Synaptotagmin C2B domain regulates Ca2+-triggered fusion in vitro: Critical residues revealed by scanning alanine mutagenesis

Journal of Biological Chemistry

Volumn 283, Issue 46, 2008, Pages 31763-31775

  Gaffaney, Jon D ^a   Dunning, F Mark ^a   Wang, Zhao ^a   Hui, Enfu ^a   Chapman, Edwin R ^a,b  

^a UNIVERSITY OF WISCONSIN MADISON   (United States)

^b 129 SMI ^*  (United States)

Author keywords

[No Author keywords available]

Indexed keywords

MUTAGENESIS; PLANTS (BOTANY); SCANNING;

BINDING LOOPS; BINDING MOTIFS; C2 DOMAINS; CRITICAL RESIDUES; CYTOSOLIC DOMAINS; DIFFERENT EFFECTS; DO-MAINS; EQUIMOLAR CONCENTRATIONS; IN VITRO; PHOSPHATIDYL SERINES; PHOSPHATIDYLETHANOL AMINES; SNAP-25; STRUCTURAL DETERMINANTS; SYNAPTIC VESICLES; SYNAPTOTAGMIN; SYNTAXIN; VESICLE AGGREGATIONS;

CALCIUM;

ALANINE; ARGININE; CALCIUM; HYBRID PROTEIN; PHOSPHATIDYLETHANOLAMINE; PHOSPHATIDYLSERINE; SNARE PROTEIN; SYNAPTOSOMAL ASSOCIATED PROTEIN 25; SYNAPTOTAGMIN; SYNAPTOTAGMIN I; SYNTAXIN;

AMINO ACID SUBSTITUTION; ARTICLE; ASSAY; CYTOSOL; IN VITRO STUDY; MEMBRANE; MEMBRANE BINDING; MOUSE; MUTAGENESIS; MUTATION; NONHUMAN; PRIORITY JOURNAL; PROTEIN DOMAIN; REGULATORY MECHANISM;

ALANINE; ANIMALS; CALCIUM; CELL MEMBRANE; CYTOSOL; LIPOSOMES; MICE; MODELS, MOLECULAR; MUTAGENESIS; PROTEIN BINDING; PROTEIN STRUCTURE, QUATERNARY; PROTEIN STRUCTURE, TERTIARY; QA-SNARE PROTEINS; SYNAPTOTAGMINS;

EID: 57649136398     PISSN: 00219258     EISSN: 1083351X     Source Type: Journal    
DOI: 10.1074/jbc.M803355200     Document Type: Article

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