Fluorescence polarization: An analytical tool for immunoassay and drug discovery

Combinatorial Chemistry and High Throughput Screening

Volumn 2, Issue 4, 1999, Pages 177-190

  Nasir, Mohammad Sarwar ^a   Jolley, Michael E ^a,b  

^a Diachemix Corporation   (United States)

^b Jolley Consulting and Research Inc   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

DIAGNOSTIC VALUE; DNA DETERMINATION; DRUG BLOOD LEVEL; DRUG DETERMINATION; DRUG ISOLATION; DRUG MONITORING; FLUORESCENCE POLARIZATION; IMMUNOASSAY; PRIORITY JOURNAL; PROTEIN PROTEIN INTERACTION; REVIEW; SUBSTANCE ABUSE; TOXIN ANALYSIS;

ANIMALS; CHEMISTRY; COMMUNICABLE DISEASES; FLUORESCENCE POLARIZATION; HUMANS; IMMUNOASSAY;

ANIMALIA;

EID: 0032861406     PISSN: 13862073     EISSN: None     Source Type: Journal    
DOI: None     Document Type: Review

References (98)

1. Perrin, M. F. "Polarization de la lumiere de fluorescence. Vie moyenne de molecules dans l'etat excite." J. Phys. Radium, 1926, 7, 390-401.
2. Jameson, D. M.; Sawyer, W. H. " Fluorescence anisotropy applied to biomolecular interactions." Methods. Enzymol. 1995, 246, 283-300.
3. Hill, J. J.; Royer, C. A. "Fluorescence approaches to study of Protein-Nucleic acid complexation." Methods. Enzymol. 1997, 278, 390-416.
4. Ozinskas, A. J.; Malak, H.; Joshi, J.; Szmacinski, H.; Britz, J.; Thompson, R. B.; Koen, P. A.; Lakowicz, J. R. " Homogeneous model immunoasay of thyroxine by phase-modulation fluorescence spectroscopy." Anal. Biochem. 1993, 213, 264-270.
5. Checovich, W. J.; Bolger, R. E.; Burke, T. "Fluorescence polarization-a new tool for cell and molecular biology." Nature, 1995, 375, 254-256.
6. Weber, G. "Rotational Brownian motion and polarization of the fluorescence of solutions." Adv. Protein. Chem. 1953, 8, 415-59.
7. Weber, G. J. Opt. Soc. Am. 1956, 46, 962.
8. Weber, G. in "Fluorescence and Phosphorescence Analysis." (D. M. Hercules, Ed.) 1966, 217, Wiley, NY.
9. Weber, G. "Polarization of the fluorescence of macromolecules. 1: Theory and experimental method." Biochem. J. 1951, 51, 145-155.
10. Weber, G. "Polarization of the fluorescence of macromolecules. 2: Fluorescent conjugates of ovalbumin and bovine serum albumin." Ibid, 155-167.
11. Laurence, D. J. R. "A study of the adsorption of dyes on bovine serum albumin by the method of polarization of fluorescence." Biochem. J. 1951, 51, 168-180.
12. Dandliker, W. B.; Feigen, G. A. "Quantification of the antigen-antibody reaction by the polarization of fluorescence." Biochem. Biophys. Res. Commun. 1961, 5, 299-304.
13. Dandliker, W. B.; de Saussure, V. A. "Fluorescence polarization in immunochemistry." Immunochem. 1970, 7, 799.
14. Dandliker, W. B.; Kelly, R. J.; Dandliker, J.; Farquhar, J.; Levim, J. "Fluorescence Polarization immunoassay. Theory and experimental method." Immunochem. 1973, 10, 219-227.
15. Levison, S. A.; Dandliker, W. B.; Brawn, R. J.; Vanderlaan, W. P. "Fluorescence polarization measurement of the hormone-binding site interactions." Endocr. 1976, 99, 1129-1143.
16. Jolley, M. E. "Fluorescence polarization immunoassay for the determination of therapeutic drug levels in human plasma." J. Anal. Toxicol. 1981, 5, 236-240.
17. Jolley, M. E.; Stroupe, S. D.; Schwenzer, K. S.; Wang, C. J.; Lu-Steffes, M.; Hill, H. D.; Popelka, S. R.; Holen, J. T.; Kelso, D. M. "Fluorescence polarization immunoassay . An automated system for therapeutic drug determination." Clin. Chem. 1981, 27, 1575-1579.
18. Jolley, M. E.; Stroupe, S. D.; Wang, C. J.; Panas, H. N.; Keegan, C. L.; Schmidt, R. L.; Schwenzer, K. S. "Fluorescence polarization immunoassay 1. Monitoring aminoglycoside antibiotics in serum and plasma." Clin. Chem. 1981, 27, 1190-1197.
19. Wu, P.; Brasseur, M.; Schindler, U. "A high-throughput STAT binding assay using fluorescence polarization." Anal. Biochem. 1997, 249, 29-36.
20. Sportsman, J. R. in "Cost-effective strategies for automated and accelerated high throughput screening ." IBC Medical Library series, 1996, pp. 1.8.1-1.8.22.
21. Jolley, M. E. "Fluorescence polarization assays for the detection of proteases and their inhibitors." J. Biomol. Screening, 1996, 1, 33-38.
22. Lynch, B. A.; Loiacono, K. A.; Tiong, C. L.; Adams, S. E.; MacNeil, I. A. "A fluorescence polarization based Src-SH2 binding assay." Anal. Biochem. 1997, 247, 77-82.
23. Spencer, R. D.; Toledo, F. B.; Williams, B. T.; Yoss, N. L. Clin. Chem. 1973, 92, 222-227.
24. Lu-Steffes, M.; Jolley, M.; Pittluck, G.; Panas, H.; Olive, D.; Wang, C. H. J.; Nystrom, D.; Stroupe, S. "Fluorescence polarization Immunoassays of phenytoin and phenobarbital." Clin. Chem. 1981, 27, 1093.
25. Haber, E.; Bennett, J. C. "Polarization of fluorescence as a measure of antigen-antibody interaction." Proc. Natl. Acad. Sci. USA, 1962, 48, 1935-1942.
26. Stevens, F. "Modification of an ELISA-based procedure for affinity determination: Correction necessary for use with bivalent antibody." J. Mol.Immunol. 1987, 24, 1055-1060.
27. Friguet, B.; Chaffotte, A. F.; Djavadi, O. L.; Goldberg, M. E. J. Immunol. Methods, 1985, 77, 305-319.
28. Hogg, P. J.; Johnston, S. C.; Bowles, M. R.; Pond, S. M.; Winzor, D. "Evaluation of equilibrium constants for antigen-antibody interactions by solid phase immunoassay: The binding of paraquat to its elicited mouse monoclonal antibody." J. Mol. Immunol. 1987, 24, 797-801.
29. Jiskoot, W.; Hoogerhout, P.; Beuvery, E. C.; Herron, J. N.; Crommelin, D. J. A. "Preparation and application of a fluorescein-labeled peptide for determining the affinity constant of a monoclonal antibody-hapten complex by fluorescence polarization." Anal. Biochem. 1991, 196, 421-426.
30. Harper, M.; Lema, F.; Boulot, G.; Poljak, R. "Antigen specificity and cross reactivity of monoclonal anti-lysozyme antibodies." J. Mol. Immunol. 1987, 24, 97-108.
31. Whitaker, T.; Horwitz, W.; Albert, R.; Neshem, S. " Variability associated with analytical methods used to measure aflatoxin in agricultural commodities." J. AOAC. Int. 1996, 79, 476-485.
32. Maune, C. "Vomitoxin troubles: is your mycotoxin control program good enough?" Pet food industry, Nov-Dec, 1995, 4-10.
33. Sydenham, E. W.; Shephard, G. S.; Thiel, P. G.; Bird, C.; Miller, B. M. "Determination of fumonisins in corn: Evaluation of competitive immunoassay and HPLC." J. Agri. Food Chem. 1996, 44, 159-164.
34. Eremin, S. A. "Polarization Fluoroimmunoassay for rapid, specific detection of pesticides." In ACS Symposium series No. 586. Immunoanalysis of Agrochemicals: Emerging Technologies. Eds. J. O. Nelson, A. E. Karu, R. Wong. Washington, D. C. 1995, 223-234.
35. Choi, M. J.; Choi, J.; Park, J.; Eremin, S. A. "Localization of the epitope in methamphetamine and its antibody use for the detection of methamphetamine by Polarization Fluoroimmunoassay." J. Immunoassay. 1995, 16, 263-278.
36. White, L. O.; Holt, H, A.; Reeves, D. S.; MacGowan, A. P. "Evaluation of innofluor fluorescence polarization immunoassay kits for the determination of serum concentrations of gentamicin, tobramycin, amikacin and vancomycin." J. Antimicrobial Chemotherapy, 1997, 39, 355-361.
37. Dasgupta, A.; Hart, A. P. "Rapid detection of oleander poisoning using fluorescence polarization immunoassay for digitoxin." Am. J. Clin. Pathol., 1997, 108, 411-416.
38. Jortani, S. A.; Helm, R. A.; Valdes, R. "Inhibition of Na, K-ATPase by oleandrin and oleandrigenin and their detection by digoxin immunoassays." Clin. Chem. 1996, 42, 1654-1458.
39. Rogers, K. R.; Kohl, S. D.; Riddick, L. A.; Glass, T. "Detection of 2,4-dichloro-phenoxyacetic acid using a fluorescence immunoanalyzer." Analyst. 1997, 122, 1107-1111
40. Wei, A-P.; Herron, J. N. "Use of synthetic peptides as tracer antigens in fluorescence polarization immunoassays of high molecular weight analytes." Anal. Chem. 1993, 65, 3372-3377.
41. Lin, M.; Sugden, E. A.; Jolley, M. E.; Stilwell, K. "Modification of the mycobacterium bovis extracellular protein MPB70 with fluorescein for rapid detection of specific serum antibodies by fluorescence polarization." Clin. Diagnos. Lab. Immun. 1996, 3, 438-443.
42. Nielsen, K.; Gall, D.; Jolley, M.; Leishman, G.; Balsevicius, S.; Smith, P.; Nicoletti, P.; Thomas, F. "A homogeneous fluorescence polarization assay for detection of antibody to Brucella abortus." J. Immunol. Methods. 1996, 195, 161-168.
43. Kobayashi, Y.; Amitani, K.; Watanabe, F.; Miyai, K. "Fluorescence Polarization immunoassay for cortisol." Clin. Chim. Acta. 1979, 92, 241-247.
44. Adamczyk, M.; Chen, Y-Y.; Johnson, D. D.; Reddy, R. E. "A stereoselective synthesis of 1a-(3′-carboxypropyl)-4-androsten-17b-ol-3-one: Preparation of immunoreagents for quantification of testosterone by fluorescence polarization immunoassay." Tetrahedron, 1997, 53, 12855-12866.
45. Choi, M. J.; Choi, J.; Yoon, D. Y.; Park, J.; Eremin, S. A. "Fluorescence polarization immunoassay of progesterone." Biol. Pharm. Bull. 1997, 20, 309-314.
46. Al-Ansari, A. A. K.; Smith, D. D.; Landon, J. "Polarization fluoroimmunoassay of serum and salivary cortisol." J. Steroid Biochem. 1983, 19, 1475-1480
47. Czernik, P. J.; Shin, D. S.; Hurlburt, B. K. "Functional selection and characterization of DNA binding sites for trp repressor of Escherichia coli." J. Biol. Chem. 1994, 269, 27869.
48. LeTilly, V.; Royer, C. A. "Fluorescence anisotropy assays implicate protein-protein interactions in regulating trp repressor DNA binding." Biochemistry, 1993, 32, 7753-7758.
49. Kwok, R. P. S.; Lundblad, J. R.; Chrivia, J. C.; Richards, J. P.; Bachinger, H. P.; Brennan, R. G.; Roberts, S. G. E.; Green, M. R.; Goodman, R. H. "Nuclear protein CBP is a coactivator for the transcription factor CREB." Nature, 1994, 370, 223-226.
50. Teichberg, V. I.; Shinitsky, M. "Fluorescence polarization studies of lysozyme and lysozyme-saccharide complexes." J. Mol. Biol. 1973, 74, 519.
51. Barinaga, M. "Designing therapies that target tumor blood vessels." Research News, Science, 1997, 275, 482-484.
52. Beckett, R. P.; Davidson, A. H.; Drummond, A. H.; Huxley, P.; Whittaker, M. "Recent advances in matrix metalloproteinase inhibitor research." DDT, 1996, 1, 16-26.
53. Bickett, D. M.; Green, M. D.; Berman, J.; Dezube, M.; Howe, A. S.; Brown, P.; Roth, J. T.; McGeehan, G. M. "A high throughput fluorogenic substrate for interstitial collagenase (MMP-1) and gelatinase (MMP-9)." Anal. Biochem. 1993, 212, 58-64.
54. Gowravaram, M. R.; Tomczuk, B. E.; Johnson, J. S.; Delecki, D.; Cook, E. R.; Ghose, A. K.; Matheowetz, A. M.; Spurlino, J. C.; Rubin, B.; Smith, D.; Pulvino, T.; Wahl, R. C. "Inhibition of matrix metalloproteinases by hydroxamates containing heteroatom-based modifications of the P1′ group." J. Med. Chem. 1995, 38, 2570-2581.
55. Maeda, H. "Assay of proteolytic enzymes by the fluorescence polarization technique." Anal. Biochem. 1979, 92, 222-227.
56. Levine, L. M.; Michener, M. L.; Toth, M. V.; Holverda, B. C. "Measurement of specific protease activity utilizing fluorescence polarization." Anal. Biochem. 1997, 247, 83-88.
57. Bolger, R. R.; Checovich, W. Biotechniques, 1994, 17, 585-589.
58. Hofman, M.; Shaffar, M. "Fluorescence depolarization assay for quantifying a-amylase in serum and urine." Clin. Chem. 1985, 31, 1478-1480.
59. Schade, S. Z.; Jolley, M. E.; Sarauer, B. J.; Simonson, L. G. "Spirochete protease detection by fluorescence polarization." Poster 1168, Am. Assoc. Dent. Res. Annul Meeting. 1994.
60. Schade, S. Z.; Jolley, M. E.; Sarauer, B. J.; Simonson, L. G. "BODIPY-α-Casein, a pH-independent protein substrate for protease assays using fluorescence polarization." Anal. Biochem. 1996, 243, 1-7.
61. Yonemura, K.; Maeda, H. "A new assay method for DNase by fluorescence polarization and fluorescence intensity using DNA-Ethidium bromide complex as a sensitive substrate." J. Biochem. 1982, 92, 1297-1303.
62. Bolger, R.; Thompson, D. Am. Biotech. Lab. 1994, 12, 113-116.
63. Lynch, B. A.; Loiacono, K. A.; Tiong, C. L.; Adams, S. E.; MacNeil, I. A. "A fluorescence polarization based Src-SH2 binding assay." Anal. Biochem. 1997, 247, 77-82.
64. Seethala, R.; Menzel, R. "A fluorescence polarization compitition immunoassay for tyrosine kinases." Anal. Biochem. 1998, 255, 257-262.
65. Seethala, R.; Menzel, R. " A homogeneous, fluorescence polarization assay for Src-family tyrosine kinases." Anal. Biochem. 1997, 253, 210-218.
66. Tang, Y-W.; Procop, G. W.; Persing, D. H. "Molecular diagnostics for infectious diseases." Clin. Chem. 1997, 43, 2021-2038.
67. Lewis, J. S.; Fakilo, O.; Foss, E.; Legarza, G.; Leskeys, A.; Lowe, K.; Powning, D. "Direct DNA probe assay for Neisseria gonorrhoeae in pharyngeal and rectal specimens." J. Clinic. Microbiol. 1993, 31, 2783-2785.
68. Drake, T. A.; Hindler, J. A.; Berlin, O. G.; Brukner, D. A. "Rapid identification of Mycobacterium avium complex in culture using DNA probes." J. Clin. Microbiol. 1987, 25, 1442-1445
69. Spargo, C. A.; Haaland, P. D.; Jurgensen, S. R.; Shank, D. D.; Walker, G. T. Mol. Cell. Probes., 1993, 7, 395-404.
70. Singer, V. L.; Jones, L. J.; Yue, S. T.; Haugland, R. P. "Characterization of picogreen reagent and development of a fluorescence-based solution assay for double-stranded DNA quantitation." Anal. Biochem. 1997, 249, 228-238.
71. Domeika, M.; Bassiri, M.; Mardh, P. A. "Diagnosis of genital chlamydia trachomatis infections in asymptomatic males by testing urine by PCR." J. Clin. Microbiol. 1994, 32, 2350-2352.
72. Bassiri, M.; Hu, H. Y.; Domeika, M. A.; Burczak, J.; Svensen, L. O.; Lee, H. H.; Mardh, P. A. "Detection of chlamydia trachomatis in urine specimens from women by ligase chain reaction." J. Clin. Microbiol. 1995, 33, 898-900.
73. Findlay, J. B.; Atwood, S. M.; Bergmeyer, L.; Chemelli, J.; Christy, K.; Cummins, T.; Donish, W.; Ekeze, T.; Flavo, J.; Patterson, D.; Puskas, J.; Quinen, J.; Shah, J.; Sharkey, D.; Sutherland, J. W. H.; Sutton, R.; Warren, H.; Wellman, J. "Automated closed-vessel system for in vitro diagnostics based on polymerase chain reaction." Clin. Chem. 1993, 39, 1927-1933.
74. Cimino, G. D.; Metchette, K. C.; Tessman, J. W.; Hearst, J. E.; Issaacs, S. T. "Post-PCR sterilization: a method to control carryover contamination for the polymerase chain reaction." Nucleic Acids Res. 1991, 19, 99-107.
75. Mullis, K. B.; Faloona, F. A. "Specific synthesis of DNA in vitro via a polymerase-catalyzed reaction." Methods. Enzymol. 1987, 155, 335-350.
76. Mullis, K. B. "The unusual origin of the polymerase chain reaction." Sci. Am. 1990, 262, 56-65.
77. Guatelli, J. C.; Whitfield, K. M.; Kwoh, D. Y.; Barringer, K. J.; Richmond, D. D.; Gingeras, T. R. "Isothermal in vitro amplification of nucleic acids by multienzyme reaction modeled after retroviral replication." Proc. Natl. Acad. Sci. USA, 1990, 87, 1874-1878.
78. Compton, J. "Nucleic acid sequence-based amplification." Nature, 1991, 350, 91-92.
79. Chernesky, M. A.; Lee, H.; Schachter, J.; Burczak, J. D.; Stamme, W. E.; McCormack, W. M.; Quinn, T. C. "Diagnosis of Chlamydia trachomatis urethral infection in symptomatic and asymptomatic men by testing first-void urine in a ligase chain reaction assay." J. Infect. Dis. 1994, 170, 1308-1311.
80. Walker, G. T.; Little, M. C.; Nadeau, J. G.; Shank, D. D. "Isothermal, in vitro amplification of DNA by a restriction enzyme/DNA polymerase system." Proc. Natl. Acad. Sci. USA. 1992, 89, 392-396.
81. Herning, T.; Tamiya, E.; Karube, I. "Specific liquid DNA hybridization kinetics measured by fluorescence polarization." Anal. Chim. Acta. 1991, 244, 207-213.
82. Tsuruoka, M.; Yano, K.; Ikebukuro, K.; Nakayama, H.; Masuda, Y.; Karube, I. "Optimization of the rate of DNA hybridization and rapid detection of methicillin resistant Staphylococcus aureus DNA using fluorescence polarization." J. Biotech. 1996, 48, 201-208.
83. Gibson, N. J.; Gillard, H. L.; Whitcombe, D.; Ferrie, R. M.; Newton, C. R.; Little, S. "A homogeneous method for genotyping with fluorescence polarization." Clin. Chem. 1997, 43, 1336-1341.
84. Walker, G. T.; Fraiser, M. S.; Schram, J. L.; Little, M. C.; Nadeau, J. G.; Malinowski, D. P. "Strand displacement amplification: An isothermal, in vitro DNA amplification technique." Nucleic Acids Res. 1992, 20, 1691-1696.
85. Kumke, M. U.; Li, G.; McGown, L. B.; Walker, G. T.; Linn, C. P. "Hybridization of fluorescent-labeled DNA oligomers detected by fluorescence anisotropy with protein binding enhancement." Anal. Chem. 1995, 67, 3945-3951.
86. Walker, G. T.; Linn, C. P. "Detection of mycobacterium tuberculosis DNA with thermophilic strand displacement amplification and fluorescence polarization." Clinical Chem. 1996, 42, 1604-1608
87. Spears, P. A.; Linn, C. P.; Woodard, D. L.; Walker, G. T. "Simultaneous strand displacement amplification and fluorescence polarization detection of Chlamydia trachomatis DNA." Anal. Biochem. 1997, 247, 130-137.
88. Terpetschnig, E.; Szmacinski, H.; Lakowicz, J. R. "Fluorescence polarization immunoassay of a high molecular weight antigen using a long wavelength absorbing and laser diode-excitable metal-ligand complex." Anal. Biochem. 1996, 240, 54-59.
89. Terpetschnig, E.; Lakowicz, J. R.; Szmacinski, H.; Malak, H. "Metal-ligand complexes as a new class of long lived fluorophores for protein hydrodynamics." Biophys. J. 1995, 68, 342-350.
90. Terpetschnig, E.; Szmacinski, H.; Lakowicz, J. R. "Fluorescence polarization immunoassay of a high molecular weight antigen based on a long lifetime Ru-ligand complex." Anal. Biochem. 1995, 227, 140-147.
91. Guo, X-Q.; Castellano, F. N.; Lakowicz, J. R. "Use of a long-lifetime Re(I) complex in fluorescence polarization immunoassays of high-molecular-weight analytes." Anal. Chem. 1998, 70, 632-637.
92. Kneas, K. A.; Xu, W.; Demas, J. N.; DeGraff, B. A. J. Chem. Edu. 1997, 74, 696.
93. Urios, P.; Cittanova, N. " Adaptation of fluorescence polarization immunoassay to the assay of macromolecules." Anal. Biochem. 1990, 185, 308-312.
94. Jolley, M. E. Unpublished observations.
95. 50) of an enzymatic reaction." Biochemical Pharmacology, 1973, 22, 3099-3108.
96. Munsen, P. J.; Rodbard, D. "An exact correction to the "Cheng-Prusoff" correction." J. Receptor Res., 1988, 8, 533-546.
97. Dandliker, W. B.; Hsu, M-L.; Levin, J.; Rao, B. R. "Equilibrium and kinetic inhibition assays based upon fluorescence polarization." Methods Enzymol. 1981, 74, 3-28.
98. th Ed., Blackwell, Oxford, UK. 1986, 25.1-25.30.