Novel preparation protocol for the expression and purification of recombinant staphylokinase

Biotechnology and Applied Biochemistry

Volumn 51, Issue 1, 2008, Pages 9-13

  Ren, Dongsheng ^a   Li, Dehua ^b   Yang, Weihua ^b   Li, Yun ^b   Gou, Xinghua ^b   Liang, Bo ^b   Li, Bogang ^a,b   Wu, Qiaqing ^a,b  

^a CHENGDU INSTITUTE OF BIOLOGY   (China)

^b Chengdu Di Ao Pharmaceutical Group   (China)

Author keywords

Escherichia coli; Expression; Ni2+ nitrilotriacetate; Purification; Q sepharose fast flow chromatography; Recombinant staphylokinase

Indexed keywords

COMPUTER NETWORKS;

EXPRESSION; NI2+-NITRILOTRIACETATE; PURIFICATION; Q-SEPHAROSE FAST FLOW CHROMATOGRAPHY; RECOMBINANT STAPHYLOKINASE;

ESCHERICHIA COLI;

HYBRID PROTEIN; NICKEL; PLASMIN; PLASMINOGEN; RECOMBINANT ENZYME; SEPHAROSE; STAPHYLOKINASE;

AFFINITY CHROMATOGRAPHY; AMINO TERMINAL SEQUENCE; ARTICLE; BACTERIAL GENE; BLOOD CLOT LYSIS; ENZYME ACTIVITY; ENZYME PURIFICATION; ESCHERICHIA COLI; EXPRESSION VECTOR; GENE EXPRESSION SYSTEM; HIGH PERFORMANCE LIQUID CHROMATOGRAPHY; NONHUMAN; POLYACRYLAMIDE GEL ELECTROPHORESIS; PROTEIN EXPRESSION; SAK GENE; STAPHYLOCOCCUS AUREUS; ULTRAFILTRATION;

BASE SEQUENCE; CHROMATOGRAPHY, AFFINITY; CHROMATOGRAPHY, HIGH PRESSURE LIQUID; CHROMATOGRAPHY, ION EXCHANGE; ELECTROPHORESIS, POLYACRYLAMIDE GEL; ESCHERICHIA COLI; GENE EXPRESSION REGULATION, BACTERIAL; METALLOENDOPEPTIDASES; MOLECULAR SEQUENCE DATA; POLYMERASE CHAIN REACTION; RECOMBINANT PROTEINS; STAPHYLOCOCCUS AUREUS; ULTRAFILTRATION;

ESCHERICHIA COLI; STAPHYLOCOCCUS AUREUS;

EID: 51449091043     PISSN: 08854513     EISSN: None     Source Type: Journal    
DOI: 10.1042/BA20070225     Document Type: Article

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