Multiplex RT-PCR expression analysis of developmentally important genes in individual mouse preimplantation embryos and blastomeres

Biology of Reproduction

Volumn 80, Issue 1, 2009, Pages 194-202

  May, Andreas ^a   Kirchner, Roland ^b   Müller, Helena ^b   Hartmann, Petra ^b   Hajj, Nady El ^a   Tresch, Achim ^a   Zechner, Ulrich ^a   Mann, Wolfgang ^b   Haaf, Thomas ^a  

^a JOHANNES GUTENBERG UNIVERSITY   (Germany)

^b Advalytix AG   (Germany)

Author keywords

Blastomere; Developmental biology; DNA methyltransferase; Early development; Embryo; Gene regulation; Genome reprogramming; In vitro fertilization; Methyl CpG binding domain protein; Multiplex RT PCR; Preimplantation development; Single cell analysis

Indexed keywords

DNA METHYLTRANSFERASE 3A; HISTONE H3F3A; MESSENGER RNA; METHYL CPG BINDING PROTEIN; METHYLTRANSFERASE; NUCLEAR PROTEIN; OCTAMER TRANSCRIPTION FACTOR 4; TRANSCRIPTOME; UNCLASSIFIED DRUG;

ANIMAL CELL; ARTICLE; BACTERIAL GENE; BLASTOMERE; CELL NUCLEUS; CHROMATIN STRUCTURE; CONTROLLED STUDY; DEMETHYLATION; DNA METHYLATION; DNA MODIFICATION; EMBRYO; EXCISION REPAIR; FEMALE; FERTILIZATION IN VITRO; GENE ACTIVITY; GENE CONTROL; GENE EXPRESSION; GENETIC ANALYSIS; GENETIC TRANSCRIPTION; GENOME; IMMUNOLOCALIZATION; MALE; MATING; MICROFLUIDIC ANALYSIS; MORULA; MOUSE; NONHUMAN; PLURIPOTENT STEM CELL; PREIMPLANTATION EMBRYO; PRIORITY JOURNAL; PROTEIN EXPRESSION; PROTEIN VARIANT; QUALITATIVE ANALYSIS; REVERSE TRANSCRIPTION POLYMERASE CHAIN REACTION; SUPEROVULATION;

ANIMALS; BLASTOCYST; BLASTOMERES; DNA; EMBRYONIC DEVELOPMENT; FEMALE; GENE EXPRESSION PROFILING; GENE EXPRESSION REGULATION, DEVELOPMENTAL; MICE; MICE, INBRED C57BL; MICROFLUIDIC ANALYTICAL TECHNIQUES; PREGNANCY; REVERSE TRANSCRIPTASE POLYMERASE CHAIN REACTION; TRANSCRIPTION, GENETIC;

EID: 58149485337     PISSN: 00063363     EISSN: 15297268     Source Type: Journal    
DOI: 10.1095/biolreprod.107.064691     Document Type: Article

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