Quantitative optimization of reverse transfection conditions for 384-well siRNA library screening

Assay and Drug Development Technologies

Volumn 6, Issue 5, 2008, Pages 683-691

  LaPan, Peter ^a   Zhang, Jing ^a   Pan, Jing ^a   Haney, Steve ^a,b  

^a PFIZER INC   (United States)

^b PFIZER GLOBAL RESEARCH AND DEVELOPMENT   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

GLYCERALDEHYDE 3 PHOSPHATE DEHYDROGENASE; LIPID; SMALL INTERFERING RNA;

ARTICLE; DRUG CYTOTOXICITY; ENZYMATIC ASSAY; GENETIC TRANSFECTION; HUMAN; HUMAN CELL; QUANTITATIVE ANALYSIS; RNA ANALYSIS;

BASE SEQUENCE; CELL COUNT; CELL LINE, TUMOR; DATA INTERPRETATION, STATISTICAL; GENE LIBRARY; GLYCERALDEHYDE-3-PHOSPHATE DEHYDROGENASES; HUMANS; INDICATORS AND REAGENTS; LIPIDS; REPRODUCIBILITY OF RESULTS; REVERSE TRANSCRIPTASE POLYMERASE CHAIN REACTION; RNA, SMALL INTERFERING; TRANSFECTION;

EID: 57049109361     PISSN: 1540658X     EISSN: None     Source Type: Journal    
DOI: 10.1089/adt.2008.142     Document Type: Article

References (17)

1. Elbashir SM, Harborth J, Lendeckel W, Yalcin A, Weber K, Tuschl T: Duplexes of 21-nucleotide RNAs mediate RNA interference in cultured mammalian cells. Nature 2001;411:494-498.
2. Aza-Blanc P, Cooper CL, Wagner K, Batalov S, Deveraux QL, Cooke MP: Identification of modulators of TRAIL-induced apoptosis via RNAi-based phenotypic screening. Mol Cell 2003;12:627-637.
3. Berns K, Hijmans EM, Mullenders J, Brummelkamp TR, Velds A, Heimerikx M, et al.: A large-scale RNAi screen in human cells identifies new components of the p53 pathway. Nature 2004;428:431-437.
4. Paddison PJ, Silva JM, Conklin DS, Schlabach M, Li MM, Aruleba S, et al.: A resource for large-scale RNA-interference-based screens in mammals. Nature 2004;428:427-431.
5. Aoki M, Ishii T, Kanaoka M, Kimura T: RNA interference in immune cells by use of osmotic delivery of siRNA. Biochem Biophys Res Commun 2006;341:326-333.
6. Bonetta L: The inside scoop - evaluating gene delivery methods. Nat Methods 2005;2:875-883.
7. Borawski J, Lindeman A, Buxton F, Labow M, Gaither LA: Optimization procedure for small interfering RNA transfection in a 384-well format. J Biomol Screen 2007;12:546-559.
8. Chiu YL, Ali A, Chu CY, Cao H, Rana TM: Visualizing a correlation between siRNA localization, cellular uptake, and RNAi in living cells. Chem Biol 2004;11:1165-1175.
9. Rozema DB, Lewis DL: siRNA delivery technologies for mammalian systems. TARGETS 2003;2:253-260.
10. Simeoni F, Morris MC, Heitz F, Divita G: Insight into the mechanism of the peptide-based gene delivery system MPG: implications for delivery of siRNA into mammalian cells. Nucleic Acids Res 2003;31:2717-2724.
11. Mousses S, Caplen NJ, Cornelison R, Weaver D, Basik M, Hautaniemi S, et al.: RNAi microarray analysis in cultured mammalian cells. Genome Res 2003;13:2341-2347.
12. Chanda SK, White S, Orth AP, Reisdorph R, Miraglia L, Thomas RS, et al.: Genome-scale functional profiling of the mammalian AP-1 signaling pathway. Proc Natl Acad Sci U S A 2003;100:12153-12158.
13. Husken D, Asselbergs F, Kinzel B, Natt F, Weiler J, Martin P, et al.: mRNA fusion constructs serve in a general cell-based assay to profile oligonucleotide activity. Nucleic Acids Res 2003;31:e102.
14. Kumar R, Conklin DS, Mittal V: High-throughput selection of effective RNAi probes for gene silencing. Genome Res 2003;13:2333-2340.
15. KDAlert GAPDH Manual, Version 0601, pp. 1-21. Ambion, Inc., Foster City, CA, 2006.
16. Spankuch-Schmitt B, Bereiter-Hahn J, Kaufmann M, Strebhardt K: Effect of RNA silencing of polo-like kinase-1 (PLK1) on apoptosis and spindle formation in human cancer cells. J Natl Cancer Inst 2002;94:1863-1877.
17. Zhang JH, Chung TD, Oldenburg KR: A simple statistical parameter for use in evaluation and validation of high throughput screening assays. J Biomol Screen 1999;4:67-73.