Expression and function of system N glutamine transporters (SN1/SN2 or SNAT3/SNAT5) in retinal ganglion cells

Investigative Ophthalmology and Visual Science

Volumn 49, Issue 11, 2008, Pages 5151-5160

  Umapathy, Nagavedi S ^a   Dun, Ying ^a   Martin, Pamela M ^a   Duplantier, Jennifer N ^a   Roon, Penny ^a   Prasad, Puttur ^a   Smith, Sylvia B ^a   Ganapathy, Vadivel ^a  

^a MEDICAL COLLEGE OF GEORGIA   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

GLUTAMIC ACID; GLUTAMINE; MESSENGER RNA; PROTEIN; SN1 PROTEIN; SN2 PROTEIN; SNAT3 PROTEIN; SNAT5 PROTEIN; UNCLASSIFIED DRUG; VIMENTIN; AGENTS INTERACTING WITH TRANSMITTER, HORMONE OR DRUG RECEPTORS; AMINO ACID TRANSPORTER; SLC38A5 PROTEIN, HUMAN; SYSTEM N PROTEIN 1;

ANIMAL CELL; ANIMAL TISSUE; ARTICLE; CELL TYPE; GENE; HUMAN; HUMAN CELL; IMMUNOFLUORESCENCE; IN SITU HYBRIDIZATION; MOUSE; NONHUMAN; NUCLEOTIDE SEQUENCE; PHOTORECEPTOR CELL; PRIORITY JOURNAL; PROTEIN ANALYSIS; PROTEIN EXPRESSION; PROTEIN FUNCTION; RAT; RETINA; RETINA BIPOLAR GANGLION CELL; RETINA GANGLION CELL; REVERSE TRANSCRIPTION POLYMERASE CHAIN REACTION; RNA ANALYSIS; WESTERN BLOTTING; ANIMAL; BIOSYNTHESIS; CELL CULTURE; COMPARATIVE STUDY; CYTOLOGY; GENE EXPRESSION; GENETICS; METABOLISM; SIGNAL TRANSDUCTION;

AMINO ACID TRANSPORT SYSTEMS, NEUTRAL; ANIMALS; BLOTTING, WESTERN; CELLS, CULTURED; GENE EXPRESSION; IN SITU HYBRIDIZATION; MICE; NEUROTRANSMITTER AGENTS; RETINAL GANGLION CELLS; REVERSE TRANSCRIPTASE POLYMERASE CHAIN REACTION; RNA, MESSENGER; SIGNAL TRANSDUCTION;

EID: 56149091597     PISSN: 01460404     EISSN: 15525783     Source Type: Journal    
DOI: 10.1167/iovs.08-2245     Document Type: Article

References (36)

1. Kalloniatis M, Tomisich G. Amino acid neurochemistry of the vertebrate retina. Prog Retin Eye Res. 1999;18:811-866. (Pubitemid 29445802)
2. Pow DV. Amino acids and their transporters in the retina. Neurochem Int. 2001;38:463-484. (Pubitemid 32204358)
3. Thoreson WB, Witkovsky P. Glutamate receptors and circuits in the vertebrate retina. Prog Retin Eye Res. 1999;18:765-810. (Pubitemid 29445801)
4. Ganapathy V, Inoue K, Prasad PD, Ganapathy ME. Cellular uptake of amino acids: systems and regulation. In: Cynober LA, ed. Metabolic and Therapeutic Aspects of Amino Acids in Clinical Nutrition. 2nd ed. Boca Raton, FL: CRC Press; 2004:63-78.
5. Mackenzie B, Erickson JD. Sodium-coupled neutral amino acid (system N/A) transporters of the SLC38 gene family. Pflugers Arch Eur J Physiol. 2004;447,784-795. (Pubitemid 38241468)
6. Verrey F. System L: heteromeric exchangers of large, neutral amino acids involved in directional transport. Pflugers Arch Eur J Physiol. 2003;445:529-533. (Pubitemid 36286884)
7. Hatanaka T, Huang W, Wang H, et al. Primary structure, functional characteristics and tissue expression pattern of human ATA2, a subtype of amino acid transport system A. Biochim Biophys Acta. 2000;1467:1-6. (Pubitemid 30621579)
8. Sugawara M, Nakanishi T, Fei YJ, et al. Cloning of an amino acid transporter with functional characteristics and tissue expression pattern identical to that of system A. J Biol Chem. 2000;275:16473-16477. (Pubitemid 30398868)
9. Varoqui H, Zhu H, Yao D, Ming H, Erickson JD. Cloning and functional identification of a neuronal glutamine transporter. J Biol Chem. 2000;275,4049-4054. (Pubitemid 30094634)
10. Wang H, Huang W, Sugawara M, et al. Cloning and functional expression of ATA1, a subtype of amino acid transporter A, from human placenta. Biochem Biophys Res Commun. 2000;273,1175-1179. (Pubitemid 30481098)
11. Chaudhry FA, Reimer RJ, Krizaj D, et al. Molecular analysis of system N suggests novel physiological roles in nitrogen metabolism and synaptic transmission. Cell. 1999;99:769-780. (Pubitemid 30017647)
12. +-coupled glutamine transporter. J Biol Chem. 2000;275:23707-23717.
13. Nakanishi T, Kekuda R, Fei YJ, et al. Cloning and functional characterization of a new subtype of the amino acid transport system N. Am J Physiol. 2000;281:C1757-C1768.
14. Nakanishi T, Sugawara M, Huang W, et al. Structure, function, and tissue expression pattern of human SN2, a subtype of the amino acid transport system N. Biochem Biophys Res Commun. 2001;281:1343-1348. (Pubitemid 32924567)
15. Christensen HN. Distinguishing amino acid transport systems of a given cell or tissue. Methods Enzymol. 1989;173,576-616. (Pubitemid 19238570)
16. Verrey F, Closs EI, Wagner CA, Palacin M, Endou H, Kanai Y. CATs and HATs: the SLC7 family of amino acid transporters. Pflugers Arch. 2004;447:532-542. (Pubitemid 38258215)
17. Kanai Y, Hediger MA. The glutamate and neutral amino acid transporter family: physiological and pharmacological implications. Eur J Pharmacol. 2003;479:237-247. (Pubitemid 37377892)
18. Sloan JL, Mager S. Cloning and functional expression of a human Na(+) and Cl(-)-dependent neutral and cationic amino acid transporter B(0+). J Biol Chem. 1999;274:23740-23745. (Pubitemid 129528409)
19. Chaudhry FA, Reimer RJ, Edwards RH. The glutamine commute: take the N line and transfer to the A. J Cell Biol. 2002;157:349-355.
20. Cubelos B, González-González IM, Giménez C, Zafra F. Amino acid transporter SNAT5 localizes to glial cells in the rat brain. Glia. 2005;49:230-244. (Pubitemid 40188502)
21. Edwards RH. The neurotransmitter cycle and quantal size. Neuron. 2007;55:835-858.
22. Melone M, Quagliano F, Barbaresi P, Varoqui H, Erickson JD, Conti F. Localization of the glutamine transporter SNAT1 in rat cerebral cortex and neighboring structures, with a note on its localization in human cortex. Cereb Cortex. 2004;14:562-574. (Pubitemid 38579657)
23. +-coupled neutral amino acid transporter 2 in the cerebral cortex. Neuroscience. 2006;140:281-292.
24. Sáenz DA, Goldin AP, Minces L, Chianelli M, Sarmiento MI, Rosenstein RE. Effect of melatonin on the retinal glutamate/glutamine cycle in the golden hamster retina. FASEB J. 2004 18;1912-1913. (Pubitemid 39602281)
25. Boulland JL, Osen KK, Levy LM, et al. Cell-specific expression of the glutamine transporter SN1 suggests differences in dependence on the glutamine cycle. Eur J Neurosci. 2002;15:1615-1631.
26. Umapathy NS, Li W, Mysona BA, Smith SB, Ganapathy V. Expression and function of glutamine transporters SN1 (SNAT3) and SN2 (SNAT5) in retinal Müller cells. Invest Ophthalmol Vis Sci. 2005;46:3980-3987. (Pubitemid 44264596)
27. Gu S, Roderick HL, Camacho P, Jiang JX. Characterization of an N-system amino acid transporter expressed in retina and its involvement in glutamine transport. J Biol Chem. 2001;276:24137-24144. (Pubitemid 37410819)
28. Krishnamoorthy RR, Agarwal P, Prasanna G, et al. Characterization of a transformed rat retinal ganglion cell line. Brain Res Mol Brain Res. 2001;86:1-12. (Pubitemid 32124753)
29. Dun Y, Duplantier J, Roon P, Martin PM, Ganapathy V, Smith SB. Serine racemase expression and D-serine content are developmentally regulated in neuronal ganglion cells of the retina. J Neurochem. 2008;104:970-978. (Pubitemid 351143637)
30. -) in retinal ganglion cells and regulation by nitric oxide and oxidative stress. Cell Tissue Res. 2006;324:189-202.
31. Dun Y, Thangaraju M, Prasad P, Ganapathy V, Smith SB. Prevention of excitotoxicity in primary retinal ganglion cells by (+)-pentazocine, a sigma receptor-1 specific ligand. Invest Ophthalmol Vis Sci. 2007;48:4785-4794. (Pubitemid 351260999)
32. Barnstable CJ, Dräger UC. Thy-1 antigen: a ganglion cell specific marker in rodent retina. Neuroscience. 1984;11:847-855.
33. Shaw G, Weber K. The structure and development of the rat retina: an immunofluorescence microscopical study using antibodies specific for intermediate filament proteins. Eur J Cell Biol. 1983;30:219-232.
34. Lowry OH, Rosebrough NJ, Farr AL, Randall RJ. Protein measurement with the folin-phenol reagents. J Biol Chem. 1951;193,265-275.
35. Solbu TT, Boulland JL, Zahid W, et al. Induction and targeting of the glutamine transporter SN1 to the basolateral membranes of cortical kidney tubule cells during chronic metabolic acidosis suggest a role in pH regulation. J Am Soc Nephrol. 2005;16:869-877. (Pubitemid 41710297)
36. Conti F, Melone M. The glutamine commute: lost in the tube? Neurochem Int. 2006;48:459-464.