Using affinity chromatography to engineer and characterize pH-dependent protein switches

Protein Science

Volumn 18, Issue 1, 2009, Pages 217-228

  Sagermann, Martin ^a   Chapleau, Richard R ^a   DeLorimier, Elaine ^a   Lei, Margarida ^a  

^a UNIVERSITY OF CALIFORNIA   (United States)

Author keywords

Allostery; Conformational switches; Dynamics; pH sensor; Protein design

Indexed keywords

ASPARTIC ACID; CYSTEINE; GLUTATHIONE; GLUTATHIONE TRANSFERASE; HISTIDINE;

AFFINITY CHROMATOGRAPHY; ARTICLE; BINDING AFFINITY; CONTROLLED STUDY; CRYSTAL STRUCTURE; ENZYME INHIBITION; GENE MUTATION; NONHUMAN; PH MEASUREMENT; PRIORITY JOURNAL; PROTEIN ANALYSIS; PROTEIN ENGINEERING; PROTEIN FUNCTION; PROTEIN INTERACTION; PROTEIN STRUCTURE; THERMOSTABILITY;

ALLOSTERIC REGULATION; AMINO ACID SEQUENCE; AMINO ACID SUBSTITUTION; ANIMALS; CHROMATOGRAPHY, AFFINITY; CIRCULAR DICHROISM; CRYSTALLOGRAPHY, X-RAY; ESCHERICHIA COLI; GLUTATHIONE; GLUTATHIONE TRANSFERASE; HYDROGEN-ION CONCENTRATION; MODELS, MOLECULAR; MUTATION; PROTEIN BINDING; PROTEIN ENGINEERING; PROTEIN STRUCTURE, TERTIARY; SCHISTOSOMA JAPONICUM; WATER;

EID: 58149462498     PISSN: 09618368     EISSN: 1469896X     Source Type: Journal    
DOI: 10.1002/pro.23     Document Type: Article

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