Carboxymethyl poly(L-histidine) as a new pH-sensitive polypeptide to enhance polyplex gene delivery

Molecular Pharmaceutics

Volumn 5, Issue 5, 2008, Pages 898-901

  Asayama, Shoichiro ^a   Sudo, Miyuki ^a   Nagaoka, Shoji ^a   Kawakami, Hiroyoshi ^a  

^a TOKYO METROPOLITAN UNIVERSITY   (Japan)

Author keywords

Carboxymethyl poly(L histidine); Gene delivery; pH sensitive polypeptide; Polyplex

Indexed keywords

CARBOXYMETHYLPOLYHISTIDINE; DNA; IMIDAZOLE DERIVATIVE; POLYETHYLENEIMINE; POLYPEPTIDE; UNCLASSIFIED DRUG;

AGAR GEL ELECTROPHORESIS; ARTICLE; CELL PH; COMPLEX FORMATION; CONTROLLED STUDY; ENDOSOME; GENE EXPRESSION; NONVIRAL GENE DELIVERY SYSTEM; PRIORITY JOURNAL; ZETA POTENTIAL;

CELL LINE, TUMOR; DNA; DRUG CARRIERS; ELECTROPHORESIS, AGAR GEL; ENDOCYTOSIS; ENDOSOMES; GENE TRANSFER TECHNIQUES; GENES, REPORTER; HISTIDINE; HUMANS; HYDROGEN-ION CONCENTRATION; LUCIFERASES; LYSOSOMES; PEPTIDES; PROTEINS;

EID: 56049093896     PISSN: 15438384     EISSN: 15438392     Source Type: Journal    
DOI: 10.1021/mp800094b     Document Type: Article

References (22)

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18. A typical procedure is as follows: Calf thymus DNA, purchased from Sigma Chemical Co. (St. Louis, MO), was dissolved in PBS (-) at 1.13 mg/mL. The resulting DNA stock solution was added to the polymer solutions in 50 mM sodium phosphate buffer (pH 7.5 or pH 6.0) at various polymer/DNA ratios. The final diluted concentration of DNA was adjusted to 50 μg/ mL. After 30 min of incubation at room temperature, each sample (corresponding to 0.5 μg of DNA was mixed with a loading buffer and loaded onto a 1% agarose gel containing 1 μg/mL of EtBr. Gel electrophoresis was run at room temperature in 50 mM sodium phosphate buffer (pH 7.5 or pH 6.0) at 50 V for 10 min. The DNA bands were visualized under UV irradiation.
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