Probing the catalytic center of porcine aminoacylase 1 by site-directed mutagenesis, homology modeling and substrate docking

Journal of Biochemistry

Volumn 139, Issue 3, 2006, Pages 421-430

  Liu, Zhigang ^a   Zhen, Zhongliang ^a   Zuo, Zhenyu ^a   Wu, Yingliang ^a   Liu, Aifu ^a   Yi, Qingming ^a   Li, Wenxin ^a  

^a WUHAN UNIVERSITY   (China)

Author keywords

Aminoacylase 1; Catalytic center; Docking; Energy minimization; Homology modeling; Site directed mutagenesis

Indexed keywords

AMINOACYLASE; ARGININE; ASPARTIC ACID; DOCKING PROTEIN; GLUTAMIC ACID; HISTIDINE; PEPTIDASE; ZINC ION;

ARTICLE; CATALYSIS; CONTROLLED STUDY; CRYSTAL STRUCTURE; ENERGY YIELD; ENZYME ACTIVITY; ENZYME SUBSTRATE COMPLEX; GENE MUTATION; GENE OVEREXPRESSION; NONHUMAN; PROTEIN BINDING; PROTEIN EXPRESSION; PROTEIN STRUCTURE; SEQUENCE HOMOLOGY; SITE DIRECTED MUTAGENESIS; WILD TYPE;

AMIDOHYDROLASES; AMINO ACID SEQUENCE; AMINO ACID SUBSTITUTION; ANIMALS; CATALYSIS; CATALYTIC DOMAIN; MODELS, MOLECULAR; MOLECULAR SEQUENCE DATA; MUTAGENESIS, SITE-DIRECTED; SEQUENCE HOMOLOGY, AMINO ACID; SUBSTRATE SPECIFICITY; SWINE;

SUIDAE;

EID: 33646365639     PISSN: 0021924X     EISSN: None     Source Type: Journal    
DOI: 10.1093/jb/mvj047     Document Type: Article

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