Identification of lysine residues critical for the transcriptional activity and polyubiquitination of the NF-κB family member RelB

Biochemical Journal

Volumn 416, Issue 1, 2008, Pages 117-127

  Leidner, Julia ^a   Palkowitsch, Lysann ^a   Marienfeld, Uta ^a   Fischer, Dietmar ^a   Marienfeld, Ralf ^a  

^a UNIVERSITY OF ULM   (Germany)

Author keywords

Co factor; Gene expression; Nuclear factor B (NF B); Protein stability; RelB; Ubiquitination

Indexed keywords

293 CELLS; B CELLS; CELL LINES; CO-FACTOR; DEATH DOMAIN; DNA BINDING; DNA METHYLTRANSFERASE; DUAL ROLE; HUMAN EMBRYONIC KIDNEYS; IONOMYCIN; KEY COMPONENT; LYSINE RESIDUES; MOLECULAR MECHANISM; NUCLEAR FACTORS; NUCLEAR LOCALIZATION; POLYUBIQUITINATION; PROTEASOMAL DEGRADATION; PROTEIN STABILITY; RELB; SIGNALLING PATHWAYS; TARGET GENES; TARGET SITES; TRANSCRIPTIONAL ACTIVITY; UBIQUITIN; UBIQUITINATION;

AMINO ACIDS; BIOACTIVITY; CELL CULTURE; DNA; GENES; NUCLEIC ACIDS; TRANSCRIPTION FACTORS;

TRANSCRIPTION;

IONOMYCIN; LYSINE; PHORBOL 13 ACETATE 12 MYRISTATE; TRANSCRIPTION FACTOR RELB; UBIQUITIN;

ARTICLE; B LYMPHOCYTE; CELL NUCLEUS; CELL STRAIN HEK293; CONTROLLED STUDY; GENE EXPRESSION REGULATION; HUMAN; HUMAN CELL; PRIORITY JOURNAL; PROTEIN DEGRADATION; PROTEIN DNA BINDING; PROTEIN LOCALIZATION; PROTEIN PROCESSING; PROTEIN STABILITY; REGULATORY MECHANISM; SIGNAL TRANSDUCTION; TRANSACTIVATION; TRANSCRIPTION REGULATION; UBIQUITINATION; CELL LINE; CHEMISTRY; GENETIC TRANSCRIPTION; KIDNEY; METABOLISM; PHYSIOLOGY; PRENATAL DEVELOPMENT;

CELL LINE; GENE EXPRESSION REGULATION; HUMANS; KIDNEY; LYSINE; TRANSCRIPTION FACTOR RELB; TRANSCRIPTION, GENETIC; UBIQUITIN; UBIQUITINATION;

EID: 56049119389     PISSN: 02646021     EISSN: 14708728     Source Type: Journal    
DOI: 10.1042/BJ20080432     Document Type: Article

References (24)

1. Hayden, M. S. and Ghosh, S. (2004) Signaling to NF-κB. Genes Dev. 18, 2195-2224
2. Ghosh, S. and Karin, M. (2002) Missing pieces in the NF-κB puzzle. Cell 109, S81-S96
3. Senftleben, U., Cao, Y., Xiao, G., Greten, F. R., Krahn, G., Bonizzi, G., Chen, Y., Hu, Y., Fong, A., Sun, S. C. and Karin, M. (2001) Activation by IKKα of a second, evolutionary conserved, NF-κB signaling pathway. Science 293, 1495-1499
4. Saccani, S., Pantano, S. and Natoli, G. (2003) Modulation of NF-κB activity by exchange of dimers. Mol. Cell 11, 1563-1574
5. Croxton, R., Puto, L. A., de Belle, I., Thomas, M., Torii, S., Hanaii, F., Cuddy, M. and Reed, J. C. (2006) Daxx represses expression of a subset of anti-apoptotic genes regulated by nuclear factor-κB. Cancer Res. 66, 9026-9035
6. Chen, L. F., Mu, Y. and Greene, W. C. (2002) Acetylation of RelA at discrete sites regulates distinct nuclear functions of NF-κB. EMBO J. 21, 6539-6548
7. Zhong, H., May, M. J., Jimi, E. and Ghosh, S. (2002) The phosphorylation status of nuclear NF-κB determines its association with CBP/p300 or HDAC-1. Mol. Cell 9, 625-636
8. Duran, A., Diaz-Meco, M. T. and Moscat, J. (2003) Essential role of RelA Ser311 phosphorylation by ζ PKC in NF-κB transcriptional activation. EMBO J. 22, 3910-3918
9. Adhikary, S., Marinoni, F., Hock, A., Hulleman, E., Popov, N., Beier, R., Bernard, S., Quarto, M., Capra, M., Goettig, S. et al. (2005) The ubiquitin ligase HectH9 regulates transcriptional activation by Myc and is essential for tumor cell proliferation. Cell 123, 409-421
10. Marienfeld, R., Berberich-Siebelt, F., Berberich, I., Denk, A., Serfling, E. and Neumann, M. (2001) Signal-specific and phosphorylation-dependent RelB degradation: a potential mechanism of NF-κB control. Oncogene 20, 8142-8147
11. Marienfeld, R., May, M. J., Berberich, I., Serfling, E., Ghosh, S. and Neumann, M. (2003) RelB forms transcriptionally inactive complexes with RelA/p65. J. Biol. Chem. 278, 19852-19860
12. Maier, H. J., Marienfeld, R., Wirth, T. and Baumann, B. (2003) Critical role of RelB serine 368 for dimerization and p100 stabilization. J. Biol. Chem. 278, 39242-39250
13. Oeckinghaus, A., Wegener, E., Welteke, V., Ferch, U., Arslan, S. C., Ruland, J., Scheidereit, C. and Krappmann, D. (2007) Malt1 ubiquitination triggers NF-κB signaling upon T-cell activation. EMBO J. 26, 4634-4645
14. Palkowitsch, L., Leidner, J., Ghosh, S. and Marienfeld, R. B. (2008) The phosphorylation of serine 68 in the IKK-binding domain of NEMO interferes with the structure of the IKK-complex and the TNF-α -induced NF-κB activity. J. Biol. Chem. 283, 76-86
15. Baumann, B., Kistler, B., Kirillov, A., Bergman, Y. and Wirth, T. (1998) The mutant plasmacytoma cell line S107 allows the identification of distinct pathways leading to NF-κB activation. J. Biol. Chem. 273, 11448-11455
16. Weih, F., Carrasco, D. and Bravo, R. (1994) Constitutive and inducible Rel/NF-κB activities in mouse thymus and spleen. Oncogene 9, 3289-3297
17. Lernbecher, T., Kistler, B. and Wirth, T. (1994) Two distinct mechanisms contribute to the constitutive activation of RelB in lymphoid cells. EMBO J. 13, 4060-4069
18. Lernbecher, T., Muller, U. and Wirth, T. (1993) Distinct NF-κB/Rel transcription factors are responsible for tissue-specific and inducible gene activation. Nature 365, 767-770
19. Lawrence, T., Bebien, M., Liu, G. Y., Nizet, V. and Karin, M. (2005) IKKα limits macrophage NF-κB activation and contributes to the resolution of inflammation. Nature 434, 1138-1143
20. Carmody, R. J., Ruan, Q., Palmer, S., Hilliard, B. and Chen, Y. H. (2007) Negative regulation of toll-like receptor signaling by NF-eB p50 ubiquitination blockade. Science 317, 675-678
21. Haglund, K. and Dikic, I. (2005) Ubiquitylation and cell signaling. EMBO J 24, 3353-3359
22. Ikeda, F. and Dikic, I. (2008) Atypical ubiquitin chains: new molecular signals. EMBO Rep. 9, 536-542
23. Tang, E. D., Wang, C. Y., Xiong, Y. and Guan, K. L. (2003) A role for NF-κB essential modifier/IκB kinaseγ (NEMO/IKKγ) ubiquitination in the activation of the IκB kinase complex by tumor necrosis factor-α. J. Biol. Chem. 278, 37297-37305
24. Maine, G. N., Mao, X., Komarck, C. M. and Burstein, E. (2007) COMMD1 promotes the ubiquitination of NF-κB subunits through a cullin-containing ubiquitin ligase. EMBO J. 26, 436-447