Kinetic analysis of yeast galactokinase: Implications for transcriptional activation of the GAL genes

Biochimie

Volumn 84, Issue 4, 2002, Pages 265-272

  Timson, David J ^a   Reece, Richard J ^a  

^a UNIVERSITY OF MANCHESTER   (United Kingdom)

Author keywords

Enzyme mechanism; Gal3p; Gal80p; Galactosemia; Gene expression

Indexed keywords

ADENOSINE TRIPHOSPHATE; GALACTOKINASE; GALACTOSE; GALACTOSE 1 PHOSPHATE; GALACTOSE PHOSPHATE; UNCLASSIFIED DRUG; ENZYME INHIBITOR; GAL3 PROTEIN, S CEREVISIAE; ISOTOPE; LIGAND; SACCHAROMYCES CEREVISIAE PROTEIN; TRANSCRIPTION FACTOR;

ARTICLE; CATABOLISM; CATALYSIS; CHEMICAL REACTION KINETICS; ENZYME KINETICS; ENZYME SPECIFICITY; ENZYMOLOGY; GENE EXPRESSION REGULATION; GENE SWITCHING; NONHUMAN; PH MEASUREMENT; PROTEIN EXPRESSION; PROTEIN FUNCTION; TRANSCRIPTION INITIATION; TRANSCRIPTION TERMINATION; YEAST; BINDING COMPETITION; BIOSYNTHESIS; GENETICS; KINETICS; METABOLISM; PH; PROTEIN BINDING; SACCHAROMYCES CEREVISIAE; TRANSACTIVATION;

ADENOSINE TRIPHOSPHATE; BINDING, COMPETITIVE; ENZYME INHIBITORS; GALACTOKINASE; GALACTOSE; GALACTOSEPHOSPHATES; HYDROGEN-ION CONCENTRATION; ISOTOPES; KINETICS; LIGANDS; PROTEIN BINDING; SACCHAROMYCES CEREVISIAE; SACCHAROMYCES CEREVISIAE PROTEINS; SUBSTRATE SPECIFICITY; SUPPORT, NON-U.S. GOV'T; TRANS-ACTIVATION (GENETICS); TRANSCRIPTION FACTORS;

EID: 0035997015     PISSN: 03009084     EISSN: None     Source Type: Journal    
DOI: 10.1016/S0300-9084(02)01399-8     Document Type: Article

References (35)

1. Molecular basis of nutrient controlled gene expression in Saccharomyces cerevisiae
2. Convergent evolution of similar enzymatic function on different protein folds: The hexokinase, ribokinase, and galactokinase families of sugar kinases
3. Structure and mechanism of homoserine kinase: Prototype for the GHMP kinase superfamily
4. Structural basis for the catalysis and substrate specificity of homoserine kinase
5. The fundamental importance of human galactose metabolism: Lessons from genetics and biochemistry
6. Molecular basis of disorders of human galactose metabolism: Past, present, and future
7. Novel mutations in 13 probands with galactokinase deficiency
8. A founder mutation in the GK1 gene is responsible for galactokinase deficiency in Roma (Gypsies)
9. A genetic factor for age-related cataract: Identification and characterization of a novel galactokinase variant, 'Osaka', in Asians
10. Galactose-dependent reversible interaction of Gal3p with Gal80p in the induction pathway of Gal4p-activated genes of Saccharomyces cerevisiae
11. Novel Gal3 proteins showing altered Gal80p binding cause constitutive transcription of Gal4p-activated genes in Saccharomyces cerevisiae
12. Activation of Gal4p by galactose-dependent interaction of galactokinase and Gal80p
13. Gal3p and Gal1p interact with the transcriptional repressor Gal80p to form a complex of 1:1 stoichiometry
14. Analysis of the GAL3 signal transduction pathway activating GAL4 protein-dependent transcription in Saccharomyces cerevisiae
15. Non-pleiotropic nature of the gal3 mutation in yeast
16. The mechanism of inducer formation in gal3 mutants of the yeast galactose system is independent of normal galactose metabolism and mitochondrial respiratory function
17. The insertion of two amino acids into a transcriptional inducer converts it into a galactokinase
18. The yeast galactose genetic switch is mediated by the formation of a Gal4p/Gal80p/Gal3p complex
19. SLU7 and a novel activity, SSF1, act during the PRP16-dependent step of yeast pre-mRNA splicing
20. An algorithm for least squares estimation of nonlinear parameters
21. Buffers of constant ionic strength for studying pH-dependent processes
22. A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding
23. Cleavage of structural proteins during the assembly of the head of bacteriophage T4
24. Purification and properties of galactokinase from Saccharomyces cerevisiase
25. Overproduction of the GAL1 or GAL3 protein causes galactose-independent activation of the GAL4 protein: Evidence for a new model of induction for the yeast GAL/MEL regulation
26. Kinetic studies with liver galactokinase
27. Kinetic studies of Escherichia coli galactokinase
28. Galactokinase of Vicia faba seeds
29. The enzymes of the galactose operon in Escherichia coli. III. The size and composition of galactokinase
30. Mutations in the active site of Escherichia coli phosphofructokinase
31. Structure of Escherichia coli ribokinase in complex with ribose and dinucleotide determined to 1.8 Å resolution: Insights into a new family of kinase structures
32. Use of pH studies to elucidate the chemical mechanism of yeast hexokinase
33. Enzyme-catalyzed phosphoryl transfer reactions
34. The crystal structure of the bifunctional enzyme 6-phosphofructo-2-kinase/fructose-2,6-bisphosphatase reveals distinct domain homologies