Fluorescence labeling, purification, and immobilization of a double cysteine mutant calmodulin fusion protein for single-molecule experiments

Analytical Biochemistry

Volumn 325, Issue 2, 2004, Pages 273-284

  Allen, Michael W ^a   Urbauer, Ramona J Bieber ^b,c   Zaidi, Asma ^a   Williams, Todd D ^a   Urbauer, Jeffrey L ^b,c   Johnson, Carey K ^a  

^a UNIVERSITY OF KANSAS   (United States)

^b UNIVERSITY OF GEORGIA   (United States)

^c NONE   (United States)

Author keywords

Calmodulin; Fluorescent labeling; Fusion protein; Maltose binding protein; Single molecule spectroscopy; spFRET

Indexed keywords

AMINO ACIDS; BINDING ENERGY; CHEMICAL ANALYSIS; ENERGY TRANSFER; FORSTER RESONANCE ENERGY TRANSFER; GELS; MALTOSE; MASS SPECTROMETRY; MOLECULES;

FLUORESCENT LABELING; FUSION PROTEINS; MALTOSE BINDING PROTEINS; SINGLE MOLECULE SPECTROSCOPY; SPFRET;

CALMODULIN;

AGAROSE; CALMODULIN; CYSTEINE; FLUORESCENT DYE; HYBRID PROTEIN; MALTOSE BINDING PROTEIN; MUTANT PROTEIN;

ARTICLE; CONTROLLED STUDY; FLUORESCENCE RESONANCE ENERGY TRANSFER; FLUORESCENCE SPECTROSCOPY; MASS SPECTROMETRY; MOLECULAR BIOLOGY; MOLECULAR DYNAMICS; MOLECULAR WEIGHT; NONHUMAN; NORMAL DISTRIBUTION; PARTICLE SIZE; PRIORITY JOURNAL; PROTEIN IMMOBILIZATION; PROTEIN PROTEIN INTERACTION; PROTEIN PURIFICATION; PROTEIN STRUCTURE;

EID: 1642497604     PISSN: 00032697     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.ab.2003.10.045     Document Type: Article

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