Mice lacking the Na+/H+ exchanger 2 have impaired recovery of intestinal barrier function

American Journal of Physiology - Gastrointestinal and Liver Physiology

Volumn 295, Issue 4, 2008, Pages

  Moeser, Adam J ^a   Nighot, Prashant K ^a   Ryan, Kathleen A ^a   Simpson, Janet E ^b   Clarke, Lane L ^b   Blikslager, Anthony T ^a  

^a NORTH CAROLINA STATE UNIVERSITY   (United States)

^b UNIVERSITY OF MISSOURI   (United States)

Author keywords

Intestinal permeability; Ischemia; Na+ H + exchange; Tight junction

Indexed keywords

CLAUDIN 1; MANNITOL; OCCLUDIN; SERINE; SODIUM PROTON EXCHANGE PROTEIN 2;

ANIMAL TISSUE; ARTICLE; CONTROLLED STUDY; ELECTRIC RESISTANCE; IN VITRO STUDY; IN VIVO STUDY; INTESTINE ABSORPTION; INTESTINE ISCHEMIA; MEMBRANE PERMEABILITY; MESENTERY; MOUSE; NONHUMAN; PRIORITY JOURNAL; PROTEIN EXPRESSION; PROTEIN FUNCTION; PROTEIN PHOSPHORYLATION; WILD TYPE;

ANIMALS; INTESTINAL MUCOSA; INTESTINE, SMALL; ISCHEMIA; MANNITOL; MEMBRANE PROTEINS; MICE; MICE, KNOCKOUT; PERMEABILITY; PHOSPHORYLATION; SODIUM-HYDROGEN ANTIPORTER; TIGHT JUNCTIONS;

EID: 57349146249     PISSN: 01931857     EISSN: 15221547     Source Type: Journal    
DOI: 10.1152/ajpgi.00538.2007     Document Type: Article

References (33)

1. Argenzio RA, Lecce J, Powell DW. Prostanoids inhibit intestinal NaCl absorption in experimental porcine cryptosporidiosis. Gastroenterology 104: 440-447, 1993.
2. + exchanger isoform 2 is the predominant NHE isoform in murine colonic crypts and its lack causes NHE3 upregulation. Am J Physiol Gastrointest Liver Physiol 287: G125-G133, 2004.
3. Balda MS, Gonzalez-Mariscal L, Matter K, Cereijido M, Anderson JM. Assembly of the tight junction: the role of diacylglycerol. J Cell Biol 123: 293-302, 1993.
4. Blikslager AT, Roberts MC, Argenzio RA. Prostaglandin-induced recovery of barrier function in porcine ileum is triggered by chloride secretion. Am J Physiol Gastrointest Liver Physiol 276: G28-G36, 1999.
5. Blikslager AT, Roberts MC, Rhoads JM, Argenzio RA. Prostaglandins I2 and E2 have a synergistic role in rescuing epithelial barrier function in porcine ileum. J Clin Invest 100: 1928-1933, 1997.
6. + exchangers NHE1-3: plasma membrane NHE2 has a rapid rate of degradation. Am J Physiol Cell Physiol 281: C2039-C2048, 2001.
7. + exchangers NHE2 and NHE3. Clin Invest Med 22: 195-206, 1999.
8. Deitch EA, Rutan R, Waymack JP. Trauma, shock, and gut translocation. New Horiz 4: 289-299, 1996.
9. Gawenis LR, Stien X, Shull GE, Schultheis PJ, Woo AL, Walker NM, Clarke LL. Intestinal NaCl transport in NHE2 and NHE3 knockout mice. Am J Physiol Gastrointest Liver Physiol 282: G776-G784, 2002.
10. Gens JS, Du H, Tackett L, Kong SS, Chu S, Montrose MH. Different ionic conditions prompt NHE2 and NHE3 translocation to the plasma membrane. Biochim Biophys Acta 1768: 1023-1035, 2007.
11. Gookin JL, Galanko JA, Blikslager AT, Argenzio RA. PG-mediated closure of paracellular pathway and not restitution is the primary determinant of barrier recovery in acutely injured porcine ileum. Am J Physiol Gastrointest Liver Physiol 285: G967-G979, 2003.
12. +-dependent acid extrusion mechanism is upregulated in NHE2-null mice. Am J Physiol Gastrointest Liver Physiol 291: G689-G699, 2006.
13. Lamprecht G, Weinman EJ, Yun CH. The role of NHERF and E3KARP in the cAMP-mediated inhibition of NHE3. J Biol Chem 273: 29972-29978, 1998.
14. + exchanger has no apparent effect on diarrheal state of NHE3-deficient mice. Am J Physiol Gastrointest Liver Physiol 281: G1385-G1396, 2001.
15. Little D, Dean RA, Young KM, McKane SA, Martin LD, Jones SL, Blikslager AT. PI3K signaling is required for prostaglandin-induced mucosal recovery in ischemia-injured porcine ileum. Am J Physiol Gastrointest Liver Physiol 284: G46-G56, 2003.
16. + exchanger isoform 2 stimulates recovery of barrier function in ischemia-injured intestine. Am J Physiol Gastrointest Liver Physiol 291: G885-G894, 2006.
17. Moore R, Carlson S, Madara JL. Rapid barrier restitution in an in vitro model of intestinal epithelial injury. Lab Invest 60: 237-244, 1989.
18. Nath SK, Kambadur R, Yun CH, Donowitz M, Tse CM. NHE2 contains subdomains in the COOH terminus for growth factor and protein kinase regulation. Am J Physiol Cell Physiol 276: C873-C882, 1999.
19. Nowak P, Blaheta R, Schuller A, Cinatl J, Wimmer-Greinecker G, Moritz A, Scholz M. The Na/H exchange inhibitor HOE642 prevents stress-induced epithelial barrier dysfunction. Int J Mol Med 14: 175-178, 2004.
20. Park PO, Haglund U, Bulkley GB, Falt K. The sequence of development of intestinal tissue injury after strangulation ischemia and reperfusion. Surgery 107: 574-580, 1990.
21. Sakakibara A, Furuse M, Saitou M, Ando-Akatsuka Y, Tsukita S. Possible involvement of phosphorylation of occludin in tight junction formation. J Cell Biol 137: 1393-1401, 1997.
22. + exchanger. Nat Genet 19: 282-285, 1998.
23. Stechmiller JK, Treloar D, Allen N. Gut dysfunction in critically ill patients: a review of the literature. Am J Crit Care 6: 204-209, 1997.
24. Sun F, Hug MJ, Lewarchik CM, Yun CH, Bradbury NA, Frizzell RA. E3KARP mediates the association of ezrin and protein kinase A with the cystic fibrosis transmembrane conductance regulator in airway cells. J Biol Chem 275: 29539-29546, 2000.
25. Turner JR. Molecular basis of epithelial barrier regulation: from basic mechanisms to clinical application. Am J Pathol 169: 1901-1909, 2006.
26. Turner JR, Angle JM, Black ED, Joyal JL, Sacks DB, Madara JL. PKC-dependent regulation of transepithelial resistance: roles of MLC and MLC kinase. Am J Physiol Cell Physiol 277: C554-C562, 1999.
27. + exchanger NHE3. Am J Physiol Cell Physiol 279: C1918-C1924, 2000.
28. Turner JR, Rill BK, Carlson SL, Carnes D, Kerner R, Mrsny RJ, Madara JL. Physiological regulation of epithelial tight junctions is associated with myosin light-chain phosphorylation. Am J Physiol Cell Physiol 273: C1378-C1385, 1997.
29. Wong V. Phosphorylation of occludin correlates with occludin localization and function at the tight junction. Am J Physiol Cell Physiol 273: C1859-C1867, 1997.
30. Yoo J, Nichols A, Mammen J, Calvo I, Song JC, Worrell RT, Matlin K, Matthews JB. Bryostatin-1 enhances barrier function in T84 epithelia through PKC-dependent regulation of tight junction proteins. Am J Physiol Cell Physiol 285: C300-C309, 2003.
31. + exchanger, NHE3, requires an associated regulatory protein. Proc Natl Acad Sci USA 94: 3010-3015, 1997.
32. + exchange. Annu Rev Physiol 67: 411-443, 2005.
33. + exchanger 3 (NHE3) are dependent on the presence but not the phosphorylation of NHE regulatory factor. J Biol Chem 274: 24753-24758, 1999.