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The two-state model merely provides a convenient framework for analyzing data. Because single-domain proteins are relatively small finite-sized systems, fluctuations play a major role in thermodynamics and kinetics. Consequences of the finite size include, but are not limited to, ruggedness of the energy landscape even in the folded state, variations in the collapse (or melting) temperatures of different residues, a broad transition-state ensemble, and diversity of folding pathways especially during the early stages of folding. These effects are not considered here
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