Optimisation of expression and immobilized metal ion affinity chromatographic purification of recombinant (His)6-tagged cytochrome P450 hydroperoxide lyase in Escherichia coli

Journal of Chromatography B: Analytical Technologies in the Biomedical and Life Sciences

Volumn 786, Issue 1-2, 2003, Pages 229-236

  Delcarte, Jérôme ^a   Fauconnier, Marie Laure ^b   Jacques, Philippe ^b   Matsui, Kenji ^c   Thonart, Philippe ^b   Marlier, Michel ^b  

^a WALLOON AGRICULTURAL RESEARCH CENTRE   (Belgium)

^b UNIVERSITY OF LIÈGE   (Belgium)

^c YAMAGUCHI UNIVERSITY   (Japan)

Author keywords

Cytochrome P450; Escherichia coli; Expression; Histidine tag; Hydroperoxide lyase; Purification

Indexed keywords

CYTOLOGY; ESCHERICHIA COLI; FATTY ACIDS; PLANTS (BOTANY);

IMMOBILIZED METAL IONS;

AFFINITY CHROMATOGRAPHY;

CYTOCHROME P450; HISTIDINE; HYDROXIDE; LYASE; NICKEL;

BINDING AFFINITY; BIOREACTOR; CHROMATOGRAPHY; CONFERENCE PAPER; ENZYME PURIFICATION; ESCHERICHIA COLI; IMMOBILIZATION; NONHUMAN; PLANT; PRIORITY JOURNAL; PROTEIN EXPRESSION; PROTEIN PURIFICATION; TIME; ULTRAVIOLET SPECTROPHOTOMETRY;

ESCHERICHIA COLI;

EID: 0037465688     PISSN: 15700232     EISSN: None     Source Type: Journal    
DOI: 10.1016/S1570-0232(02)00815-2     Document Type: Conference Paper

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