Fluorescence labeling, purification, and immobilization of a double cysteine mutant calmodulin fusion protein for single-molecule experiments

Analytical Biochemistry

Volumn 325, Issue 2, 2004, Pages 273-284

  Allen, Michael W ^a   Urbauer, Ramona J Bieber ^b,c   Zaidi, Asma ^a   Williams, Todd D ^a   Urbauer, Jeffrey L ^b,c   Johnson, Carey K ^a  

^a UNIVERSITY OF KANSAS   (United States)

^b UNIVERSITY OF GEORGIA   (United States)

^c University of Georgia   (United States)

Author keywords

Calmodulin; Fluorescent labeling; Fusion protein; Maltose binding protein; Single molecule spectroscopy; spFRET

Indexed keywords

AMINO ACIDS; BINDING ENERGY; CHEMICAL ANALYSIS; ENERGY TRANSFER; FORSTER RESONANCE ENERGY TRANSFER; GELS; MALTOSE; MASS SPECTROMETRY; MOLECULES;

FLUORESCENT LABELING; FUSION PROTEINS; MALTOSE BINDING PROTEINS; SINGLE MOLECULE SPECTROSCOPY; SPFRET;

CALMODULIN;

AGAROSE; CALMODULIN; CYSTEINE; FLUORESCENT DYE; HYBRID PROTEIN; MALTOSE BINDING PROTEIN; MUTANT PROTEIN;

ARTICLE; CONTROLLED STUDY; FLUORESCENCE RESONANCE ENERGY TRANSFER; FLUORESCENCE SPECTROSCOPY; MASS SPECTROMETRY; MOLECULAR BIOLOGY; MOLECULAR DYNAMICS; MOLECULAR WEIGHT; NONHUMAN; NORMAL DISTRIBUTION; PARTICLE SIZE; PRIORITY JOURNAL; PROTEIN IMMOBILIZATION; PROTEIN PROTEIN INTERACTION; PROTEIN PURIFICATION; PROTEIN STRUCTURE;

EID: 1642497604     PISSN: 00032697     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.ab.2003.10.045     Document Type: Article

References (52)

1. Xie X.S., Dunn R.C. Science. 265:1994;361-364.
2. Nie S., Chiu D.T., Zare R.N. Science. 266:1994;1018-1021.
3. Xue Q., Yeung E.S. Nature. 373:1995;681-683.
4. Moerner W.E. Science. 277:1997;1059-1060.
5. Nie S., Zare R.N. Annu. Rev. Biophys. Biomol. Struct. 26:1997;567-596.
6. Moerner W.E., Orrit M. Science. 283:1999;1670-1676.
7. Keller R.A., Ambrose W.P., Arias A.A., Cai H., Emory S.R., Goodwin P.M., Jett J.H. Anal. Chem. 74:2002;316A-324A.
8. Lu H.P., Xun L., Xie X.S. Science. 282:1998;1877-1882.
9. Xie X.S., Trautman J.K. Annu. Rev. Phys. Chem. 49:1998;441-480.
10. Weiss S. Science. 283:1999;1676-1683.
11. Osborn K.D., Singh M.K., Urbauer R.J.B., Johnson C.K. Chem. Phys. Chem. 4:2003;1005-1011.
12. Cheung H.C. Topics Fluorescence Spectrosc. 2:1991;127-176.
13. Van Der Meer B.W., Coker III G., Chen S.S.Y. Resonance Energy Transfer: Theory and Data. 1994.
14. Ha T., Ting A.Y., Liang J., Caldwell W.B., Deniz A.A., Chemla D.S., Schultz P.G., Weiss S. Proc. Natl. Acad. Sci. USA. 96:1999;893-898.
15. Ishii Y., Yoshida T., Funatsu T., Wazawa T., Yanagida T. Chem. Phys. 247:1999;163-173.
16. Brasselet S., Peterman E.J.G., Miyawaki A., Moerner W.E. J. Phys. Chem. B. 104:2000;3676-3682.
17. Deniz A.A., Laurence T.A., Beligere G.S., Dahan M., Martin A.B., Chemla D.S., Dawson P.E., Schultz P.G., Weiss S. Proc. Natl. Acad. Sci. USA. 97:2000;5179-5184.
18. Talaga D.S., Lau W.L., Roder H., Tang J., Jia Y., DeGrado W.F., Hochstrasser R.M. Proc. Natl. Acad. Sci. USA. 97:2000;13021-13026.
19. Borsch M., Diez M., Zimmermann B., Reuter R., Graber P. FEBS Lett. 527:2002;147-152.
20. Schuler B., Lipman E.A., Eaton W.A. Nature. 419:2002;743-747.
21. Zhuang X., Kim H., Pereira M.J.B., Babcock H.P., Walter N.G., Chu S. Science. 296:2002;1473-1476.
22. Rhoades E., Gussakovsky E., Haran G. Proc. Natl. Acad. Sci. USA. 100:2003;3197-3202.
23. Kapanidis A.N., Weiss S. J. Chem. Phys. 117:2002;10953-10964.
24. Mekler V., Kortkhonjia E., Mukhopadhyay J., Knight J., Revyakin A., Kapanidis A.N., Niu W., Ebright Y.W., Levy R., Ebright R.H. Cell. 108:2002;599-614.
25. Mukhopadhyay J., Kapanidis A.N., Mekler V., Kortkhonjia E., Ebright Y.W., Ebright R.H. Cell. 106:2001;453-463.
26. Crivici A., Ikura M. Annu. Rev. Biophys. Biomol. Struct. 24:1995;85-116.
27. Trewhella J. Cell Calcium. 13:1992;377-390.
28. Vogel H.J. Biochem. Cell. Biol. 72:1994;357-376.
29. Chattopadhyaya R., Meador W.E., Means A.R., Quiocho F.A. J. Mol. Biol. 228:1992;1177-1192.
30. Meador W.E., Means A.R., Quiocho F.A. Science. 262:1993;1718-1721.
31. Török K., Tzortzopoulos A., Grabarek Z., Best S.L., Thorogate R. Biochemistry. 40:2001;14878-14890.
32. Pluen A., Netti P.A., Jain R.K., Berk D.A. Biophys. J. 77:1999;542-552.
33. Ha T., Laurence T.A., Chemla D.S., Weiss S. J. Phys. Chem. B. 103:1999;6839-6850.
34. Boukobza E., Sonnenfeld A., Haran G. J. Phys. Chem. B. 105:2001;12165-12170.
35. Chirico G., Cannone F., Beretta S., Diaspro A., Campanini B., Bettati S., Ruotolo R., Mozzarelli A. Protein Sci. 11:2002;1152-1161.
36. Hu D., Lu H.P. J. Phys. Chem. B. 107:2003;618-626.
37. Jia Y., Talaga D.S., Lau W.L., Lu H.S.M., DeGrado W.F., Hochstrasser R.M. Chem. Phys. 247:1999;69-83.
38. Terpe K. Appl. Microbiol. Biotechnol. 60:2003;523-533.
39. Sachdev D., Chirgwin J. Methods Enzymol. 326:2000;312-321.
40. A. Miyawaki, H. Mizuno, J. Llopis, R.Y. Tsien, K. Jalink, Calcium Signal., second ed. (2001) 3-16.
41. Miyawaki A., Llopis J., Helm R., McCaffery J.M., Adams J.A., Ikura M., Tsien R.Y. Nature. 388:1997;882-887.
42. Niggli V., Adunyah E.S., Carafoli E. J. Biol. Chem. 256:1981;8588-8592.
43. Hemsley A., Arnheim N., Toney M.D., Cortopassi G., Galas D.J. Nucleic Acids Res. 17:1989;6545-6551.
44. Weiner M.P., Costa G.L., Schoettlin W., Cline J., Mathur E., Bauer J.C. Gene. 151:1994;119-123.
45. Gopalakrishna R., Anderson W.B. Biochem. Biophys. Res. Commun. 104:1982;830-836.
46. Lanzetta P.A., Alvarez L.J., Reinach P.S., Candia O.A. Anal. Biochem. 100:1979;95-97.
47. Cheezum M.K., Walker W.F., Guilford W.H. Biophys. J. 81:2001;2378-2388.
48. Kellermann O.K., Ferenci T. Methods Enzymol. 90:1982;459-463.
49. H. Bedouelle, A. Blondel, F. Bregegere, P. England, R. Nageotte, P. Rondard, in: Perspectives on Protein Engineering & Complementary Technologies, Collected Papers, International Symposium, 3rd, Oxford, 13-17 September 1994, 1995, pp. 180-183.
50. Gill S.C., Von Hippel P.H. Anal. Biochem. 182:1989;319-326.
51. Enyedi A., Vorherr T., James P., McCormick D.J., Filoteo A.G., Carafoli E., Penniston J.T. J. Biol. Chem. 264:1989;12313-12321.
52. Rees D.A. Biochem. J. 126:1972;257-273.