Localization and interaction of the proteins constituting the GAL genetic switch in Saccharomyces cerevisiae

Eukaryotic Cell

Volumn 7, Issue 12, 2008, Pages 2061-2068

  Wightman, Raymond ^a   Bell, Rachel ^a   Reece, Richard J ^a  

^a UNIVERSITY OF MANCHESTER   (United Kingdom)

Author keywords

[No Author keywords available]

Indexed keywords

GAL3 PROTEIN, S CEREVISIAE; GAL4 PROTEIN, S CEREVISIAE; GAL80 PROTEIN, S CEREVISIAE; GALACTOSE; REPRESSOR PROTEIN; SACCHAROMYCES CEREVISIAE PROTEIN; TRANSCRIPTION FACTOR;

ARTICLE; CELL NUCLEUS; CYTOLOGY; CYTOPLASM; GENETICS; METABOLISM; PROTEIN BINDING; PROTEIN TRANSPORT; SACCHAROMYCES CEREVISIAE;

CELL NUCLEUS; CYTOPLASM; GALACTOSE; PROTEIN BINDING; PROTEIN TRANSPORT; REPRESSOR PROTEINS; SACCHAROMYCES CEREVISIAE; SACCHAROMYCES CEREVISIAE PROTEINS; TRANSCRIPTION FACTORS;

EUKARYOTA; SACCHAROMYCES CEREVISIAE;

EID: 57349155872     PISSN: 15359778     EISSN: None     Source Type: Journal    
DOI: 10.1128/EC.00261-08     Document Type: Article

References (35)

1. Anders, A., H. Lilie, K. Franke, L. Kapp, J. Stelling, E. D. Gilles, and K. D. Breunig. 2006. The galactose switch in Kluyveromyces lactis depends on nuclear competition between Gal4 and Gal1 for Gal80 binding. J. Biol. Chem. 281:29337-29348.
2. Barberis, A., J. Pearlberg, N. Simkovich, S. Farrell, P. Reinagel, C. Bamdad, G. Sigal, and M. Ptashne. 1995. Contact with a component of the polymerase II holoenzyme suffices for gene activation. Cell 81:359-368.
3. Bhat, P. J., and J. E. Hopper. 1992. Overproduction of the GAL1 or GAL3 protein causes galactose-independent activation of the GAL4 protein: evidence for a new model of induction for the yeast GAL/MEL regulon. Mol. Cell. Biol. 12:2701-2707.
4. Bhaumik, S. R., T. Raha, D. P. Aiello, and M. R. Green. 2004. In vivo target of a transcriptional activator revealed by fluorescence resonance energy transfer. Genes Dev. 18:333-343.
5. Broach, J. R. 1979. Galactose regulation in Saccharomyces cerevisiae. The enzymes encoded by the GAL7, 10, 1 cluster are co-ordinately controlled and separately translated. J. Mol. Biol. 131:41-53.
6. Davis, T. N., and E. G. D. Muller. 2007. Measuring the proximity of proteins in living cells by fluorescence resonance energy transfer between CFP and YFP. Methods Microbiol. 36:269-280.
7. Feige, J. N., D. Sage, W. Wahli, B. Desvergne, and L. Gelman. 2005. PixFRET, an ImageJ plug-in for FRET calculation that can accommodate variations in spectral bleed-throughs. Microsc. Res. Tech. 68:51-58.
8. Ghaemmaghami, S., W. K. Huh, K. Bower, R. W. Howson, A. Belle, N. Dephoure, E. K. O'Shea, and J. S. Weissman. 2003. Global analysis of protein expression in yeast. Nature 425:737-741.
9. Giaever, G., A. M. Chu, L. Ni, C. Connelly, L. Riles, S. Veronneau, S. Dow, A. Lucau-Danila, K. Anderson, B. Andre, A. P. Arkin, A. Astromoff, M. El-Bakkoury, R. Bangham, R. Benito, S. Brachat, S. Campanaro, M. Curtiss, K. Davis, A. Deutschbauer, K. D. Entian, P. Flaherty, F. Foury, D. J. Garfinkel, M. Gerstein, D. Gotte, U. Guldener, J. H. Hegemann, S. Hempel, Z. Herman, D. F. Jaramillo, D. E. Kelly, S. L. Kelly, P. Kotter, D. LaBonte, D. C. Lamb, N. Lan, H. Liang, H. Liao, L. Liu, C. Luo, M. Lussier, R. Mao, P. Menard, S. L. Ooi, J. L. Revuelta, C. J. Roberts, M. Rose, P. Ross-Macdonald, B. Scherens, G. Schimmack, B. Shafer, D. D. Shoemaker, S. Sookhai-Mahadeo, R. K. Storms, J. N. Strathern, G. Valle, M. Voet, G. Volckaert, C. Y. Wang, T. R. Ward, J. Wilhelmy, E. A. Winzeler, Y. Yang, G. Yen, E. Youngman, K. Yu, H. Bussey, J. D. Boeke, M. Snyder, P. Philippsen, R. W. Davis, and M. Johnston. 2002. Functional profiling of the Saccharomyces cerevisiae genome. Nature 418:387-391.
10. Gietz, R. D., and A. Sugino. 1988. New yeast-Escherichia coli shuttle vectors constructed with in vitro mutagenized yeast genes lacking six-base pair restriction sites. Gene 74:527-534.
11. Griggs, D. W., and M. Johnston. 1991. Regulated expression of the GAL4 activator gene in yeast provides a sensitive genetic switch for glucose repression. Proc. Natl. Acad. Sci. USA 88:8597-8601.
12. Holden, H. M., I. Rayment, and J. B. Thoden. 2003. Structure and function of enzymes of the Leloir pathway for galactose metabolism. J. Biol. Chem. 278:43885-43888.
13. Huh, W. K., J. V. Falvo, L. C. Gerke, A. S. Carroll, R. W. Howson, J. S. Weissman, and E. K. O'Shea. 2003. Global analysis of protein localization in budding yeast. Nature 425:686-691.
14. Johnston, M. 1987. A model fungal gene regulatory mechanism: the GAL genes of Saccharomyces cerevisiae. Microbiol. Rev. 51:458-476.
15. Leuther, K. K., and S. A. Johnston. 1992. Nondissociation of GAL4 and GAL80 in vivo after galactose induction. Science 256:1333- 1335.
16. Lohr, D., P. Venkov, and J. Zlantanova. 1995. Transcriptional regulation in the yeast GAL gene family: a complex genetic network. FASEB J. 9:777-787.
17. Nguyen, A. W., and P. S. Daugherty. 2005. Evolutionary optimization of fluorescent proteins for intracellular FRET. Nat. Biotechnol. 23:355-360.
18. Overton, M. C., and K. J. Blumer. 2000. G-protein-coupled receptors function as oligomers in vivo. Curr. Biol. 10:341-344.
19. Parthun, M. R., and J. A. Jaehning. 1992. A transcriptionally active form of GAL4 is phosphorylated and associated with GAL80. Mol. Cell. Biol. 12:4981-4987.
20. Peng, G., and J. E. Hopper. 2000. Evidence for Gal3p's cytoplasmic location and Gal80p's dual cytoplasmic-nuclear location implicates new mechanisms for controlling Gal4p activity in Saccharomyces cerevisiae. Mol. Cell. Biol. 20:5140-5148.
21. Peng, G., and J. E. Hopper. 2002. Gene activation by interaction of an inhibitor with a cytoplasmic signaling protein. Proc. Natl. Acad. Sci. USA 99:8548-8553.
22. Platt, A., and R. J. Reece. 1998. The yeast galactose genetic switch is mediated by the formation of a Gal4p/Gal80p/Gal3p complex. EMBO J. 17:4086-4091.
23. Reece, R. J. 2000. Molecular basis of nutrient controlled gene expression in Saccharomyces cerevisiae. Cell. Mol. Life Sci. 57:1161-1171.
24. Rubio-Texeira, M. 2005. A comparative analysis of the GAL genetic switch between not-so-distant cousins: Saccharomyces cerevisiae versus Kluyveromyces lactis. FEMS Yeast Res. 5:1115-1128.
25. Sellick, C. A., and R. J. Reece. 2005. Eukaryotic transcription factors as direct nutrient sensors. Trends Biochem. Sci. 30:405-412.
26. Sheff, M. A., and K. S. Thorn. 2004. Optimized cassettes for fluorescent protein tagging in Saccharomyces cerevisiae. Yeast 21:661-670.
27. Sherman, F. 1991. Getting started with yeast. Methods Enzymol. 194:3-21.
28. Sil, A. K., S. Alam, P. Xin, L. Ma, M. Morgan, C. M. Lebo, M. P. Woods, and J. E. Hopper. 1999. The Gal3p-Gal80p-Gal4p transcription switch of yeast: Gal3p destabilizes the Gal80p-Gal4p complex in response to galactose and ATP. Mol. Cell. Biol. 19:7828-7840.
29. Singh, A., S. Severance, N. Kaur, W. Wiltsie, and D. J. Kosman. 2006. Assembly, activation, and trafficking of the Fet3p.Ftr1p high affinity iron permease complex in Saccharomyces cerevisiae. J. Biol. Chem. 281:13355-13364.
30. Stansfield, I., K. M. Jones, V. V. Kushnirov, A. R. Dagkesamanskaya, A. I. Poznyakovski, S. V. Paushkin, C. R. Nierras, B. S. Cox, M. D. Ter-Avanesyan, and M. F. Tuite. 1995. The products of the SUP45 (eRF1) and SUP35 genes interact to mediate translation termination in Saccharomyces cerevisiae. EMBO J. 14:4365-4373.
31. Suzuki-Fujimoto, T., M. Fukuma, K.-I. Yano, H. Sakurai, A. Vonika, S. A. Johnston, and T. Fukasawa. 1996. Analysis of the galactose signal transduction pathway in Saccharomyces cerevisiae: Interaction between Gal3p and Gal80p. Mol. Cell. Biol. 16:2504-2508.
32. Timson, D. J., and R. J. Reece. 2002. Kinetic analysis of yeast galactokinase: implications for transcriptional activation of the GAL genes. Biochimie 84:265-272.
33. Warren, D. T., P. D. Andrews, C. W. Gourlay, and K. R. Ayscough. 2002. Sla1p couples the yeast endocytic machinery to proteins regulating actin dynamics. J. Cell Sci. 115:1703-1715.
34. Yarger, J. G., H. O. Halvorson, and J. E. Hopper. 1984. Regulation of galactokinase (GAL1) enzyme accumulation in Saccharomyces cerevisiae. Mol. Cell. Biochem. 61:173-182.
35. Zenke, F. T., R. Engles, V. Vollenbroich, J. Meyer, C. P. Hollenberg, and K. D. Breunig. 1996. Activation of Gal4p by galactose-dependent interaction of galactokinase and Gal80p. Science 272:1662-1665.