Single-molecule characterization of the dynamics of calmodulin bound to oxidatively modified plasma-membrane Ca2+-ATPase

Biochemistry

Volumn 44, Issue 33, 2005, Pages 11074-11081

  Osborn, Kenneth D ^a   Zaidi, Asma ^a   Bieber Urbauer, Ramona J ^a,b   Michaelis, Mary L ^a   Johnson, Carey K ^a  

^a UNIVERSITY OF KANSAS   (United States)

^b University of Georgia ^*  (United States)

Author keywords

[No Author keywords available]

Indexed keywords

BINDING ENERGY; CONFORMATIONS; FLUORESCENCE; OXIDATION; PLASMAS; POLARIZATION; SPECTROSCOPIC ANALYSIS;

CALMODULIN; FLUORESCENCE SPECTROSCOPY; OXIDATIVE MODIFICATION; SINGLE-MOLECULE CHARACTERIZATION;

ENZYMES;

ADENOSINE TRIPHOSPHATASE (CALCIUM); ADENOSINE TRIPHOSPHATE; CALCIUM ION; CALMODULIN; CHYMOTRYPSIN; FLUORESCENT DYE; MEMBRANE ENZYME; NUCLEOTIDE;

ARTICLE; BINDING AFFINITY; BINDING SITE; CALCIUM HOMEOSTASIS; CALCIUM TRANSPORT; CELL MEMBRANE; COMPLEX FORMATION; CONFORMATIONAL TRANSITION; CONTROLLED STUDY; DISSOCIATION; ENZYME ACTIVATION; ENZYME ACTIVE SITE; ENZYME ACTIVITY; ENZYME ASSAY; ENZYME INACTIVATION; ENZYME MODIFICATION; FLUORESCENCE SPECTROSCOPY; HUMAN; MOLECULAR DYNAMICS; NONHUMAN; OXIDATION; POLARIZATION MODULATION SPECTROSCOPY; PRIORITY JOURNAL; PROTEIN BINDING; PROTEIN CONFORMATION; PROTEIN DEGRADATION; PROTEIN DOMAIN; PROTEIN FUNCTION; PROTEIN PROTEIN INTERACTION; SIGNAL TRANSDUCTION; SPECTROSCOPY; STRUCTURE ANALYSIS;

ADENOSINE TRIPHOSPHATE; ANIMALS; CALCIUM; CALCIUM-TRANSPORTING ATPASES; CALMODULIN; CHICKENS; ERYTHROCYTE MEMBRANE; FLUORESCENT DYES; OXIDATION-REDUCTION; PROTEIN BINDING; PROTEIN CONFORMATION; PROTEIN STRUCTURE, QUATERNARY; PROTEIN STRUCTURE, TERTIARY; SPECTROMETRY, FLUORESCENCE;

EID: 23944485408     PISSN: 00062960     EISSN: None     Source Type: Journal    
DOI: 10.1021/bi050488m     Document Type: Article

References (32)

1. Carafoli, E. (1987) Intracellular Calcium Homeostasis, Annu. Rev. Biochem. 56, 395-433.
2. 2+ Pump, J. Membr. Biol. 165, 101-109.
3. 2+ Pump, J. Biol. Chem. 263, 14152-14159.
4. 2+ Pump Interacts both with Calmodulin and with Another Part of the Pump, J. Biol. Chem. 264, 12313-12321.
5. 2+ Pump Contains a Site That Interacts with Its Calmodulin-Binding Domain, J. Biol. Chem. 266, 2930-2936.
6. 2+ Pump. 28 Residues Suffice to Bind Calmodulin but More are Needed for Full Auto-Inhibition of the Activity. J. Biol. Chem. 269, 1687-1691.
7. Padanyi, R., Paszty, K., Penheiter, A. R., Filoteo, A. G., Penniston, J. T., and Enyedi, A. (2003) Intramolecular Interactions of the Regulatory Region with the Catalytic Core in the Plasma Membrane Calcium Pump, J. Biol. Chem. 278, 35798-35804.
8. 2+ Pump by Substitution of Aspartic 170 by Asparagin. Activation of Plasma Membrane Calcium ATPase 4 without Disruption of the Interaction Between the Catalytic Core and the C-terminal Regulatory Domain. J. Biol. Chem. 279, 41619-41625.
9. 2+ Pump of Plasma Membranes, CRC Press, Boca Raton, FL.
10. 2+-Dependent ATPase of the Sarcoplasmic Reticulum, Annu. Rev. Biochem, 48, 275-292.
11. Toyoshima, C., Nakasako, M., Nomura, H., and Ogawa, H. (2000) Crystal Structure of the Calcium Pump of Sarcoplasmic Reticulum at 2.6 Å Resolution, Nature 405, 647-655.
12. Toyoshima, C., and Nomura, H. (2002) Structural Changes in the Calcium Pump Accompanying the Dissociation of Calcium, Nature 418, 605-611.
13. Berridge, M. J., Lipp, P., and Bootman, M. D. (2000) The Versatility and Universality of Calcium Signaling, Nat. Rev. Mol. Cell Biol. 1, 11-21.
14. Sastry, P. S., and Rao, K. S. (2000) Apoptosis and the Nervous System, J. Neurochem. 74, 1-20.
15. Bootman, M. D., Berridge, M. J., and Roderick, H. L. (2002) Calcium Signalling: More Messengers, More Channels, More Complexity, Curr. Biol. 12, R563-R565.
16. 2+-ATPase, Free Radical Biol. Med. 27, 810-821.
17. 2+-ATPase by Hydrogen Peroxide and Protection by Calmodulin, Biochemistry 42, 12001-12010.
18. 2+-ATPase in F344/BNF1 Rats, Neurobiol. Aging 19, 487-495.
19. Xie, X. S., and Dunn, R. C. (1994) Probing Single Molecule Dynamics, Science 265, 361-364.
20. Ha, T., Laurence, T. A., Chemla, D. S., and Weiss, S. (1999) Polarization Spectroscopy of Single Fluorescent Molecules, J. Phys. Chem. B 103, 6839-6850.
21. Warshaw, D. M., Hayes, E., Gaffney, D., Lauzon, A. M., Wu, J., Kennedy, G., Trybus, K., Lowey, S., and Berger, C. (1998) Myosin Conformatioiial States Determined by Single Fluorophore Polarization, Proc. Natl. Acad. Sci. U.S.A. 95, 8034-8039.
22. Adachi, K., Yasuda, R., Noji, H., Itoh, H., Harada, Y., Yoshida, M., and Kinosita, K., Jr. (2000) Stepping Rotation of F1-ATPase Visualized Through Angle-resolved Single-fluorophore Imaging. Proc. Natl. Acad. Sci. U.S.A. 97, 7243-7247.
23. Sosa, H., Peterman, E. J., Moerner, W. E., and Goldstein, L. S. (2001) ADP-Induced Rocking of the Kinesin Motor Domain Revealed by Single-molecule Fluorescence Polarization Microscopy, Nat. Struct. Biol. 8, 540-544.
24. Forkey, J. N., Quinlan, M. E., Shaw, M. A., Corrie, J. E., and Goldman, Y. E. (2003) Three-dimensional Structural Dynamics of Myosin V by Single-molecule Fluorescence Polarization. Nature 422, 399-404.
25. Osborn, K. D., Singh, M. K., Urbauer, R. J. B., and Johnson, C. K. (2003) Maximum Likelihood Approach to Single-Molecule Polarization Modulation Analysis, ChemPhysChem 4, 1005-1011.
26. 2+-ATPase by Calmodulin, Biophys. J. 87, 1892-1899.
27. 2+-ATPase Bound to Oxidatively Modified Calmodulin, Biochemistry 43, 12937-12944.
28. Yao, Y., Gao, J., and Squier, T. C. (1996) Dynamic Structure of the Calmodulin-Binding Domain of the Plasma Membrane Ca-ATPase in Native Erythrocyte Ghost Membranes, Biochemistry 35, 12015-12028.
29. Allen, M. W., Urbauer, R. J. B., Zaidi, A., Williams, T. D., Urbauer, J. L., and Johnson, C. K. (2004) Fluorescence Labeling, Purification and Immobilization of a Double Cysteine Mutant Calmodulin Fusion Protein for Single-Molecule Experiments, Anal. Biochem. 325, 273-284.
30. Fabiato, A. (1988) Computer Programs For Calculating Total From Specified Free Or Free From Specified Total Ionic Concentrations In Aqueous Solutions Containing Multiple Metals And Ligands, Methods Enzymol. 157, 378-417.
31. Lanzetta, P. A., Alverez, L. J., Reinsch, P. S., and Candia, O. (1979) An Improved Assay for Nanomole Amounts of Inorganic Phosphate, Anal. Biochem. 100, 95-97.
32. 2+ Pump Interacts with the Transduction Domain of the Enzyme, Protein Sci. 1, 1613-1621.