Development of a fed-batch process for the production of anticancer drug TATm-survivin(T34A) in Escherichia coli

Biochemical Engineering Journal

Volumn 43, Issue 2, 2009, Pages 163-168

  Zhang, Haiyi ^a   Zheng, Yu ^a   Liu, Qinghai ^a   Tao, Xinyi ^a   Zheng, Wenyun ^a   Ma, Xingyuan ^a   Wei, Dongzhi ^a  

^a EAST CHINA UNIVERSITY OF SCIENCE AND TECHNOLOGY   (China)

Author keywords

Acetic acid; Bioprocess design; Escherichia coli; Fed batch culture; Optimization; TATm survivin(T34A)

Indexed keywords

ACETIC ACID; AMMONIUM COMPOUNDS; BATCH CELL CULTURE; ESCHERICHIA COLI; GLUCOSE; OPTIMIZATION; PH; PH EFFECTS;

AMMONIUM CONCENTRATIONS; ANTI-CANCER DRUGS; BIOPROCESS DESIGN; CELLULAR PROTEINS; FED-BATCH CULTURE; INORGANIC NITROGENS; INTRACELLULAR PRODUCTIONS; PLASMID STABILITIES; POINT ADDITIONS; PRODUCTION LEVELS; RECOMBINANT PROTEIN EXPRESSIONS; TARGET GENES; TATM-SURVIVIN(T34A);

BATCH DATA PROCESSING;

ACETIC ACID; AMMONIA; ANTINEOPLASTIC AGENT; CELL PROTEIN; GLUCOSE; ISOPROPYL THIOGALACTOSIDE; NITROGEN; RECOMBINANT PROTEIN; T 34A; TATM SURVIVIN; UNCLASSIFIED DRUG;

ARTICLE; BACTERIAL CELL; CELL GROWTH; CONTROLLED STUDY; DRUG SYNTHESIS; ESCHERICHIA COLI; FED BATCH FERMENTATION; GENE EXPRESSION; GENE TARGETING; INTRACELLULAR SPACE; NONHUMAN; PLASMID; PRIORITY JOURNAL; PROMOTER REGION; PROTEIN EXPRESSION;

ESCHERICHIA COLI;

EID: 57649240432     PISSN: 1369703X     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.bej.2008.09.013     Document Type: Article

References (40)

1. Choi J.H., and Lee S.Y. Secretory and extracellular production of recombinant proteins using Escherichia coli. Appl. Microbiol. Biotechnol. 64 (2004) 625-635
2. Makrides S.C. Strategies for achieving high-level expression of genes in Escherichia coli. Microbiol. Rev. 60 (1996) 512-538
3. Vidal L., Álvaro G., Benaiges M.D., and Caminal G. Influence of induction and operation mode on recombinant rhamnulose-phosphate aldolase production by Escherichia coli using the T5 promoter. J. Biotechnol. 118 (2005) 75-87
4. Shin C.S., Hong M.S., Bae C.S., and Lee J. Enhanced Production of Human Mini-Proinsulin in Fed-Batch Cultures at High Cell Density of Escherichia coli BL21(DE3)[pET-3aT2M2]. Biotechnol. Prog. 13 (1997) 249-257
5. Austin C.J.D., Mizdrak J., Matin A., Sirijovski N., Kosim-Satyaputra P., Willows R.D., Roberts T.H., Truscott R.J.W., Polekhina G., Parker M.W., and Jamie J.F. Optimised expression and purification of recombinant human indoleamine 2,3-dioxygenase. Protein Expr. Purif. 37 (2004) 392-398
6. Fass R., van de Walle M., Shiloach A., Joslyn A., Kaufman J., and Shiloach J. Use of high density cultures of Escherichia coli for high level production of recombinant Pseudomonas aeruginosa exotoxin A. Appl. Microbiol. Biotechnol. 36 (1991) 65-69
7. Hellmuth K., Korz D.J., Sanders E.A., and Deckwer W.D. Effect of growth rate on stability and gene expression of recombinant plasmids during continuous and high cell density cultivation of Escherichia coli TG1. J. Biotechnol. 32 (1994) 289-298
8. Hu S.Y., Wu J.L., and Huang J.H. Production of tilapia insulin-like growth factor-2 in high cell density cultures of recombinant Escherichia coli. J. Biotechnol. 107 (2004) 161-171
9. Panda A.K., Ghorpade A., Mukhopadhyay A., Talwar G.P., and Garg L.C. High cell density fermentation of recombinant Vibrio cholerae for the production of B subunit of Escherichia coli enterotoxin. Biotechnol. Bioeng. 45 (1995) 245-250
10. Ramírez D.M., and Bentley W.E. Fed-batch feeding and induction policies that improve foreign protein synthesis and stability by avoiding stress responses. Biotechnol. Bioeng. 47 (1995) 596-608
11. Striedner G., Cserjan-Puschmann M., Potschacher F., and Bayer K. Tuning the transcription rate of recombinant protein in strong Escherichia coli expression systems through repressor titration. Biotechnol. Prog. 19 (2003) 1427-1432
12. Wong H.H., Kim Y.C., Lee S.Y., and Chang H.N. Effect of post-induction nutrient feeding strategies on the production of bioadhesive protein in Escherichia coli. Biotechnol. Bioeng. 60 (1998) 271-276
13. Ramalingam S., Gautam P., Mukherjee K.J., and Jayaraman G. Effects of post-induction feed strategies on secretory production of recombinant streptokinase in Escherichia coli. Biochem. Eng. J. 33 (2007) 34-41
14. Luli G.W., and Strohl W.R. Comparison of growth, acetate production and acetate inhibition of Escherichia coli strains in batch and fed-batch fermentations. Appl. Environ. Microbiol. 56 (1990) 1004-1011
15. Chen W., Graham C., and Ciccarelli R.B. Automated fed-batch fermentation with feed-back controls based on dissolved oxygen (DO) and pH for production of DNA vaccines. J. Ind. Microbiol. Biotechnol. 18 (1997) 43-48
16. Whiffin V.S., Cooney M.J., and Cord-Ruwisch R. Online detection of feed demand in high cell density cultures of Escherichia coli by measurement of changes in dissolved oxygen transients in complex media. Biotechnol. Bioeng. 85 (2004) 422-433
17. Johnston W.A., Stewart M., Lee P., and Cooney M.J. Tracking the acetate threshold using DO-transient control during medium and high cell density cultivation of recombinant Escherichia coli in complex media. Biotechnol. Bioeng. 84 (2003) 314-323
18. Johnston W., Cord-Ruwisch R., and Cooney M.J. Industrial control of recombinant E. coli fed-batch culture: new perspectives on traditional controlled variables. Bioprocess. Biosyst. Eng. 25 (2002) 111-120
19. Chen H.C., Hwang C.F., and Mou D.G. High-density Escherichia coli cultivation process for hyperexpression of recombinant porcine growth hormone. Enzyme Microb. Technol. 14 (1992) 321-326
20. Tsai L.B., Mann M., Morris F., Rotgers C., and Fenton D. The effect of organic nitrogen and glucose on the production of recombinant human insulin-like growth factor in high cell density Escherichia coli fermentations. J. Ind. Microbiol. 2 (1987) 181-187
21. Iding K., Buntemeyer H., Gudermann F., Deutschmann S.M., Kionka C., and Lehmann J. An automatic system for the assessment of complex medium additives under cultivation conditions. Biotechnol. Bioeng. 73 (2001) 442-448
22. Vila P., Corchero J.L., Benito A., and Villaverde A. Ammonium-mediated reduction of plasmid copy number and recombinant gene expression in Escherichia coli. Biotechnol. Prog. 10 (1994) 648-651
23. Ma X.Y., Zheng W.Y., Wei D.Z., Ma Y.S., Wang T.W., Wang J.Z., Liu Q.H., and Yang S.L. Construction, expression, and purification of HIV-TAT-survivin (T34A) mutant: A pro-apoptosis protein in Escherichia coli. Protein Expr. Purif. 47 (2006) 36-44
24. Ma X.Y., Zheng W.Y., Wei D.Z., Ma Y.S., Wang T.W., Wang J.Z., Liu Q.H., and Yang S.L. High-level expression, purification and pro-apoptosis activity of HIV-TAT-survivin (T34A) mutant to cancer cells in vitro. J. Biotechnol. 123 (2006) 367-378
25. Ngo T.T., Phan A.P.H., Yam C.F., and Lenhoff H.M. Interference in determination of ammonia with the hypochlorite-alkaline phenol method of Berthelot. Anal. Chem. 54 (1982) 46-49
26. Lowry O.H., Rosebrough N.J., Farr A.L., and Randall R.J. Protein measurement with the Folin phenol reagent. J. Biol. Chem. 193 (1951) 265-275
27. Lin H.Y., and Neubauer P. Influence of controlled glucose oscillations on a fed-batch process of recombinant Escherichia coli. J. Biotechnol. 79 (2000) 27-37
28. Madurawe R.D., Chase T.E., Tsao E.I., and Bentley W.E. A recombinant lipoprotein Antigen against lyme disease expressed in E. coli: fermentor operating strategies for improved yield. Biotechnol. Prog. 16 (2000) 571-576
29. Hoffmann F., Posten C., and Rinas U. Kinetic model of in vivo folding and inclusion body formation in recombinant Escherichia coli. Biotechnol. Bioeng. 72 (2001) 315-322
30. Hahm D.H., Kim S.H., Pan J., and Rhee J.S. Maximum Yield of Foreign Lipase in Escherichia coli HB101 Limited by Duration of Protein Expression. J. Ferment. Bioeng. 79 (1995) 236-241
31. Studier F.W. Protein production by auto-induction in highdensity shaking cultures. Protein Expr. Purif. 41 (2005) 207-234
32. Delcarte J., Fauconnier M.L., Jacques P., Matsui K., Thonart P., and Marlier M. Optimisation of expression and immobilized metal ion affinity chromatographic purification of recombinant (HiS)(6)-tagged cytochrome P450 hydroperoxide lyase in Escherichia coli. J. Chromatogr. B. 786 (2003) 229-236
33. Glick B.R. Metabolic load and heterologous gene-expression. Biotechnol. Adv. 13 (1995) 247-261
34. Bhattacharya S.K., and Dubey A.K. Metabolic burden as reflected by maintenance coefficient of recombinant Escherichia coli overexpressing target gene. Biotechnol. Lett. 17 (1995) 1155-1160
35. Hoffmann F., Weber J., and Rinas U. Metabolic adaptation of Escherichia coli during temperature-induced recombinant protein production. 1. Readjustment of metabolic enzyme synthesis. Biotechnol. Bioeng. 80 (2002) 313-319
36. Jensen E.B., and Carlsen S. Production of recombinant human growth hormone in Escherichia coli: expression of different precursors and physiological effects of glucose, acetate, and salts. Biotechnol. Bioeng. 36 (1990) 1-11
37. Doelle H.W., Ewings K.N., and Hollywood N.W. Regulation of glucose metabolism in bacterial systems. Adv. Biochem. Eng. 23 (1982) 1-35
38. EI-Mansi E.M.T., and Holms W.H. Control of carbon flux to acetate excretion during growth of Escherichia coli in batch and continuous cultures. J. Gen. Microbiol. 135 (1989) 2875-2883
39. Thompson B.G., Kole M., and Gerson D.F. Control of ammonium concentration in Escherichia coli fermentations. Biotechnol. Bioeng. 27 (1985) 818-824
40. Neidhardt F.C., Ingraham J.L., and Schaechter M. Physiology of the Bacterial Cell: A Molecular Approach (1990), Sinauer Associates, Sunderland, MA p. 3