An N-acetyl-β-d-glucosaminidase gene, cr-nag1, from the biocontrol agent Clonostachys rosea is up-regulated in antagonistic interactions with Fusarium culmorum

Mycological Research

Volumn 113, Issue 1, 2009, Pages 33-43

  Mamarabadi, Mojtaba ^a   Jensen, Dan Funck ^a   Lübeck, Mette ^a  

^a UNIVERSITY OF COPENHAGEN   (Denmark)

Author keywords

Biocontrol; Chitinase; Clonostachys rosea; Mycoparasitism; N acetyl d glucosaminidase; RT PCR

Indexed keywords

ACETYLGLUCOSAMINIDASE; FUNGAL PROTEIN;

ANTAGONISM; BIOASSAY; BIOCONTROL AGENT; ENZYME; FUNGUS; GENE; GENE EXPRESSION; GLUCOSE; PARASITE; PARASITISM; PATHOGEN;

AMINO ACID SEQUENCE; ANTIBIOSIS; ARTICLE; ASCOMYCETES; BIOLOGICAL PEST CONTROL; CLASSIFICATION; ENZYMOLOGY; FUSARIUM; GENE EXPRESSION REGULATION; GENETICS; GROWTH, DEVELOPMENT AND AGING; METABOLISM; MOLECULAR GENETICS; NUCLEOTIDE SEQUENCE; REVERSE TRANSCRIPTION POLYMERASE CHAIN REACTION; UPREGULATION;

ACETYLGLUCOSAMINIDASE; AMINO ACID SEQUENCE; ANTIBIOSIS; BASE SEQUENCE; FUNGAL PROTEINS; FUSARIUM; GENE EXPRESSION REGULATION, FUNGAL; HYPOCREALES; MOLECULAR SEQUENCE DATA; PEST CONTROL, BIOLOGICAL; REVERSE TRANSCRIPTASE POLYMERASE CHAIN REACTION; UP-REGULATION;

BIONECTRIA OCHROLEUCA; FUNGI; FUSARIUM CULMORUM; HEXAPODA; NEMATODA; PYTHIUM; PYTHIUM ULTIMUM;

EID: 57649240253     PISSN: 09537562     EISSN: 14698102     Source Type: Journal    
DOI: 10.1016/j.mycres.2008.07.005     Document Type: Article

References (48)

1. Altschul S.F., Gish W., Miller W., Myers E.W., and Lipman D.J. Basic local alignment search tool. Journal of Molecular Biology 215 (1990) 403-410
2. Ballance D.J. Sequences important for gene expression in filamentous fungi. Yeast 2 (1986) 229-236
3. Bendtsen J.D., Nielsen H., Heijne G.V., and Brunak S. Improved prediction of signal peptides: SignalP 3.0. Journal of Molecular Biology 340 (2004) 783-795
4. Benhamou N., and Chet I. Cellular and molecular mechanisms involved in the interaction between Trichoderma harzianum and Pythium ultimum. Applied Environtal Microbiology 63 (1997) 2095-2099
5. Brunner K., Peterbauer C.K., Mach R.L., Lorito M., Zeilinger S., and Kubicek C.K. The Nag1 N-acetylglucosaminidase of Trichoderma atroviride is essential for chitinase induction by chitin and of major relevance to biocontrol. Current Genetics 43 (2003) 289-295
6. Burge C., and Karlin S. Prediction of complete gene structures in human genomic DNA. Journal of Molecular Biology 268 (1997) 78-94
7. Cannon R.D., Niimi K., Jenkinson H.F., and Shepherd M.G. Molecular cloning and expression of the Candida albicans β-N-acetylglucosaminidase (HEX1) gene. Journal of Bacteriology 176 (1994) 2640-2647
8. Carsolio C., Gutierrez A., Jimenez B., van Montagu M., and Herrera-Estrella A. Characterization of ech-42, a Trichoderma harzianum endochitinase gene expressed during mycoparasitism. Proceedings of the National Academy of Sciences USA 91 (1994) 10903-10907
9. Davila M., Acosta N., Betancourt C., and Negron J. Chitinolytic capacity of fungi isolated from agricultural soils infested with the root-knot nematode (Meloidogyne spp.) in Puerto Rico. Journal of Agriculture of the University of Puerto Rico 83 (1999) 189-199
10. De las Mercedes Dana M., Limon M.C., Mejias R., Mach R.L., Benitez T., Pintor-Toro J.A., and Kubicek C.P. Regulation of chitinase 33 (chit33) gene expression in Trichoderma harzianum. Current Genetics 38 (2001) 335-342
11. Díez B., Rodríguez-Sáiz M., De la Fuente J.L., Moreno M., and Barredo J.L. The nag A gene of Penicillium chrysogenum encoding β-N-acetylglucosaminidase. FEMS Microbiology Letters 242 (2005) 257-264
12. Dugan F.M., Lupien S.L., Hernandez-Bello M., Peever T.L., and Chen W. Fungi resident in chickpea debris and their suppression of growth and reproduction of Didymella rabiei under laboratory conditions. Journal of Phytopathology 153 (2005) 431-439
13. Fang W., Leng B., Xiao Y., Jin K., Ma J., Fan Y., Feng J., Yang X., Zhang Y., and Pei Y. Cloning of Beauveria bassiana chitinase gene Bbchit1 and its application to improve fungal strain virulence. Applied Environmental Microbiology 71 (2005) 363-370
14. Goedegebuur F., Fowler T., Phillips J., van der Kley P., van Solingen P., Dankmeyer L., and Power S.D. Cloning and relational analysis of 15 novel fungal endoglucanases from family 12 glycosyl hydrolase. Current Genetics 41 (2002) 89-98
15. Higgins D., Thompson J., and Gibson T. CLUSTAL W: improving the sensitivity of progressive multiple sequence alignment through sequence weighting, position-specific gap penalties and weight matrix choice. Nucleic Acids Research 22 (1994) 4673-4680
16. Inbar J., and Chet I. The role of recognition in the induction of specific chitinases during mycoparasitism by Trichoderma harzianum. Microbiology 141 (1995) 2823-2829
17. Inglis G.D., and Kawchuk L.M. Comparative degradation of oomycete, ascomycete, and basidiomycete cell walls by mycoparasitic and biocontrol fungi. Canadian Journal of Microbiology 48 (2002) 60-70
18. Jensen B., Knudsen I.M.B., and Jensen D.F. Survival of conidia of Clonostachys rosea coated on barley seeds and their biocontrol efficacy against seed-borne Bipolaris sorokiniana. Biocontrol Science and Technology 12 (2002) 427-441
19. Jensen B., Knudsen I.M.B., Jensen D.F., and Hockenhull J. Biological seed treatment of cereals with fresh and long term stored formulations of Gliocladium roseum: Biocontrol efficacy against Fusarium culmorum. European Journal of Plant Pathology 106 (2000) 233-242
20. Jensen B., Knudsen I.M.B., Madsen M., and Jensen D.F. Biopriming of infected carrot seed with an antagonist, Clonostachys rosea, selected for control of seedborne Alternaria spp. Phytopathology 94 (2004) 551-560
21. Kim D.J., Baek J.M., Uribe P., Kenerley C.M., and Cook D.R. Cloning and characterization of multiple glycosyl hydrolase genes from Trichoderma virens. Current Genetics 40 (2002) 374-384
22. Knudsen I.M.B., Hockenhull J., and Jensen D.F. Biocontrol of seedling diseases caused by Fusarium culmorum and Bipolaris sorokiniana: effects of selected fungal antagonists on growth and yield components. Plant Pathology 44 (1995) 467-477
23. Knudsen I.M.B., Hockenhull J., Jensen D.F., Gerhardson B., Hökeberg M., Tahvonen R., Teperi E., Sundheim L., and Henriksen B. Selection of biological control agents for controlling soil- and seed-borne pathogens in the field. European Journal of Plant Pathology 103 (1997) 775-784
24. Knudsen I.M.B., Thomsen K.A., Jensen B., and Poulsen K.M. Effects of hot water treatment, biocontrol agents, disinfectants and a fungicide on storability of English oak acorns and control of the pathogen, Ciboria batschiana. Forest Pathology 34 (2004) 47-64
25. Lahoz E., Contillo R., and Porrone F. Induction of systemic resistance to Erysiphe orontii cast in tobacco by application on roots of an isolate of Gliocladium roseum Bainier. Journal of Phytopathology 152 (2004) 465-470
26. Latgé J.P. The cell wall: a carbohydrate armour for the fungal cell. Molecular Microbiology 66 (2007) 279-290
27. Lorito M. Chitinolytic enzymes and their genes. In: Harman G.E., and Kubicek C.P. (Eds). Trichoderma and Gliocladium. Enzymes, Biological Control and Commercial Applications vol. 2 (1998), Taylor and Francis, London 73-99
28. Lorito M., Harman G.E., Hayes C.K., Broadway R.M., Tronsmo A., Woo S.L., and Di Pietro A. Chitinolytic enzymes of Trichoderma harzianum: antifungal activity of purified endochitinase and chitobiosidase. Phytopathology 83 (1993) 302-307
29. Lübeck M., Knudsen I.M.B., Jensen B., Thrane U., Janvier C., and Jensen D.F. GUS and GFP transformation of the biocontrol strain Clonostachys rosea IK726 and the use of these marker genes in ecological studies. Mycological Research 106 (2002) 815-826
30. Luongo L., Galli M., Coraz L., Meekes E., De Haas L., Plas L.V., and Köhl J. Potential of fungal antagonists for biocontrol of Fusarium spp. In wheat and maize through competition in crop debris. Biocontrol Science and Technology 15 (2005) 229-242
31. Mach R.L., Peterbauer C.K., Payer K., Jaksits S., Woo S.L., Zeilinger S., Kullnig C.M., Lorito M., and Kubicek C.P. Expression of two major chitinase genes of Trichoderma atroviride (T. harzianum P1) is triggered by different regulatory signals. Applied Environmental Microbiology 65 (1999) 1858-1863
32. Mamarabadi M., Jensen D.F., and Lübeck M. Three different endochitinase-encoding genes (cr-ech58, cr-ech42 and cr-ech37) identified in the fungus Clonostachys rosea are differentially expressed. Current Genetics 54 (2008) 57-70
33. Marshall R.D. Glycoproteins. Annual Review of Biochemistry 41 (1972) 673-702
34. Møller K., Jensen B., Paludan Andersen H., Stryhn H., and Hockenhull J. Biocontrol of Pythium tracheiphilum in Chinese cabbage by Clonostachys rosea under field conditions. Biocontrol Science and Technology 13 (2003) 171-182
35. Pachenari A., and Dix N.J. Production of toxins and wall degrading enzymes of Gliocladium roseum. Transactions of the British Mycological Society 74 (1980) 561-566
36. Peterbauer C.K., Lorito M., Hayes C.K., Harman G.E., and Kubicek C.P. Molecular cloning and expression of the nag1 gene (N-acetyl-β-d-glucosaminidase-encoding gene) from Trichoderma harzianum P1. Current Genetics 304 (1996) 325-331
37. Ramot O., Viterbo A., Friesem D., Oppenheim A., and Chet I. Regulation of two homodimer hexosaminidases in the mycoparasitic fungus Trichoderma asperellum by glucosamine. Current Genetics 45 (2004) 205-213
38. Roberti R., Zakrisson E., Flamigni F., De Vero L., and Cesari A. Antagonistic fungi producing hydrolytic enzymes, active in degrading the cell wall of some foot rot pathogens (Fusarium spp.) of wheat. Journal of Plant Diseases and Protection 109 (2002) 101-108
39. Rose T.M., Schultz E.R., Henikoff J.G., Pietrokovski S., McCallum C.M., and Henikoff S. Consensus-degenerate hybrid oligonucleotide primers for amplification of distantly-related sequences. Nucleic Acids Research 26 (1998) 1628-1635
40. Rozen S., and Skaletsky H.J. Primer3 on the WWW for general users and for biologist programmers. In: Krawetz S., and Misener S. (Eds). Bioinformatics Methods and Protocols: Methods in Molecular Biology (2000), Humana Press, Totowa, NJ 365-386
41. Schroers H.J., Samuels G.J., Seifert K.A., and Gams W. Classification of the mycoparasite Gliocladium roseum in Clonostachys as C. rosea, its relationship to Bionectria ochroleuca, and notes on other Gliocladium-like fungi. Mycologia 91 (1999) 365-385
42. Seidl V., Huemer B., Seiboth B., and Kubicek C.P. A complete survey of Trichoderma chitinases reveals three distinct subgroups of family 18 chitinases. FEBS Journal 272 (2005) 5923-5939
43. Seidl V., Druzhinina I.S., and Kubicek C.P. A screening system for carbon sources enhancing beta-N-acetylglucosaminidase formation in Hypocrea atroviridis (Trichoderma atroviride). Microbiology 152 (2006) 2003-2012
44. Sutton J.C., Li D.W., Peng G., Yu H., Zhang P., and Valdebeneito-Sanhueza R.M. Gliocladium roseum: A versatile adversary of Botrytis cinerea in crops. Plant Disease 81 (1997) 316-328
45. Toledo A.V., Virla E., Humber R.A., Paradell S.L., and Lastra C.C. First record of Clonostachys rosea (Ascomycota: Hypocreales) as an entomopathogenic fungus of Oncometopia tucumana and Sonesimia grossa (Hemiptera: Cicadellidae) in Argentina. Journal of Invertebrate Pathology 92 (2006) 7-10
46. Tronsmo A., and Harman G.E. Detection and quantification of N-acetyl-β-d- glucosaminidase, chitobiosidase and endochitinase in solutions and on gels. Analytical Biochemistry 208 (1993) 74-79
47. Ulhoa C.J., and Peberdy J.F. Effect of carbon sources on chitobiase production by Trichoderma harzianum. Mycological Research 97 (1993) 45-48
48. Zeilinger S., Galhaup C., Payer K., Woo S.L., Mach R.L., Fekete C., Lorito M., and Kubicek C.P. Chitinase gene expression during mycoparasitic interaction of Trichoderma harzianum with its host. Fungal Genetics and Biology 26 (1999) 131-140