Reconstitution of Ca2+-Regulated Membrane Fusion by Synaptotagmin and SNAREs

Science

Volumn 304, Issue 5669, 2004, Pages 435-438

  Tucker, Ward C ^a   Weber, Thomas ^b   Chapman, Edwin R ^a  

^a UNIVERSITY OF WISCONSIN MADISON   (United States)

^b MOUNT SINAI SCHOOL OF MEDICINE   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

CELL MEMBRANES; CYTOLOGY; LIPIDS; POSITIVE IONS; PROTEINS;

MEMBRANE FUSION; SYNAPTOVEBRIN; SYNTAXIN;

CALCIUM;

BOTULINUM TOXIN A; CALCIUM ION; SNARE PROTEIN; SYNAPTOBREVIN; SYNAPTOSOMAL ASSOCIATED PROTEIN 25; SYNAPTOTAGMIN; SYNTAXIN; CALCIUM; CALCIUM BINDING PROTEIN; LIPID; LIPOSOME; MEMBRANE PROTEIN; NERVE PROTEIN; SNAP25 PROTEIN, MOUSE; SNAP25 PROTEIN, RAT; SYNAPTOTAGMIN I; SYT1 PROTEIN, MOUSE; SYT1 PROTEIN, RAT;

BIOLOGY;

ARTICLE; CALCIUM BINDING; CALCIUM TRANSPORT; EXOCYTOSIS; LIPID COMPOSITION; MEMBRANE FUSION; PRIORITY JOURNAL; PROTEIN DEGRADATION; PROTEIN DOMAIN; SYNAPSE VESICLE; ANIMAL; BINDING SITE; CHEMISTRY; FLUORESCENCE RESONANCE ENERGY TRANSFER; LIPID BILAYER; METABOLISM; MOUSE; MUTATION; PROTEIN TERTIARY STRUCTURE; RAT;

ANIMALS; BINDING SITES; CALCIUM; CALCIUM-BINDING PROTEINS; EXOCYTOSIS; FLUORESCENCE RESONANCE ENERGY TRANSFER; LIPID BILAYERS; LIPIDS; LIPOSOMES; MEMBRANE FUSION; MEMBRANE GLYCOPROTEINS; MEMBRANE PROTEINS; MICE; MUTATION; NERVE TISSUE PROTEINS; PROTEIN STRUCTURE, TERTIARY; QA-SNARE PROTEINS; R-SNARE PROTEINS; RATS; SYNAPTIC VESICLES; SYNAPTOSOMAL-ASSOCIATED PROTEIN 25; SYNAPTOTAGMIN I; SYNAPTOTAGMINS;

EID: 1942520207     PISSN: 00368075     EISSN: None     Source Type: Journal    
DOI: 10.1126/science.1097196     Document Type: Article

References (25)

1. T. Weber et al., Cell 92, 759 (1998).
2. T. Sollner et al., Nature 362, 318 (1993).
3. J. A. McNew et al., Nature 407, 153 (2000).
4. F. Parlati et al., Proc. Natl. Acad. Sci. U.S.A. 96, 12565 (1999).
5. B. Katz, The Release of Neural Transmitter Substances (Charles C. Thomas, Springfield, IL, 1969).
6. G. J. Augustine, Curr. Opin. Neurobiol. 11, 320 (2001).
7. N. Brose, A. G. Petrenko, T. C. Sudhof, R. Jahn, Science 256, 1021 (1992).
8. W. C. Tucker, E. R. Chapman, Biochem. J. 366, 1 (2002).
9. D. K. Struck, D. Hoekstra, R. E. Pagano, Biochemistry 20, 4093 (1981).
10. Detailed materials and methods are available as supporting material on Science Online.
11. R. Jahn, T. C. Sudhof, Annu. Rev. Neurosci. 17, 219 (1994).
12. C. Walch-Solimena et al., J. Cell Blol. 128, 637 (1995).
13. G. Schiavo, M. Matteoli, C. Montecucco, Physiol. Rev. 80, 717 (2000).
14. X. Zhang, J. Rizo, T. C. Sudhot, Biochemistry 37, 12395 (1998).
15. C. A. Earles, J. Bai, P. Wang, E. R. Chapman, J. Cell Biol. 154, 1117 (2001).
16. I. Fernandez et al., Neuron 32, 1057 (2001).
17. E. R. Chapman, S. An, J. M. Edwardson, R. Jahn, J. Biol. Chem. 271, 5844 (1996).
18. A. F. Davis et al., Neuron 24, 363 (1999).
19. H. Lundh, S. Leander, S. Thesleff, J. Neurol. Sci. 32, 29 (1977).
20. S. G. Cull-Candy, H. Lundh, S. Thesleff, J. Physiol. 260, 177 (1976).
21. L. K. Mahal, S. M. Sequeira, J. M. Gureasko, T. H. Sollner, J. Cell Biol. 158, 273 (2002).
22. M. Craxton, M. Goedert, FEBS Lett. 460, 417 (1999).
23. C. Bagala et al., Biochem. Biophys. Res. Commun. 310, 1041 (2003).
24. M. Fukuda, K. Mikoshiba, J. Biol. Chem. 274, 31428 (1999).
25. We thank M. Dong for technical assistance with the BoNT/B experiments and A. Riccio for assistance with preparation of t-SNARE heterodimers. We also thank M. Jackson, M. Edwardson, J. Weisshaar, and all members of the Chapman lab for comments and discussions. This study was supported by grants from the NIH (NIGMS GM 56827 and NIMH MH 61876 to E.R.C.; NIGMS GM 66313 to T.W.), AHA0440168N (E.R.C.), and the Milwaukee Foundation (E.R.C.). E.R.C. is a Pew Scholar in the Biomedical Sciences. W.C.T. is supported by a Postdoctoral Individual NSRA from the NIH.