GUS and GFP transformation of the biocontrol strain Clonostachys rosea IK726 and the use of these marker genes in ecological studies

Mycological Research

Volumn 106, Issue 7, 2002, Pages 815-826

  Lübeck, Mette ^a   Knudsen, Inge M B ^a   Jensen, Birgit ^a   Thrane, Ulf ^b   Janvier, Céline ^c   Funck Jensen, Dan ^a  

^a UNIVERSITY OF COPENHAGEN   (Denmark)

^b TECHNICAL UNIVERSITY OF DENMARK   (Denmark)

^c CEMAGREF   (France)

Author keywords

[No Author keywords available]

Indexed keywords

BIOLOGICAL CONTROL; FUNGUS; GENETIC MARKER;

BIONECTRIA OCHROLEUCA; CLONOSTACHYS; CLONOSTACHYS ROSEA; DAUCUS CAROTA; FUNGI; FUSARIUM; FUSARIUM CULMORUM; HORDEUM VULGARE SUBSP. VULGARE; MYXOGASTRIA;

EID: 0036661060     PISSN: 09537562     EISSN: None     Source Type: Journal    
DOI: 10.1017/S095375620200607X     Document Type: Article

References (63)

1. Anon. (1999) COWTool. Version 1.1. Department of Biotechnology, Technical University of Denmark, Lyngby. Available at http://www.ibt.dtu.dk/mycology/cow/cow.htm.
2. Bae, Y.-S. & Knudsen, G. R. (2000) Cotransformation of Trichoderma harzianum with β-glucuronidase and green fluorescent protein genes provides a useful tool for monitoring fungal growth and activity in natural soils. Applied and Environmental Microbiology 66: 810-815.
3. Bulat, S. A., Lübeck, M., Mironenko, N., Jensen, D. F. & Lübeck, P. S. (1998) UP-PCR analysis and ITS1 ribotyping of strains of Trichoderma and Gliocladium. Mycological Research 102: 933-943.
4. Chalfie, M., Tu, Y., Euskirchen, G., Ward, W. W. & Prasher, D. C. (1994) Green fluorescent protein as a marker for gene expression. Science 263: 802-805.
5. Chiu, W.-I., Niwa, Y., Zeng, W., Hirano, T., Kobayashi, H. & Sheen, J. (1996) Engineered GFP as a vital reporter in plants. Current Biology 6: 325-330.
6. Couteaudier, Y., Daboussi, M. J., Eparvier, A., Langin, T. & Orcival, J. (1993) The GUS gene fusion system (Eschericia coli β-D-glucuronidase gene), a useful tool in studies of root colonization by Fusarium oxysporum. Applied and Environmental Microbiology 59: 1767-1773.
7. Crameri, A., Whitehorn, E. A., Tate, E. & Stemmer, W. P. C. (1996) Improved green fluorescent protein by molecular evolution using DNA shuffling. Nature Biotechnology 14: 315-319.
8. Cubitt, A. B., Heim, R., Adams, S. R., Boyd, A. E., Gross, L. A. & Tsien, R. Y. (1995) Understanding, improving and using green fluorescent proteins. Trends in Biochemical Sciences 20: 448-455.
9. Domsch, K. H., Gams, W. & Anderson, T. H. (1980) Compendium of Soil Fungi. Vol. 1. Academic Press, London.
10. Elad, Y., Chet, I. & Henis, Y. (1981) A selective medium for improving quantitative isolation of Trichoderma spp. from soil. Phytoparasitica 9: 59-67.
11. Elad, Y. & Kirshner, B. (1993) Survival in the phylloplane of an introduced biocontrol agent (Trichoderma harzianum) and populations of the plant pathogen Botrytis cinerea as modified by abiotic conditions. Phytoparasitica 21: 303-313.
12. Errampalli, D., Leung, K., Cassidy, M. B., Kostrzynska, M., Blears, M., Lee, H. & Trevors, J. T. (1999) Applications of the green fluorescent protein as a molecular marker in environmental microorganisms. Journal of Microbiological Methods 35: 187-199.
13. Fernández-Ábalos, J. M., Fox, H., Pitt, C., Wells, B. & Doonan, J. H. (1998) Plant-adapted green fluorescent protein is a versatile vital reporter for gene expression, protein localization and mitosis in the filamentous fungus, Aspergillus nidulans. Molecular Microbiology 27: 121-130.
14. Frisvad, J. C. & Thrane, U. (1987) Standardized high-performance liquid chromatography of 182 mycotoxins and other fungal metabolites based on alkylphenone retention indices and UV-VIS spectra (diode array detection). Journal of Chromatography 404: 195-214.
15. Green, H. & Jensen, D. F. (1995) A tool for monitoring Trichoderma harzianum: II. The use of a GUS transformant for ecological studies in the rhizosphere. Phytopathology 85: 1436-1440.
16. Green, H., Larsen, J., Olsson, P. A., Jensen, D. F. & Jakobsen, I. (1999) Suppression of the biocontrol agent Trichoderma harzianum by mycelium of the arbuscular mycorrhizal fungus Glomus intraradices in root-free soil. Applied and Environmental Microbiology 65: 1428-1434.
17. Green, H., Heiberg, N., Lejbølle, K. & Jensen, D. F. (2001) The use of a GUS transformant of Trichoderma harzianum, strain T3a, to study metabolic activity in the spermosphere and rhizosphere related to biocontrol of Pythium damping-off and root rot. European Journal of Plant Pathology 107: 349-359.
18. Heim, R., Cubitt, A. B. & Tsien, R. Y. (1994) Improved green fluorescence. Nature 373: 663-664.
19. Haran, S., Schickler, H. & Chet, I. (1996) Molecular mechanisms of lytic enzymes involved in the biocontrol activity of Trichoderma harzianum. Microbiology 142: 2321-2331.
20. Hjeljord, L. & Tronsmo, A. (1998) Trichoderma and Gliocladium in biological control: an overview. In Trichoderma & Gliocladium, Volume 2. Enzymes, biological control and commercial applications (G. E. Harman & C. P. Kubicek, eds): 131-151. Taylor & Francis Ltd., London, UK.
21. Jansson, J. K. & Prosser, J. I. (1997) Quantification of the presence and activity of specific microorganisms in nature. Molecular Biotechnology 7: 103-120.
22. Jefferson, R. A. (1987) Assaying chimaeric genes in plants: the GUS gene fusion system. Plant Molecular Biology Reporter 5: 387-405.
23. Jensen, B., Knudsen, I. M. B., Jensen, D. F. & Hockenhull, J. (2000) Biological seed treatment of cereals with fresh and long term stored formulations of Gliocladium roseum: Biocontrol efficacy against Fusarium culmorum. European Journal of Plant Pathology 106: 233-242.
24. Jørgensen, H. J. L., de Neergaard, E. & Smedegaard-Petersen, V. (1993) Histological examination of the interaction between Rhynchosporium secalis and susceptible and resistant cultivars of barley. Physiological and Molecular Plant Pathology 42: 345-358.
25. Jørgensen, H. J. L., Andresen, H. & Smedegaard-Petersen, V. (1996) Control of Drechslera teres and other barley pathogens by preinoculation with Bipolaris sorokiniana and Septoria nodorum. Phytopathology 86: 602-607.
26. Keinath, A. P., Fravel, D. R. & Papavizas, G. C. (1991) Potential of Gliocladium roseum for biocontrol of Verticillium dahlia. Phytopathology 81: 644-648.
27. Knudsen, I. M. B., Hockenhull, J. & Jensen, D. F. (1995) Biocontrol of seedling diseases caused by Fusarium culmorum and Bipolaris sorokiniana: effects of selected fungal antagonists on growth and yield components. Plant Pathology 44: 467-477.
28. Knudsen, I. M. B., Jensen, B., Jensen, D. F. & Hockenhull, J. (1996) Occurrence of Gliocladium roseum on barley roots in sand and field soil. In Monitoring Antagonistic Fungi Deliberately Released into the Environment (D. F. Jensen, A. Tronsmo & H.-B. Jansson, eds): 33-37. [Developments in Plant Pathology, vol. 8.] Kluwer Academic Publishers, Dordrecht.
29. Knudsen, I. M. B., Hockenhull, J., Jensen, D. F., Gerhardson, B., Hökeberg, M., Tahvonen, R., Teperi, E., Sundheim, L. & Henriksen, B. (1997) Selection of biological control agents for controlling soil- and seed-borne pathogens in the field. European Journal of Plant Pathology 103: 775-784.
30. Laing, S. A. K. & Deacon, J. W. (1991) Video microscopical comparison of mycoparasitism by Pythium oligandrum, P. nunn and an unnamed Pythium species. Mycological Research 95: 469-479.
31. Liljeroth, E., Jansson, H.-B. & Schäfer, W. (1993) Transformation of Bipolaris sorokiniana with the GUS-gene and use for studying fungal colonization of barley roots. Phytopathology 84: 1484-1489.
32. Lo, C. T., Nelson, E. B., Hayes, C. K. & Harman, G. E. (1998) Ecological studies of transformed Trichoderma harzianum strain 1295-22 in the rhizosphere and on the phylloplane of creeping bentgrass. Phytopathology 88: 129-136.
33. Madsen, A. M. & de Neergaard, E. (1999) Interactions between the mycoparasite Pythium oligandrum and sclerotia of the plant pathogen Sclerotinia sclerotiorum. European Journal of Plant Pathology 105: 761-768.
34. Maor, R., Puyesky, M., Horwitz, B. A. & Sharon, A. (1998) Use of green fluorescent protein (GFP) for studying development and fungal-plant interaction in Cochliobolus heterostrophus. Mycological Research 102: 491-496.
35. Mikkelsen, L., Roulund, N., Lübeck, M. & Jensen, D. F. (2000) The perennial ryegrass endophyte Neotyphodium lolii genetically transformed with the green fluorescent protein gene (GFP) and visualization in the host plant. Mycological Research 105: 644-650.
36. Murray, F. R., Latch, G. C. M. & Scott, D. B. (1992) Surrogate transformation of perennial ryegrass, Lolium perenne, using genetically modified Acremonium endophyte. Molecular and General Genetics 233: 1-9.
37. Nielsen, N.-P. V., Carstensen, J. M. & Smedsgaard, J. (1998) Aligning of single and multiple wavelength chromatographic profiles for chemometric data analysis using correlation optimised warping. Journal of Chromatography 805: 17-35.
38. Nielsen, N.-P. V., Smedsgaard, J. & Frisvad, J. C. (1999) Full second-order chromatographic/spectrometric data matrices for automated sample identification and component analysis by non data reducing image analysis. Analytical Chemistry 71: 727-735.
39. Pachenari, A. & Dix, N. J. (1980) Production of toxins and wall degrading enzymes of Gliocladium roseum. Transactions of the British Mycological Society 74: 561-566.
40. Punt, P. J., Oliver, R. P., Dingemanse, M. A. & Pouwels, P. H. (1987) Transformation of Aspergillus based on the hygromycin B resistance marker from Escherichia coli. Gene 56: 117-124.
41. Roberts, I. N., Oliver, R. P., Dingemanse, M. A., Pouwels, P. H. & van den Hondel, C. A. M. J. (1989) Expression of the Escherichia coli β-glucuronidase gene in industrial and phytopathogenic filamentous fungi. Current Genetics 15: 177-180.
42. Rohel, E. A., Payne, A. C., Fraaije, B. A. & Hollomon, D. W. (2001) Exploring infection of wheat and carbohydrate metabolism in Mycosphaerella graminicola transformants with differentially regulated green fluorescent protein expression. Molecular Plant-Microbe Interactions 14: 156-163.
43. Sambrook, J., Fritsch, E. E. & Maniatis, T. (1989) Molecular Cloning: a laboratory manual. Cold Spring Harbor Laboratory Press, Cold Spring Harbor, NY.
44. Samson, R. A., Hoekstra, E. S., Frisvad, J. C. & Filtenborg, O. (1995) Introduction to Food-borne Fungi. Centraalbureau voor Schimmelcultures, Baarn.
45. Schirmböck, M., Lorito, M., Wang, Y.-L., Hayes, C. K., Arisan-Atac, I., Scala, F., Harman, G. E. & Kubicek, C. P. (1994) Parallel formation and synergism of hydrolytic enzymes and peptaibol antibiotics, molecular mechanisms involved in the antagonistic action of Trichoderma harzianum against phytopathogenic fungi. Applied and Environmental Microbiology 60: 4364-4370.
46. Schroers, H.-J. (2001) A monograph of Bionectria (Ascomycota, Hypocreales, Bionectriaceae) and its Clonostachys anamorphs. Studies in Mycology 46: 1-214.
47. Schroers, H.-J., Samuels, G. J., Seifert, K. A. & Gams, W. (1999) Classification of the mycoparasite Gliocladium roseum in Clonostachys as C. rosea, its relationship to Bionectria ochroleuca, and notes on other Gliocladium-like fungi. Mycologia 91: 365-385.
48. Seh, M. L. & Kenerley, C. M. (1988) Protoplast isolation and regeneration and nuclear staining of mycoparasitic Gliocladium species. Journal of Microbiological Methods 8: 121-130.
49. Smedsgaard, J. (1997) Micro-scale extraction procedure for standardized screening of fungal metabolite production in cultures. Journal of Chromatography 760: 264-270.
50. Spellig, T., Bottin, A. & Kahmann, R. (1996) Green fluorescent protein (GFP) as a new vital marker in the phytopathogenic fungus Ustilago maydis. Molecular and General Genetics 252: 503-509.
51. Stewart, A. & Harrison, Y. A. (1989) Mycoparasitism of sclerotia of Sclerotium cepivorum. Australas. Plant Pathology 18: 10-14.
52. Stuurman, N., Pacios Bras, C., Schlaman, H. R. M., Wijfjes, A. H. M., Bloemberg, G. & Spaink, H. P. (2000) Use of green fluorescent protein color variants expressed on stable broad-host-range vectors to visualize Rhizobia interacting with plants. Molecular Plant-Microbe Interactions 13: 1163-1169.
53. Sutton, J. C., Li, D.-W., Peng, G., Yu, H., Zhang, P. & Valdebeneito-Sanhueza, R. M. (1997) Gliocladium roseum: a versatile adversary of Botrytis cinerea in crops. Plant Disease 81: 316-328.
54. Thomas, M. D. & Kenerley, C. M. (1989) Transformation of the mycoparasite Gliocladium. Current Genetics 15: 415-420.
55. Thrane, C., Lübeck, M., Green, H., Degefu, Y., Thrane, U., Allerup, S. & Jensen, D. F. (1995) A tool for monitoring Trichoderma harzianum: I. Transformation of Trichoderma harzianum by protoplast technology. Phytopathology 85: 1428-1435.
56. Tombolini, R., Unge, A., Davey, M. E., de Bruijn, F. J. & Jansson, J. K. (1997) Flow cytometric and microscopic analysis of GFP-tagged Pseudomonas fluorescens bacteria. FEMS Microbiology and Ecology 22: 17-28.
57. Tombolini, R., van der Gaag, D. J., Gerhardson, B. & Jansson, J. K. (1999) Colonization pattern of the biocontrol strain Pseudomonas chlororaphis MA 342 on barley seeds visualized by using green fluorescent protein. Applied and Environmental Microbiology 65: 3674-3680.
58. Tsien, R. Y. (1998) The green fluorescent protein. Annual Review of Biochemistry 67: 509-544.
59. Turhan, G. (1993) Mycoparasitism of Alternaria alternata by an additional eight fungi indicating the existence of further unknown candidates for biological control. Journal of Phytopathology 138: 283-292.
60. Vakili, N. G. (1992) Biological seed treatment of corn with mycopathogenic fungi. Journal of Phytopathology 134: 313-323.
61. Whipps, J. M. (1987) Behaviour of fungi antagonistic to Sclerotinia sclerotiorum on plant tissue segments. Journal of General Microbiology 133: 1495-1501.
62. Yates, I. E., Hiett, K. L., Kapczynski, D. R., Smart, W., Glenn, A. E., Hinton, D. M., Bacon, C. W., Meinersmann, R., Liu, S. & Jaworski, A. J. (1999) GUS transformation of the maize fungal endophyte Fusarium moniliforme. Mycological Research 103: 129-136.
63. Zhou, M., Upson, R. H., Diwu, Z. & Haugland, R. P. (1996) A fluorogenic substrate for β-glucuronidase: applications in fluorometric, polyacrylamide gel and histochemical assays. Journal of Biochemical and Biophysical Methods 33: 197-205.