Fluorescence polarization assay for calmodulin binding to plasma membrane Ca2+-ATPase: Dependence on enzyme and Ca2+ concentrations

Analytical Biochemistry

Volumn 385, Issue 1, 2009, Pages 1-6

  Liyanage, Mangala Roshan ^a   Zaidi, Asma ^b   Johnson, Carey K ^a  

^a UNIVERSITY OF KANSAS   (United States)

^b KANSAS CITY UNIVERSITY OF MEDICINE AND BIOSCIENCES   (United States)

Author keywords

Ca2+ ATPase; Calmodulin; Dissociation constant; Fluorescence polarization

Indexed keywords

BINDING ENERGY; BINDING SITES; CALCIUM COMPOUNDS; CELL MEMBRANES; DISSOCIATION; FLUORESCENCE; POLARIZATION;

BINDING AFFINITIES; COOPERATIVE BINDING; DISSOCIATION CONSTANT; FLUORESCENCE POLARIZATION; HIGH THROUGHPUT SCREENING; INDIRECT MEASUREMENTS; KINETICS MEASUREMENTS; SIGNALING PROTEINS;

CALMODULIN;

CALCIUM ION; CALMODULIN BINDING PROTEIN; PLASMA MEMBRANE CALCIUM TRANSPORTING ADENOSINE TRIPHOSPHATASE;

ARTICLE; BINDING AFFINITY; CONCENTRATION (PARAMETERS); DISSOCIATION CONSTANT; ENZYME METABOLISM; FLUORESCENCE POLARIZATION; HIGH THROUGHPUT SCREENING; HUMAN; PRIORITY JOURNAL; PROTEIN BINDING; PROTEIN PROTEIN INTERACTION; TITRIMETRY;

EID: 57749172724     PISSN: 00032697     EISSN: 10960309     Source Type: Journal    
DOI: 10.1016/j.ab.2008.10.022     Document Type: Article

References (54)

1. Perrin F. The polarisation of fluorescence light: Average life of molecules in their excited state. J. Phys. Radium 7 (1926)
2. Dandliker W.B., and De Saussure V.A. Fluorescence polarization in immunochemistry. Immunochemistry 7 (1970) 799-828
3. Royer C.A., Ropp T., and Scarlata S.F. Solution studies of the interactions between the histone core proteins and DNA using fluorescence spectroscopy. Biophys. Chem. 43 (1992) 197-211
4. LeTilly V., and Royer C.A. Fluorescence anisotropy assays implicate protein-protein interactions in regulating trp repressor DNA binding. Biochemistry 32 (1993) 7753-7758
5. Kumke M.U., Li G., McGown L.B., Walker G.T., and Linn C.P. Hybridization of fluorescein-labeled DNA oligomers detected by fluorescence anisotropy with protein binding enhancement. Anal. Chem. 67 (1995) 3945-3951
6. Jameson D.M., and Sawyer W.H. Fluorescence anisotropy applied to biomolecular interactions. Methods Enzymol. 246 (1995) 283-300
7. Kwok P.Y. SNP genotyping with fluorescence polarization detection. Hum. Mutat. 19 (2002) 315-323
8. Checovich W.J., Bolger R.E., and Burke T. Fluorescence polarization: a new tool for cell and molecular biology. Nature 375 (1995) 254-256
9. Heyduk T., Ma Y., Tang H., and Ebright R.H. Fluorescence anisotropy: rapid, quantitative assay for protein-DNA and protein-protein interaction. Methods Enzymol. 274 (1996) 492-503
10. Hill J.J., and Royer C.A. Fluorescence approaches to study of protein-nucleic acid complexation. Methods Enzymol. 278 (1997) 390-416
11. Gibson N.J., Gillard H.L., Whitcombe D., Ferrie R.M., Newton C.R., and Little S. A homogeneous method for genotyping with fluorescence polarization. Clin. Chem. 43 (1997) 1336-1341
12. Jolley M.E., and Nasir M.S. The use of fluorescence polarization assays for the detection of infectious diseases. Comb. Chem. High Throughput Screen. 6 (2003) 235-244
13. Lin M., Sugden E.A., Jolley M.E., and Stilwell K. Modification of the Mycobacterium bovis extracellular protein mpb70 with fluorescein for rapid detection of specific serum antibodies by fluorescence polarization. Clin. Diagn. Lab. Immunol. 3 (1996) 438-443
14. Nielsen K., Lin M., Gall D., and Jolley M. Fluorescence polarization immunoassay: detection of antibody to Brucella abortus. Methods 22 (2000) 71-76
15. Tencza S.B., Islam K.R., Kalia V., Nasir M.S., Jolley M.E., and Montelaro R.C. Development of a fluorescence polarization-based diagnostic assay for equine infectious anemia virus. J. Clin. Microbiol. 38 (2000) 1854-1859
16. Nasir M.S., and Jolley M.E. Development of a fluorescence polarization assay for the determination of aflatoxins in grains. J. Agric. Food Chem. 50 (2002) 3116-3121
17. Burke T.J., Loniello K.R., Beebe J.A., and Ervin K.M. Development and application of fluorescence polarization assays in drug discovery. Comb. Chem. High Throughput Screen. 6 (2003) 183-194
18. Matayoshi E., and Swift K. Applications of FCS to protein-ligand interactions: comparison with fluorescence polarization. In: Scaefer F.P., Toennies J.P., and Zinth W. (Eds). Fluorescence Correlation Spectroscopy vol. 65 (2001), Springer, Berlin 85-98
19. Nasir M.S., and Jolley M.E. Fluorescence polarization: an analytical tool for immunoassay and drug discovery. Comb. Chem. High Throughput Screen. 2 (1999) 177-190
20. Jameson D.M., and Croney J.C. Fluorescence polarization: past, present, and future. Comb. Chem. High Throughput Screen. 6 (2003) 167-176
21. Carruthers N.J., and Stemmer P.M. Methionine oxidation in the calmodulin-binding domain of calcineurin disrupts calmodulin binding and calcineurin activation. Biochemistry 47 (2008) 3085-3095
22. Berridge M.J., Bootman M.D., and Roderick H.L. Calcium signalling: dynamics, homeostasis, and remodeling. Nat. Rev. Mol. Cell Biol. 4 (2003) 517-529
23. Anderson K.A., Noeldner P.K., Reece K., Wadzinski B.E., and Means A.R. Regulation and function of the calcium/calmodulin-dependent protein kinase IV/protein serine/threonine phosphatase 2a signaling complex. J. Biol. Chem. 279 (2004) 31708-31716
24. Chin D., and Means A.R. Calmodulin: a prototypical calcium sensor. Trends Cell Biol. 10 (2000) 322-328
25. Hoeflich K.P., and Ikura M. Calmodulin in action: diversity in target recognition and activation mechanisms. Cell 108 (2002) 739-742
26. Vetter S.W., and Leclerc E. Novel aspects of calmodulin target recognition and activation. Eur. J. Biochem. 270 (2003) 404-414
27. Crivici A., and Ikura M. Molecular and structural basis of target recognition by calmodulin. Annu. Rev. Biophys. Biomol. Struct. 24 (1995) 85-116
28. Kuboniwa H., Tjandra N., Grzesiek S., Ren H., Klee C.B., and Bax A. Solution structure of calcium-free calmodulin. Nat. Struct. Biol. 2 (1995) 768-776
29. Carafoli E. Intracellular calcium homeostasis. Annu. Rev. Biochem. 56 (1987) 395-433
30. 2+ pump. J. Membr. Biol. 165 (1998) 101-109
31. 2+-dependent ATPase of the sarcoplasmic reticulum. Annu. Rev. Biochem. 48 (1979) 275-292
32. 2+-ATPase from human red-cell membranes by calmodulin: ATP and partial proteolysis. Biochim. Biophys. Acta 858 (1986) 21-30
33. 2+ pump interacts both with calmodulin and with another part of the pump. J. Biol. Chem. 264 (1989) 12313-12321
34. Michaelis M.L., Bigelow D.J., Schöneich C., Williams T.D., Ramonda L., Yin D., Huhmer A.F.R., Yao Y., and Squier T.C. Decreased plasma membrane calcium transport activity in aging brain. Life Sci. 59 (1996) 405-412
35. Squier T.C., and Bigelow D.J. Protein oxidation and age-dependent alterations in calcium homeostasis. Front. Biosci. 5 (2000) d504-d526
36. 2+-ATPase by hydrogen peroxide and protection by calmodulin. Biochemistry 42 (2003) 12001-12010
37. 2+ pump is slow and is changed by alternative splicing. J. Biol. Chem. 274 (1999) 35227-35232
38. 2+ ATPase from human erythrocyte membranes. Arch. Biochem. Biophys. 210 (1981) 257-262
39. Allen M.W., Urbauer R.J.B., Zaidi A., Williams T.D., Urbauer J.L., and Johnson C.K. Fluorescence labeling, purification, and immobilization of a double cysteine mutant calmodulin fusion protein for single-molecule experiments. Anal. Biochem. 325 (2004) 273-284
40. 2+-ATPase. Biochemistry 44 (2005) 11074-11081
41. Peterson G.L. A simplification of the protein assay method of Lowry et al. which is more generally applicable. Anal. Biochem. 83 (1977) 346-356
42. Babu Y.S., Bugg C.E., and Cook W.J. Structure of calmodulin refined at 2.2 Å resolution. J. Mol. Biol. 204 (1988) 191-204
43. 2+-regulated activation by oligomerization. J. Biol. Chem. 264 (1989) 19495-19499
44. Rusinova E., Tretyachenko-Ladokhina V., Vele O.E., Senear D.F., and Alexander Ross J.B. Alexa and Oregon green dyes as fluorescence anisotropy probes for measuring protein-protein and protein-nucleic acid interactions. Anal. Biochem. 308 (2002) 18-25
45. Levi V., Rossi J.P., Castello P.R., and Gonzalez Flecha F.L. Structural significance of the plasma membrane calcium pump oligomerization. Biophys. J. 82 (2002) 437-446
46. Sun H., and Squier T.C. Ordered and cooperative binding of opposing globular domains of calmodulin to the plasma membrane Ca-ATPase. J. Biol. Chem. 275 (2000) 1731-1738
47. Yazawa M., Vorherr T., James P., Carafoli E., and Yagi K. Binding of calcium by calmodul. Influence of the calmodulin binding domain of the plasma membrane calcium pump. Biochemistry 31 (1992) 3171-3176
48. 2+ binding and implications for kinase activation. Protein Sci. 6 (1997) 794-807
49. Maulet Y., and Cox J.A. Structural changes in melittin and calmodulin upon complex formation and their modulation by calcium. Biochemistry 22 (1983) 5680-5686
50. Olwin B.B., and Storm D.R. Calcium binding to complexes of calmodulin and calmodulin binding proteins. Biochemistry 24 (1985) 8081-8086
51. Stemmer P.M., and Klee C.B. Dual calcium ion regulation of calcineurin by calmodulin and calcineurin B. Biochemistry 33 (1994) 6859-6866
52. Bayley P.M., Findlay W.A., and Martin S.R. Target recognition by calmodul. Dissecting the kinetics and affinity of interaction using short peptide sequences. Protein Sci. 5 (1996) 1215-1228
53. Linse S., Helmersson A., and Forsen S. Calcium binding to calmodulin and its globular domains. J. Biol. Chem. 266 (1991) 8050-8054
54. Sorensen B.R., and Shea M.A. Interactions between domains of apo calmodulin alter calcium binding and stability. Biochemistry 37 (1998) 4244-4253