Iron feeding induces ferroportin 1 and hephaestin migration and interaction in rat duodenal epithelium

American Journal of Physiology - Gastrointestinal and Liver Physiology

Volumn 296, Issue 1, 2009, Pages

  Yeh, Kwo Yih ^a   Yeh, Mary ^a   Mims, Laura ^a   Glass, Jonathan ^a  

^a LOUISIANA STATE UNIVERSITY HEALTH SCIENCES CENTER   (United States)

Author keywords

Blue native SDS PAGE; Coimmunoprecipitation; DsRed FPN1; Emgfp Heph; FPN1 and heph migration

Indexed keywords

BETA ACTIN; DIMER; FERROPORTIN 1; GREEN FLUORESCENT PROTEIN; HEPHAESTIN; IRON; MONOMER;

ANIMAL TISSUE; APICAL MEMBRANE; ARTICLE; BASOLATERAL MEMBRANE; BRUSH BORDER; CARBOXY TERMINAL SEQUENCE; CELL STRAIN HEK293; CYTOPLASM; DUODENUM; FEEDING; HUMAN; HUMAN CELL; IMMUNOPRECIPITATION; INTESTINE EPITHELIUM CELL; IRON ABSORPTION; IRON INTAKE; IRON TRANSPORT; NONHUMAN; POLYACRYLAMIDE GEL ELECTROPHORESIS; PRIORITY JOURNAL; PROTEIN LOCALIZATION; PROTEIN PROTEIN INTERACTION; RAT; ULTRACENTRIFUGATION;

ANIMALS; ANTIBODY SPECIFICITY; CATION TRANSPORT PROTEINS; CELL LINE; CENTRIFUGATION, DENSITY GRADIENT; CYTOPLASMIC VESICLES; DUODENUM; ENTEROCYTES; FERROUS COMPOUNDS; HUMANS; IMMUNE SERA; IMMUNOPRECIPITATION; INTESTINAL ABSORPTION; IRON, DIETARY; MEMBRANE PROTEINS; MICROSCOPY, FLUORESCENCE; PROTEIN MULTIMERIZATION; PROTEIN TRANSPORT; RATS; RATS, SPRAGUE-DAWLEY; RECOMBINANT FUSION PROTEINS; TIME FACTORS; TRANSFECTION;

EID: 58249101179     PISSN: 01931857     EISSN: 15221547     Source Type: Journal    
DOI: 10.1152/ajpgi.90298.2008     Document Type: Article

References (42)

1. Abboud S, Haile DJ. A novel mammalian iron-regulated protein involved in intracellular iron metabolism. J Biol Chem 275: 19906-19912, 2000.
2. Arosio P, Levi S. Ferritin, iron homeostasis, and oxidative damage. Free Radic Biol Med 33: 457-463, 2002.
3. Babusiak M, Man P, Petrak J, Vyoral D. Native proteomic analysis of protein complexes in murine intestinal brush border membranes. Proteomics 7: 121-129, 2007.
4. Bjerknes M, Cheng H. Methods for the isolation of intact epithelium from the mouse intestine. Anat Rec 199: 565-574, 1981.
5. Chen H, Huang G, Su T, Gao H, Attieh ZK, McKie AT, Anderson GJ, Vulpe CD. Decreased hephaestin activity in the intestine of copper-deficient mice causes systemic iron deficiency. J Nutr 136: 1236-1241, 2006.
6. Corsi B, Perrone F, Bourgeois M, Beaumont C, Panzeri MC, Cozzi A, Sangregorio R, Santambrogio P, Albertini A, Arosio P, Levi S. Transient overexpression of human H- and L-ferritin chains in COS cells. Biochem J 330: 315-320, 1998.
7. De Domenico I, Ward DM, Musci G, Kaplan J. Evidence for the multimeric structure of ferroportin. Blood 109: 2205-2209, 2007.
8. Devalia V, Carter K, Walker AP, Perkins SJ, Worwood M, May A, Dooley JS. Autosomal dominant reticuloendothelial iron overload associated with a 3-base pair deletion in the ferroportin 1 gene (SLC11A3). Blood 100: 695-697, 2002.
9. Donovan A, Brownlie A, Zhou Y, Shepard J, Pratt SJ, Moynihan J, Paw BH, Drejer A, Barut B, Zapata A, Law TC, Brugnara C, Lux SE, Pinkus GS, Pinkus JL, Kingsley PD, Palis J, Fleming MD, Andrews NC, Zon LI. Positional cloning of zebrafish ferroportin1 identifies a conserved vertebrate iron exporter. Nature 403: 776-781, 2000.
10. Donovan A, Lima CA, Pinkus JL, Pinkus GS, Zon LI, Robine S, Andrews NC. The iron exporter ferroportin/Slc40a1 is essential for iron homeostasis. Cell Metab 1: 191-200, 2005.
11. Frazer DM, Vulpe CD, McKie AT, Wilkins SJ, Trinder D, Cleghorn GJ, Anderson GJ. Cloning and gastrointestinal expression of rat hephaestin: relationship to other iron transport proteins. Am J Physiol Gastrointest Liver Physiol 281: G931-G939, 2001.
12. Frazer DM, Wilkins SJ, Becker EM, Murphy TL, Vulpe CD, McKie AT, Anderson GJ. A rapid decrease in the expression of DMT1 and Dcytb but not Ireg1 or hephaestin explains the mucosal block phenomenon of iron absorption. Gut 52: 340-346, 2003.
13. Ganz T, Nemeth E. Iron imports. IV. Hepcidin and regulation of body iron metabolism. Am J Physiol Gastrointest Liver Physiol 290: G199 - G203, 2006.
14. Gleeson F, Ryan E, Barrett S, Russell J, Kelleher B, Crowe J. Duodenal Dcytb and hephaestin mRNA expression are not significantly modulated by variations in body iron homeostasis. Blood Cells Mol Dis 35: 303-308, 2005.
15. Goncalves AS, Muzeau F, Blaybel R, Hetet G, Driss F, Delaby C, Canonne-Hergaux F, Beaumont C. Wild-type and mutant ferroportins do not form oligomers in transfected cells. Biochem J 396: 265-275, 2006.
16. Goossen B, Caughman SW, Harford JB, Klausner RD, Hentze MW. Translational repression by a complex between the iron-responsive element of ferritin mRNA and its specific cytoplasmic binding protein is position-dependent in vivo. EMBO J 9: 4127-4133, 1990.
17. Gunshin H, Mackenzie B, Berger UV, Gunshin Y, Romero MF, Boron WF, Nussberger S, Gollan JL, Hediger MA. Cloning and characterization of a mammalian proton-coupled metal-ion transporter. Nature 388:482-488, 1997.
18. Hahn PF, Bale WF, Ross JF, Balfour WM, Whipple GH. Radioactive iron absorption by gastro-intestinal tract: influence of anemia, anoxia, and antecedent feeding distribution in growing dogs. J Exp Med 78: 169-188,1943.
19. Han O, Kim EY. Colocalization of ferroportin-1 with hephaestin on the basolateral membrane of human intestinal absorptive cells. J Cell Biochem 101: 1000-1010, 2007.
20. Hentze MW, Muckenthaler MU, Andrews NC. Balancing acts:molecular control of mammalian iron metabolism. Cell 117: 285-297, 2004.
21. Kuo YM, Su T, Chen H, Attieh Z, Syed BA, McKie AT, Anderson GJ, Gitschier J, Vulpe CD. Mislocalisation of hephaestin, a multicopper ferroxidase involved in basolateral intestinal iron transport, in the sex linked anaemia mouse. Gut 53: 201-206, 2004.
22. Linder MC, Dunn V, Isaacs E, Jones D, Lim S, Van Volkom M, Munro HN. Ferritin and intestinal iron absorption: pancreatic enzymes and free iron. Am J Physiol 228: 196-204, 1975.
23. Liu XB, Yang F, Haile DJ. Functional consequences of ferroportin 1 mutations. Blood Cells Mol Dis 35: 33-46, 2005.
24. Ma Y, Specian RD, Yeh KY, Yeh M, Rodriguez-Paris J, Glass J. The transcytosis of divalent metal transporter 1 and apo-transferrin during iron uptake in intestinal epithelium. Am J Physiol Gastrointest Liver Physiol 283: G965-G974, 2002.
25. Ma Y, Yeh M, Yeh KY, Glass J. Iron imports. V. Transport of iron through the intestinal epithelium. Am J Physiol Gastrointest Liver Physiol 290: G417-G422, 2006.
26. Mascotti DP, Rup D, Thach RE. Regulation of iron metabolism: translational effects mediated by iron, heme, and cytokines. Annu Rev Nutr 15: 239-261, 1995.
27. McKie AT, Barrow D, Latunde-Dada GO, Rolfs A, Sager G, Mudaly E, Mudaly M, Richardson C, Barlow D, Bomford A, Peters TJ, Raja KB, Shirali S, Hediger MA, Farzaneh F, Simpson RJ. An iron-regulated ferric reductase associated with the absorption of dietary iron. Science 291: 1755-1759, 2001.
28. McKie AT, Marciani P, Rolfs A, Brennan K, Wehr K, Barrow D, Miret S, Bomford A, Peters TJ, Farzaneh F, Hediger MA, Hentze MW, Simpson RJ. A novel duodenal iron-regulated transporter, IREG1, implicated in the basolateral transfer of iron to the circulation. Mol Cell 5: 299-309, 2000.
29. Mok H, Jelinek J, Pai S, Cattanach BM, Prchal JT, Youssoufian H, Schumacher A. Disruption of ferroportin 1 regulation causes dynamic alterations in iron homeostasis and erythropoiesis in polycythaemia mice. Development 131: 1859-1868, 2004.
30. Nemeth E, Tuttle MS, Powelson J, Vaughn MB, Donovan A, Ward DM, Ganz T, Kaplan J. Hepcidin regulates cellular iron efflux by binding to ferroportin and inducing its internalization. Science 306: 2090-2093, 2004.
31. Nicolas G, Bennoun M, Devaux I, Beaumont C, Grandchamp B, Kahn A, Vaulont S. Lack of hepcidin gene expression and severe tissue iron overload in upstream stimulatory factor 2 (USF2) knockout mice. Proc Natl Acad Sci USA 98: 8780-8785, 2001.
32. Nittis T, Gitlin JD. Role of copper in the proteosome-mediated degradation of the multicopper oxidase hephaestin. J Biol Chem 279: 25696 -25702, 2004.
33. Reeves PG, Demars LC, Johnson WT, Lukaski HC. Dietary copper deficiency reduces iron absorption and duodenal enterocyte hephaestin protein in male and female rats. J Nutr 135: 92-98, 2005.
34. Schagger H, Cramer WA, von Jagow G. Analysis of molecular masses and oligomeric states of protein complexes by blue native electrophoresis and isolation of membrane protein complexes by two-dimensional native electrophoresis. Anal Biochem 217: 220-230, 1994.
35. Stewart WB, Yuile CL, Claiborne HA, Snowman RT, Whipple GH. Radioiron absorption in anemic dogs; fluctuations in the mucosal block and evidence for a gradient of absorption in the gastrointestinal tract. J Exp Med 92: 375-382, 1950.
36. Swamy M, Siegers GM, Minguet S, Wollscheid B, Schamel WW. Blue native polyacrylamide gel electrophoresis (BN-PAGE) for the identification and analysis of multiprotein complexes. Sci STKE 2006: pl4, 2006.
37. Syed BA, Beaumont NJ, Patel A, Naylor CE, Bayele HK, Joannou CL, Rowe PS, Evans RW, Srai SK. Analysis of the human hephaestin gene and protein: comparative modelling of the N-terminus ecto-domain based upon ceruloplasmin. Protein Eng 15: 205-214, 2002.
38. Vulpe CD, Kuo YM, Murphy TL, Cowley L, Askwith C, Libina N, Gitschier J, Anderson GJ. Hephaestin, a ceruloplasmin homologue implicated in intestinal iron transport, is defective in the sla mouse. Nat Genet 21: 195-199, 1999.
39. Yeh KY, Yeh M, Glass J. Dietary iron induces ferroportin 1 clustering and migration into the basolateral membrane of the rat intestinal epithelium (Abstract). Gastroenterology 120: A678, 2001.
40. Yeh KY, Yeh M, Glass J. Hepcidin regulation of ferroportin 1 expression in the liver and intestine of the rat. Am J Physiol Gastrointest Liver Physiol 286: G385-G394, 2004.
41. Yeh KY, Yeh M, Watkins JA, Rodriguez-Paris J, Glass J. Dietary iron induces rapid changes in rat intestinal divalent metal transporter expression. Am J Physiol Gastrointest Liver Physiol 279: G1070-G1079, 2000.
42. + exchange. Annu Rev Physiol 67: 411-443, 2005.