Monitoring "de novo" APP synthesis by taking advantage of the reversible effect of cycloheximide

American Journal of Alzheimer's Disease and other Dementias

Volumn 23, Issue 6, 2009, Pages 602-608

  Rebelo, Sandra ^a   Vieira, Sandra Isabel ^a   Da Cruz E Silva, Edgar F ^a   Da Cruz E Silva, Odete A B ^a  

^a UNIVERSITY OF AVEIRO   (Portugal)

Author keywords

"de novo" pro tein synthesis; Anterograde transport; APP GFP; Monitoring; Phosphorylation

Indexed keywords

AMYLOID PRECURSOR PROTEIN; CYCLOHEXIMIDE; GREEN FLUORESCENT PROTEIN;

ANIMAL CELL; ARTICLE; CONTROLLED STUDY; ENDOCYTOSIS; NONHUMAN; PROTEIN ANALYSIS; PROTEIN LOCALIZATION; PROTEIN METABOLISM; PROTEIN MOTIF; PROTEIN PHOSPHORYLATION; PROTEIN SYNTHESIS; TRANS GOLGI NETWORK;

EID: 58149191628     PISSN: 15333175     EISSN: None     Source Type: Journal    
DOI: 10.1177/1533317508323572     Document Type: Article

References (19)

1. Weihofen A., Martoglio B. Intramembrane-cleaving proteases: controlled liberation of proteins and bioactive peptides. Trends Cell Biol. 2003 ; 13: 71-78.
2. Xu H., Greengard P., Gandy S. Regulated formation of Golgi secretory vesicles containing Alzheimer beta-amyloid precursor protein. J Biol Chem. 1995 ; 270: 23243-23245.
3. Buxbaum JD, Koo EH, Greengard P. Protein phosphorylation inhibits production of Alzheimer amyloid beta/A4 peptide. Proc Natl Acad Sci U S A. 1993 ; 90: 9195-9198.
4. Ando K., Iijima KI, Elliott JI, Kirino Y., Suzuki T. Phosphorylation- dependent regulation of the interaction of amyloid precursor protein with Fe65 affects the production of beta-amyloid. J Biol Chem. 2001 ; 276: 40353-40361.
5. Chen WJ, Goldstein JL, Brown MS NPXY, a sequence often found in cytoplasmic tails, is required for coated pit-mediated internalization of the low density lipoprotein receptor. J Biol Chem. 1990 ; 265: 3116-3123.
6. Koo EH, Squazzo SL Evidence that production and release of amyloid beta-protein involves the endocytic pathway. J Biol Chem. 1994 ; 269: 17386-17389.
7. Lai A., Sisodia SS, Trowbridge IS Characterization of sorting signals in the beta-amyloid precursor protein cytoplasmic domain. J Biol Chem. 1995 ; 270: 3565-3573.
8. Zambrano N., Bruni P., Minopoli G., et al. The beta-amyloid precursor protein APP is tyrosine-phosphorylated in cells expressing a constitutively active form of the Abl protoncogene. J Biol Chem. 2001 ; 276: 19787-19792.
9. Tarr PE, Roncarati R., Pelicci G., Pelicci PG, D'Adamio L. Tyrosine phosphorylation of the beta-amyloid precursor protein cytoplasmic tail promotes interaction with Shc. J Biol Chem. 2002 ; 277: 16798-16804.
10. Lee MS, Kao SC, Lemere CA, et al. APP processing is regulated by cytoplasmic phosphorylation. J Cell Biol. 2003 ; 163: 83-95.
11. Rebelo S., Vieira SI, Esselmann H., Wiltfang J., da Cruz e Silva EF, da Cruz e Silva OA Tyrosine 687 phosorylated Alzheimer's amyloid precursor protein is retained intracellularly and exhibits a decreased turnover rate. Neurodegener Dis. 2007 ; 4: 78-87.
12. Rebelo S., Vieira SI, Esselmann H., Wiltfang J., da Cruz e Silva EF, da Cruz e Silva OAB. Try687 dependent APP endocytosis and Abeta production. J Mol Neurosci. 2007 ; 32: 1-8.
13. da Cruz e Silva O., Vieira SI, Rebelo S., da Cruz e Silva EF A model system to study intracellular trafficking and processing of the Alzheimer's amyloid precursor protein. Neurodegener Dis. 2004 ; 1: 196-204.
14. da Cruz e Silva OA, Iverfeldt K., Oltersdorf T., et al. Regulated cleavage of Alzheimer beta-amyloid precursor protein in the absence of the cytoplasmic tail. Neuroscience. 1993 ; 57: 873-877.
15. Rebelo S., Henriques AG, da Cruz e Silva EF, da Cruz e Silva OA Effect of cell density on intracellular levels of the Alzheimer's amyloid precursor protein. J Neurosci Res. 2004 ; 76: 406-414.
16. Shin RW, Saido TC, Maeda M., Kitamoto T. Novel alphasecretase cleavage of Alzheimer's amyloid beta precursor protein in the endoplasmic reticulum of COS7 cells. Neurosci Lett. 2005 ; 376: 14-19.
17. Selkoe DJ, Yamazaki T., Citron M., et al. The role of APP processing and trafficking pathways in the formation of amyloid beta-protein. Ann N Y Acad Sci. 1996 ; 777: 57-64.
18. Greenfield JP, Tsai J., Gouras GK, et al. Endoplasmic reticulum and trans-Golgi network generate distinct populations of Alzheimer beta-amyloid peptides. Proc Natl Acad Sci U S A. 1999 ; 96: 742-747.
19. Perez RG, Soriano S., Hayes JD, et al. Mutagenesis identifies new signals for beta-amyloid precursor protein endocytosis, turnover, and the generation of secreted fragments, including Abeta42. J Biol Chem. 1999 ; 274: 18851-18856.