ALG-2 knockdown in HeLa cells results in G2/M cell cycle phase accumulation and cell death

Biochemical and Biophysical Research Communications

Volumn 378, Issue 1, 2009, Pages 145-148

  Høj, Berit R ^a   la Cour, Jonas M ^a   Mollerup, Jens ^a   Berchtold, Martin W ^a  

^a UNIVERSITY OF COPENHAGEN   (Denmark)

Author keywords

Apoptosis; Apoptosis linked gene 2 (ALG 2); Calcium binding proteins; Caspase; Cell cycle; siRNA

Indexed keywords

APOPTOSIS LINKED GENE 2; CALCIUM BINDING PROTEIN; UNCLASSIFIED DRUG;

APOPTOSIS; ARTICLE; CANCER CELL; CELL CYCLE G2 PHASE; CELL CYCLE M PHASE; DOWN REGULATION; HELA CELL; HUMAN; HUMAN CELL; NUCLEOTIDE SEQUENCE; PRIORITY JOURNAL; WESTERN BLOTTING;

APOPTOSIS; APOPTOSIS REGULATORY PROTEINS; CALCIUM-BINDING PROTEINS; CASPASES; CELL DIVISION; DOWN-REGULATION; G2 PHASE; GENE KNOCKDOWN TECHNIQUES; HELA CELLS; HUMANS; NEOPLASMS;

EID: 56949105260     PISSN: 0006291X     EISSN: 10902104     Source Type: Journal    
DOI: 10.1016/j.bbrc.2008.11.021     Document Type: Article

References (21)

1. Vito P., Lacana E., and D'Adamio L. Interfering with apoptosis: Ca(2+)-binding protein ALG-2 and Alzheimer's disease gene ALG-3. Science 271 (1996) 521-525
2. Tarabykina S., Moller A.L., Durussel I., Cox J., and Berchtold M.W. Two forms of the apoptosis-linked protein ALG-2 with different Ca(2+) affinities and target recognition. J. Biol. Chem. 275 (2000) 10514-10518
3. Tarabykina S., Mollerup J., Winding P., and Berchtold M.W. ALG-2, a multifunctional calcium binding protein?. Front. Biosci. 9 (2004) 1817-1832
4. Lacana E., Ganjei J.K., Vito P., and D'Adamio L. Dissociation of apoptosis and activation of IL-1beta-converting enzyme/Ced-3 proteases by ALG-2 and the truncated Alzheimer's gene ALG-3. J. Immunol. 158 (1997) 5129-5135
5. Rao R.V., Poksay K.S., Castro-Obregon S., Schilling B., Row R.H., del Rio G., Gibson B.W., Ellerby H.M., and Bredesen D.E. Molecular components of a cell death pathway activated by endoplasmic reticulum stress. J. Biol. Chem. 279 (2004) 177-187
6. Mahul-Mellier A.L., Hemming F.J., Blot B., Fraboulet S., and Sadoul R. Alix, making a link between apoptosis-linked gene-2, the endosomal sorting complexes required for transport, and neuronal death in vivo. J. Neurosci. 26 (2006) 542-549
7. Mollerup J., Krogh T.N., Nielsen P.F., and Berchtold M.W. Properties of the co-chaperone protein p23 erroneously attributed to ALG-2 (apoptosis-linked gene 2). FEBS Lett. 555 (2003) 478-482
8. la Cour J.M., Mollerup J., Winding P., Tarabykina S., Sehested M., and Berchtold M.W. Up-regulation of ALG-2 in hepatomas and lung cancer tissue. Am. J. Pathol. 163 (2003) 81-89
9. la Cour J.M., Høj B.R., Mollerup J., simon R., Sauter G., and Berchtold M.W. The apoptosis linked gene ALG-2 is dysregulated in tumors of various origin and contributes to cancer cell viability. Mol. Oncol. 1 (2008) 431-439
10. Aviel-Ronen S., Coe B.P., Lau S.K., da Cunha Santos G., Zhu C.Q., Strumpf D., Jurisica I., Lam W.L., and Tsao M.S. Genomic markers for malignant progression in pulmonary adenocarcinoma with bronchioloalveolar features. Proc. Natl. Acad. Sci. USA 105 (2008) 10155-10160
11. la Cour J.M., Mollerup J., and Berchtold M.W. ALG-2 oscillates in subcellular localization, unitemporally with calcium oscillations. Biochem. Biophys. Res. Commun. 353 (2007) 1063-1067
12. Elbashir S.M., Harborth J., Lendeckel W., Yalcin A., Weber K., and Tuschl T. Duplexes of 21-nucleotide RNAs mediate RNA interference in cultured mammalian cells. Nature 411 (2001) 494-498
13. Vermes I., Haanen C., Steffens-Nakken H., and Reutelingsperger C. A novel assay for apoptosis. Flow cytometric detection of phosphatidylserine expression on early apoptotic cells using fluorescein labelled Annexin V. J. Immunol. Methods 184 (1995) 39-51
14. Burns T.F., Fei P., Scata K.A., Dicker D.T., and El-Deiry W.S. Silencing of the novel p53 target gene Snk/Plk2 leads to mitotic catastrophe in paclitaxel (taxol)-exposed cells. Mol. Cell. Biol. 23 (2003) 5556-5571
15. Clute P., and Pines J. Temporal and spatial control of cyclin B1 destruction in metaphase. Nat. Cell Biol. 1 (1999) 82-87
16. Castedo M., Perfettini J.L., Roumier T., Andreau K., Medema R., and Kroemer G. Cell death by mitotic catastrophe: a molecular definition. Oncogene 23 (2004) 2825-2837
17. Wong D.C., Wong K.T., Nissom P.M., Heng C.K., and Yap M.G. Targeting early apoptotic genes in batch and fed-batch CHO cell cultures. Biotechnol. Bioeng. 95 (2006) 350-361
18. Czauderna F., Santel A., Hinz M., Fechtner M., Durieux B., Fisch G., Leenders F., Arnold W., Giese K., Klippel A., and Kaufmann J. Inducible shRNA expression for application in a prostate cancer mouse model. Nucleic Acids Res. 31 (2003) e127
19. 2+-dependent manner. Biochem. Biophys. Res. Commun. 353 (2007) 756-763
20. 2+-binding protein ALG-2 is recruited to endoplasmic reticulum exit sites by Sec31A and stabilizes the localization of sec31A. Mol. Biol. Cell 17 (2006) 4876-4887
21. Jang I.K., Hu R., Lacana E., D'Adamio L., and Gu H. Apoptosis-linked gene 2-deficient mice exhibit normal T-cell development and function. Mol. Cell. Biol. 22 (2002) 4094-4100