Alteration of Bax-to-Bcl-2 ratio modulates the anticancer activity of methanolic extract of Commelina benghalensis (Commelinaceae) in Jurkat T cells

African Journal of Biotechnology

Volumn 7, Issue 20, 2008, Pages 3569-3576

  Mbazima, Vusi G ^a   Mokgotho, Matlou P ^a   February, Faghri ^b   Rees, D Jasper G ^b   Mampuru, Leseilane J ^a  

^a UNIVERSITY OF LIMPOPO   (South Africa)

^b UNIVERSITY OF THE WESTERN CAPE   (South Africa)

Author keywords

Apoptosis; Bax; Bcl 2; Commelina benghalensis; Jurkat T cells; p53

Indexed keywords

ANTINEOPLASTIC AGENT; COMMELINA BENGHALENSIS EXTRACT; METHANOL; PLANT EXTRACT; PROTEIN BAX; PROTEIN BCL 2; PROTEIN P53; UNCLASSIFIED DRUG;

ANTINEOPLASTIC ACTIVITY; APOPTOSIS; ARTICLE; CANCER INHIBITION; CELL VIABILITY; COMMELINA; COMMELINA BENGHALENSIS; CONCENTRATION RESPONSE; DRUG CYTOTOXICITY; DRUG ISOLATION; ETHNOPHARMACOLOGY; HUMAN; HUMAN CELL; LEUKEMIA CELL LINE; MEDICINAL PLANT; PLANT STEM; PROTEIN EXPRESSION; REVERSE TRANSCRIPTION POLYMERASE CHAIN REACTION; WESTERN BLOTTING;

COMMELINA BENGHALENSIS; COMMELINACEAE;

EID: 57349118424     PISSN: None     EISSN: 16845315     Source Type: Journal    
DOI: None     Document Type: Article

References (33)

1. Chinnaiyan A, Dixit V (1996). The cell-death machine. Curr. Biol. 6: 555-562.
2. Cohen GM (1997). Caspases: the executioners of apoptosis. Biochem. J. 326: 1-6.
3. Cox PA (1994). The ethnobotanical approach to drug discovery: strength and limitations. In ethnobotany and the search for new drug. Ciba Foundation Symposium 185. Chichester: John Wiley and Sons, pp25-41.
4. Denault JB, Salvesen GS (2002). Caspases: keys in the ignition of cell death. Chem. Rev. 102(12): 4489-4500.
5. Farnsworth NR (1984). The role of medicinal plants in drug development. In: Krogsgaard-larsen P, Christensen SB, Kofod H (eds). Natural products and drug development. London: Balliere Tindall and Cox, pp8-98.
6. Hofseth LJ, Hussain SP, Harris CC (2004). p53: 25 years after its discovery. Trends Pharmacol. Sci. 25(4): 177-181.
7. Hong DY, DeFillipps RA (2000). Commelina diffusa, in Wu ZY, Raven PH, Hong DY (eds), Flora of China Vol. 24, Beijing: Science Press, St. Louis, Missouri Garden Press, p. 36.
8. Huang S-T, Cidlowski JA (2002). Phosphorylation status modulates Bcl-2 function during glucocorticoid-induced apoptosis in T lymphocytes. FASEB J. 16: 825-832.
9. http://botanical.com/site/column_poudhia/articles/_11840.html, 2008/02/07
10. Israels LG, Israels ED (1999). Apoptosis. Stem Cells 17: 306-313.
11. Kasibhatla S, Tseng B (2003). Minireview: Why target apoptosis in cancer treatment. Mol. Cancer Ther. 2: 573-580.
12. Lau CBS, Ho CY, Kim CF, Leung KN, Fung KP, Tse TF, Chan HHL, Chow MSS (2004). Cytotoxic activity of Coriolus versicolor (Yunzhi) extract on human leukaemia and lymphoma cells by induction of apoptosis. Life Sci. 75: 797-808.
13. Lee YJ, Kuo HC, Chu CY, Wang CJ, Lin WC, Tseng TH (2003). Involvement of tumor suppressor protein p53 and p38 MAKP in caffeic acid phenethyl ester-induced apoptosis of C6 glioma cells. Biochem. Pharmacol. 66: 2281-2289.
14. Leist M, Jaattela M (2001). For deaths and a funeral: from caspases to alternative mechanisms. Nat. Rev. Mol. Cell Biol. 2(8): 589-598.
15. 2-M phase arrest and apoptosis. Mol. Cancer Ther. 1: 841-849.
16. Liu S, Li J, Tao Y, Xiao X (2007). Small heat shock protein αB-crystallin binds to p53 to sequester its translocation to mitochondria during hydrogen peroxide-induced apoptosis. Biochem. Biophys. Res. Comm. 354: 109-114.
17. Miyoshi N, Nakamura Y, Ueda Y, Abe M, Ozawa Y, Uchida K, Osawa T (2003). Dietary Ginger constituents galanals A and B, are potent apoptosis inducers in human T lymphoma Jurkat T cells. Cancer Lett. 199: 113-119.
18. Orrenius S (2004). Mitochondrial regulation of apoptotic cell death. Toxicol. Lett. 149: 19-23.
19. Pfaffl MW, Horgan GW, Dempfle L (2002). Relative Expression Software Tool (REST©) for groupwise comparison and statistical analysis of relative expression results in real-time PCR. Nucleic Acids Res. 30: e36.
20. Qaiser M, Jafri SMH (1975). Commelina benghalensis, in Ali SI, Qaiser M (eds), Flora of Pakistan, Vol. 84, St. Louis: University of Karachi & Missouri Botanical Garden, pp10.
21. Scatena CD, Stewart ZA, Mays D, Tang LJ, Keefer CJ, Leach SD, Pietenpol JA (1998). Mitotic phosphorylation of Bcl-2 during normal cell cycle progression and taxol-induced growth arrest. J. Biol. Chem. 273(46): 30777-30784.
22. Seraste A, Pulkki K (2000). Morphologic and biochemical hallmarks of apoptosis. Cardiov ascular Res. 45(3): 528-537.
23. Sutcliffe JE, Brehm A (2004). Of flies and men; p53, a tumour suppressor. FEBS Lett. 567: 86-91.
24. Taraphdar AK, Roy M, Bhattacharya RK (2001). Natural products as inducers of apoptosis: Implication for cancer therapy and prevention. Curr. Sci. 80(11): 1387-1396.
25. Taylor JLS, Rabe T, McGaw LJ, Jager AK, van Staden J (2001). Towards the scientific validation of traditional medicinal plants. Plant Growth Reg. 34: 23-37.
26. Thomas A, Giesler T, White E (2000). p53 mediates Bcl-2 phosphorylation and apoptosis via activation of the Cdc42/JNK1 pathway. Oncogene 19: 5259-5269.
27. Vander HMG, Thompson CB (1999). Bcl-2 proteins regulators of apoptosis or cell mitochondrial homeostasis? Nat. Cell Biol. 1: E209-216.
28. Vandesompele J, De Preter K, Pattyn F, Poppe B, Van Roy N, De Paepe A, Spelema F (2002). Accurate normalization of real-time quantitative RT-PCR data by geometric averaging of multiple internal control genes. Gen. Biol. 3: 1-12.
29. Xie J, Shaikh ZA (2006). Cadmium induces cell cycle arrest in rat kidney epithelial cells in G2/M phase. Toxicol. 224: 56-65.
30. Yang LL, Lee CY, Yen KY (2000). Induction of apoptosis by hydrolysable tannins from Eugenia jambos L. on human leukaemia cells. Cancer Lett. 175: 65-75.
31. Yi JM, Kim MS, Lee EH, Wi DH, Lee JK, Cho KH, Hong SH, Kim HM (2003). Induction of apoptosis by Paljin-Hangahmdan on human leukemia cells. J. Ethnopharmacol. 88: 79-83.
32. Zimmerman KC, Bonzon C, Green DR (2001). The machinery of cell death. Pharmacol. Ther. 92: 57-70.
33. Zörnig M, Hueber AO, Baum W, Evan G (2001). Review: Apoptosis regulators and their role in tumorigenesis. Biochim. Biophys. Acta 1551: F1-F37.