn, D.N. & Hull, R. (1999) Reconstruction of three-dimensional chemistry and geometry using focused ion beam microscopy. Appl. Phys. Lett. 75, 3414 3416.
7. Efimov, A.E., Tonevitsky, A.G., Dittrich, M. & Matsko, N.B. (2007) Atomic force microscope (AFM) combined with the ultramicrotome: a novel device for the serial section tomography and AFM/TEM complementary structural analysis of biological and polymer samples. J. Microsc. 226, 207 217.
8. FEI Company (2003) The Quanta FEG 200, 400, 600. User's Operation Manual Ch. 9, 10 12.
9. Goldstein, J.I., Newbury, D.E., Echlin, P., et al 2003) Scanning Electron Microscopy and X-Ray Microanalysis, 3rd edn. Kluwer Academic /Plenum Publishers, New York.
10. Harris, K.M., Perry, E., Bourne, J., Feinberg, M., Ostroff, L. & Hurlburt, J. (2006) Uniform serial sectioning for transmission electron microscopy. J. Neurosci. 26, 12101 12103.
11. 3 ceramics using FIB nanotomography. J. Microsc. 216, 84 95.
12. Hoppe, W. (1981) Three-dimensional electron microscopy. Ann. Rev. Biophys. Bioeng. 10, 563 592.
13. Inkson, B.J., Mulvihill, M. & Möbus, G. (2001) 3D determination of grain shape in a FeAl-based nanocomposite by 3D FIB tomography. Scripta Materialia 45, 753 758.
14. Kitching, S. & Donald, A.M. (1998) Beam damage of polypropylene in the environmental scanning electron microscope: an FTIR study. J. Microsc. 190, 357 365.
15. Kotula, P.G., Keenan, M.R. & Michael, J.R. (2006) Tomographic spectral imaging with multivariate statistical analysis: comprehensive 3D microanalysis. Microsc. Microanal. 12, 36 48.
16. Leighton, B., St. (1981) SEM images of block faces, cut by a miniature microtome within the SEM-A technical note. Scan. Electron Micros. 2, 73 76.
17. Macke, J.H., Maack, N., Gupta, R., Denk, W., Schoelkopf, B. & Borst, A. (2008) Contour-propagation algorithms for semi-automated reconstruction of neural processes. J. Neurosci. Methods 167, 349 357.
18. Michler, G.H. (1992) Kunststoff-Mikromechanik. Carl Hanser Verlag, Munich.
19. Michler, G.H., Lebek, W., Godehardt, R., Galetzka, W., Gnägi, H. & Vastenhout, B. (2004) Ultramikrotomie in der Materialforschung. Carl Hanser Verlag, Munich.
20. Möbus, G. & Inkson, B.J. (2007) Nanoscale tomography in materials science. Mater. Today 10, 18 25.
21. Park, K.H., Kishimoto, H. & Kohyama, A. (2004) 3D analysis of cracking behaviour under indentation in ion-irradiated β-SiC. J. Electron Microsc. 53, 511 513.
22. Poelt, P., Zankel, A., Gahleitner, M., Grein, C. & Ingolic, E. (2006) The fracture of polymers - tensile tests in the environmental scanning electron microscope. Book of Abstracts, 13th International Conference on Deformation, Yield and Fracture of Polymers (DYFP) 2006 (ed. by H.E.H. Meijer pp. 331 334. Kerkrade, The Netherlands.
23. Rakic, P., Stensas, L.J., Sayre, E.P. & Sidman, R.L. (1974) Computer-aided three-dimensional reconstruction and quantitative analysis of cells from serial electron microscopic montages of foetal monkey brain. Nature 250, 31 34.
24. Royall, C.P., Thiel, B.L. & Donald, A.M. (2001) Radiation damage of water in environmental scanning electron microscopy. J. Microsc. 204, 185 195.
25. Sawyer, L.C. & Grubb, D.T. (1996) Polymer Microscopy, 2nd edn. Chapman and Hall, London.
26. Schaffer, M., Wagner, J., Schaffer, B., Schmied, M. & Mulders, H. (2007) Automated three-dimensional x-ray analysis using a dual-beam FIB. Ultramicroscopy 107, 587 597.
27. Stokes, D.J. (2006) Investigating biological ultrastructure using environmental scanning electron microscopy (ESEM). Science, Technology and Education of Microscopy: An Overview, pp. 564 570. Available at http://www.formatex.org/microscopy1/65.pdf.
28. Xu, W., Ferry, M., Mateescu, N., Cairney, J.M. & Humphreys, F.J. (2007) Techniques for generating 3-D EBSD microstructures by FIB tomography. Mater. Character. 58, 961 967.
29. Zankel, A., Poelt, P., Gahleitner, M., Ingolic, E. & Grein, C. (2007) Tensile tests of polymers at low temperatures in the environmental scanning electron microscope: an improved cooling platform. Scanning 29, 261 269.
30. Zankel, A., Chernev, B., Brandl, C.H., et al 2008) Assessment of beam damage in polymers caused by in situ ESEM analysis using IR spectroscopy. Macromol. Symp. 265, 156 165.