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Volumn 303, Issue 5664, 2004, Pages 1674-1678

Force-Clamp Spectroscopy Monitors the Folding Trajectory of a Single Protein

Author keywords

[No Author keywords available]

Indexed keywords

ATOMIC FORCE MICROSCOPY; CONFORMATIONS; QUENCHING; REACTION KINETICS; SPECTROSCOPY;

EID: 1542513548     PISSN: 00368075     EISSN: None     Source Type: Journal    
DOI: 10.1126/science.1092497     Document Type: Article
Times cited : (464)

References (35)
  • 1
    • 0037456298 scopus 로고    scopus 로고
    • U. Mayor et al., Nature 421, 863 (2003).
    • (2003) Nature , vol.421 , pp. 863
    • Mayor, U.1
  • 13
    • 1542563364 scopus 로고    scopus 로고
    • note
    • Materials and methods are available as supporting material on Science Online.
  • 18
    • 1542458956 scopus 로고    scopus 로고
    • note
    • The use of a greatly improved piezoelectric actuator together with soft cantilevers makes it possible to control the force and the length of a single protein with pico-Newton and nanometer resolution. However, it is still somewhat difficult to set the exact value of a low-force setting because our current force-clamp apparatus can be affected by small direct current offsets that affect our zero-force set point. These offsets do not affect our results at high stretching forces but can cause an unknown error when quenching the stretched protein to a low force. One way to independently measure the actual quenched force is to measure the magnitude of the elastic recoil that occurs immediately after relaxing the unfolded polymer chain to the lower force (e.g., steplike relaxation that coincides with downward arrows in Fig. 3). Given that in each case we know the contour length of the unfolded polypeptide, we used the wormlike chain model of polymer elasticity to find the magnitude of the force quench for each elastic recoil event.
  • 35
    • 1542563365 scopus 로고    scopus 로고
    • note
    • We thank L. Li and K. Walther for helpful discussions and H. Huang for technical assistance. This work is funded by grants from NIH (J.M.F.).


* 이 정보는 Elsevier사의 SCOPUS DB에서 KISTI가 분석하여 추출한 것입니다.