Use of Kaede fusions to visualize recycling of G protein-coupled receptors

Traffic

Volumn 10, Issue 1, 2009, Pages 2-15

  Schmidt, Antje ^a   Wiesner, Burkhard ^a   Weißhart, Klaus ^b   Schulz, Katharina ^a   Furkert, Jens ^a   Lamprecht, Björn ^c   Rosenthal, Walter ^a,d   Schülein, Ralf ^a  

^a LEIBNIZ INSTITUT FÜR MOLEKULARE PHARMAKOLOGIE   (Germany)

^b CARL ZEISS SMT AG   (Germany)

^c MAX DELBRÜCK CENTER FOR MOLECULAR MEDICINE   (Germany)

^d CHARITÉ UNIVERSITÄTSMEDIZIN BERLIN   (Germany)

Author keywords

Confocal laser scanning microscopy; Corticotropin releasing factor receptor type 1; Endosomes; G protein coupled receptors; Internalization; Kaede; Lysosomes; Recycling

Indexed keywords

CORTICOTROPIN RELEASING FACTOR; CORTICOTROPIN RELEASING FACTOR RECEPTOR TYPE 1; G PROTEIN COUPLED RECEPTOR; GREEN FLUORESCENT PROTEIN; HYBRID PROTEIN; KAEDE PROTEIN; MEMBRANE PROTEIN; UNCLASSIFIED DRUG;

ARTICLE; CELL FUSION; CELL MEMBRANE TRANSPORT; CONTROLLED STUDY; ENDOSOME; FLUORESCENCE; HUMAN; HUMAN CELL; INTERNALIZATION; METHODOLOGY; NONHUMAN; OLIGOMERIZATION; PRIORITY JOURNAL; PROTEIN SECRETION; SIGNAL TRANSDUCTION; SPECTROSCOPY; ULTRAVIOLET IRRADIATION;

ANIMALS; CELL LINE; HUMANS; LIGANDS; LUMINESCENT PROTEINS; MICROSCOPY, FLUORESCENCE; PHOTOCHEMISTRY; RATS; RECEPTORS, G-PROTEIN-COUPLED; TIME FACTORS;

EID: 58149191234     PISSN: 13989219     EISSN: 16000854     Source Type: Journal    
DOI: 10.1111/j.1600-0854.2008.00843.x     Document Type: Article

References (39)

1. Hutter H. Fluorescent reporter methods. Methods Mol Biol 2006; 351: 155-173.
2. Reits EA, Neefjes JJ. From fixed to FRAP: Measuring protein mobility and activity in living cells. Nat Cell Biol 2001; 3: E145-E147.
3. Dundr M, Hebert MD, Karpova TS, Stanek D, Xu H, Shpargel KB, Meier UT, Neugebauer KM, Matera AG, Misteli T. In vivo kinetics of Cajal body components. J Cell Biol 2004; 164: 831-842.
4. Jares-Erijman EA, Jovin TM. Imaging molecular interactions in living cells by FRET microscopy. Curr Opin Chem Biol 2006; 10: 409-416.
5. Brunet S, Zimmermann T, Reynaud EG, Vernos I, Kartensi E, Pepperkok R. Detection and quantification of protein-microtubules interactions using green fluorescent protein photoconversion. Traffic 2006; 7: 1283-1289.
6. Ando R, Hama H, Yamamoto-Hino M, Mizuno H, Miyawaki A. An optical marker based on the UV-induced green-to-red photoconversion of a fluorescent protein. Proc Natl Acad Sci U S A 2002; 99: 12651-12656.
7. Mizuno H, Mal TK, Tong KI, Ando R, Furuta T, Ikura M, Miyawaki A. Photo-induced peptide cleavage in the green-to-red conversion of a fluorescent protein. Mol Cell 2003; 12: 1051-1058.
8. Hauger RL, Grigoriadis DE, Dallman MF, Plotsky PM, Vale WW, Dautzenberg FM. International Union of Pharmacology. XXXVI. Current status of the nomenclature for receptors for corticotropin-releasing factor and their ligands. Pharmacol Rev 2003; 55: 21-26.
9. Dautzenberg FM, Hauger RL. The CRF peptide family and their receptors: yet more partners discovered. Trends Pharmacol Sci 2002; 23: 71-77.
10. Bale TL, Vale WW. CRF and CRF receptors: Role in stress responsivity and other behaviors. Annu Rev Pharmacol Toxicol 2004; 44: 525-557.
11. Perry SJ, Junger S, Kohout TA, Hoare SRJ, Struthers RS, Grigoriadis DE, Maki RA. Distinct conformations of the corticotropin releasing factor type 1 receptor adopted following agonist and antagonist binding are differentially regulated. J Biol Chem 2005; 280: 11560-11568.
12. Rasmussen TN, Ivana Novak I, Nielsen SM. Internalization of the human CRF receptor 1 is independent of classical phosphorylation sites and of beta-arrestin 1 recruitment. Eur J Biochem 2004; 271: 4366-4374.
13. Alken M, Rutz C, Köchl R, Donalies U, Oueslati M, Furkert J, Wietfeld D, Hermosilla R, Scholz A, Beyermann M, Rosenthal W, Schülein R. The signal peptide of the rat corticotropin-releasing factor receptor 1 promotes receptor expression but is not essential for establishing a functional receptor. Biochem J 2005; 390: 455-464.
14. Innamorati G, Le Gouill C, Balamotis M, Birnbaumer M. The long and the short cycle. Alternative intracellular routes for trafficking of G-protein-coupled receptors. J Biol Chem 2001; 276: 13096-13103.
15. Robben JH, Knoers NV, Deen PM. Regulation of the vasopressin V2 receptor by vasopressin in polarized renal collecting duct cells. Mol Biol Cell 2004; 15: 5693-5699.
16. Bouley R, Lin HY, Raychowdhury MK, Marshansky V, Brown D, Ausiello DA. Downregulation of the vasopressin type 2 receptor after vasopressin-induced internalization: Involvement of a lysosomal degradation pathway. Am J Physiol Cell Physiol 2005; 288: C1390-C1401.
17. Shenoy SK, Lefkowitz RJ. Multifaceted roles of beta-arrestins in the regulation of seven-membrane-spanning receptor trafficking and signalling. Biochem J 2003; 375: 503-515.
18. Hayashi I, Mizuno H, Tong KI, Furuta T, Tanaka F, Yoshimura M, Miyawaki A, Ikura M. Crystallographic evidence for water-assisted photo-induced peptide cleavage in the stony coral fluorescent protein Kaede. J Mol Biol 2007; 372: 918-926.
19. Ando R, Hama H, Yamamoto-Hino M, Mizuno H, Miyawaki A. An optical marker based on the UV-induced green-to-red photoconversion of a fluorescent protein. Proc Natl Acad Sci U S A 2002; 99: 12651-12656.
20. Heinze KG, Koltermann A, Schwille P. Simultaneous two-photon excitation of distinct labels for dual-color fluorescence crosscorrelation analysis. Proc Natl Acad Sci U S A 2000; 97: 10377-10382.
21. Valdez GR. Development of CRF1 receptor antagonists as antidepressants and anxiolytics: Progress to date. CNS Drugs 2006; 20: 887-896.
22. Bhowmick N, Narayan P, Puett D. The endothelin subtype A receptor undergoes agonist- and antagonist-mediated internalization in the absence of signaling. Endocrinology 1998; 139: 3185-3192.
23. Willins DL, Berry SA, Alsayegh L, Backstrom JR, Sanders-Bush E, Friedman L, Roth BL. Clozapine and other 5-hydroxytryptamine-2A receptor antagonists alter the subcellular distribution of 5-hydroxytryptamine-2A receptors in vitro and in vivo. Neuroscience 1999; 91: 599-606.
24. Raab-Graham KF, Haddick PC, Jan YN, Jan LY. Activity- and mTOR-dependent suppression of Kv1.1 channel mRNA translation in dendrites. Science 2006; 314: 144-148.
25. Schülein R, Hermosilla R, Oksche A, Dehe M, Wiesner B, Krause G, Rosenthal W. A dileucine sequence and an upstream glutamate residue in the intracellular carboxyl terminus of the vasopressin V2 receptor are essential for cell surface transport in COS.M6 cells. Mol Pharmacol 1998; 54: 525-535.
26. Wietfeld D, Heinrich N, Furkert J, Fechner K, Beyermann M, Bienert M, Berger H. Regulation of the coupling to different G proteins of rat corticotropin-releasing factor receptor type 1 in human embryonic kidney 293 cells. J Biol Chem 2004; 279: 38386-38394.
27. Sambrook J, Russel DW. Molecular Cloning: A Laboratory Manual. Cold Spring Harbor Laboratory Press, Cold Spring Harbor, New York, USA; 2001.
28. Rutz C, Renner A, Alken M, Schulz K, Beyermann M, Wiesner B, Rosenthal W, Schülein R. The corticotropin-releasing factor receptor type 2a contains an N-terminal pseudo signal peptide. J Biol Chem 2006; 281: 24910-24921.
29. Grantcharova E, Furkert J, Reusch HP, Krell HW, Papsdorf G, Beyermann M, Schülein R, Rosenthal W, Oksche A. The extracellular N terminus of the endothelin B (ETB) receptor is cleaved by a metalloprotease in an agonist-dependent process. J Biol Chem 2002; 277: 43933-43941.
30. Stein BS, Bensch KG, Sussman HH. Complete inhibition of transferrin recycling by monensin in K562 cells. J Biol Chem 1984; 259: 14762-14772.
31. Kapp-Barnea Y, Ninio-Many L, Hirschberg K, Fukuda M, Jeromin A, Sagi-Eisenberg R. Neuronal calcium sensor-1 and phosphatidylinositol 4-kinase beta stimulate extracellular signal-regulated kinase 1/2 signaling by accelerating recycling through the endocytic recycling compartment. Mol Biol Cell 2006; 17: 4130-4141.
32. Schülein R, Zühlke K, Krause G, Rosenthal W. Functional rescue of the nephrogenic diabetes insipidus-causing vasopressin V2 receptor mutants G185C and R202C by a second site suppressor mutation. J Biol Chem 2001; 276: 8384-8392.
33. Kyhse-Andersen J. Electroblotting of multiple gels: A simple apparatus without buffer tank for rapid transfer of proteins from polyacrylamide to nitrocellulose. J Biochem Biophys Methods 1984; 10: 203-209.
34. Tsao PI, von Zastrow M. Type-specific sorting of G protein-coupled receptors after endocytosis. J Biol Chem 2000; 275: 11130-11140.
35. Rigler R, Mets U, Widengren J, Kask P. Fluorescence correlation spectroscopy with high count rate and low background: Analysis of translational diffusion. Eur Biophys J 1993; 22: 169-175.
36. Elson EL. Fluorescence correlation spectroscopy measures molecular transport in cells. Traffic 2001; 2: 789-796.
37. Haustein E, Schwille P. Ultrasensitive investigations of biological systems by fluorescence correlation spectroscopy. Methods 2003; 29: 153-166.
38. Schwille P, Korlach J, Webb WW. Fluorescence correlation spectroscopy with single-molecule sensitivity on cell and model membranes. Cytometry 1999; 36: 176-182.
39. Schafer SP, Dittrich PS, Petrov EP, Schwille P. Single molecule fluorescence imaging of the photoinduced conversion and bleaching behavior of the fluorescent protein Kaede. Microsc Res Tech 2006; 69: 210-219.